W83 Traffics Via ICAM1 in Microvascular Endothelial Cells and Alters Capillary Organization

Overview

Journal J Oral Microbiol

Specialty Dentistry

Date 2020 Apr 29

PMID 32341760

Citations 14

Authors

L Reyes

H Getachew

W A Dunn

A Progulske-Fox

Affiliations

Soon will be listed here.

Abstract

Microvascular dysfunction is a feature of periodontal disease. , one of the most common oral bacteria present in gingival tissue biofilms, has also been identified in the gingival capillaries of patients with chronic periodontitis. We sought to determine the effect of W83 infection on microvascular endothelium and . Interdental papillae of rats with -induced alveolar bone loss had a more dilated and denser subepithelial capillary network than uninfected controls. W83 was detected in the epithelial layers, the subepithelial connective tissue matrix, and subgingival capillaries. invaded human dermal microvascular endothelial cells (HD-MVECS) and persisted up termination (24 h). Colocalization analysis at 2.5, 6, and 24 h post-inoculation showed that 79-88% of internalized bacteria were in ICAM-1 positive endosomes, and 10-39% were in Rab5, Rab7, or LAMP1 positive compartments, but never in autophagosomes. Antibody-based blockade of ICAM-1 significantly reduced W83 invasion in HD-MVECS. infected HD-MVECS were unable to form vascular networks in Matrigel. perturbs microvascular endothelial function and invasion of these cells via ICAM-1 may be important for microbial persistence within tissues.

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References

Clark P, Manes T, Pober J, Kluger M . Increased ICAM-1 expression causes endothelial cell leakiness, cytoskeletal reorganization and junctional alterations. J Invest Dermatol. 2006; 127(4):762-74. DOI: 10.1038/sj.jid.5700670. View

Dehio C, Meyer M, Berger J, Schwarz H, Lanz C . Interaction of Bartonella henselae with endothelial cells results in bacterial aggregation on the cell surface and the subsequent engulfment and internalisation of the bacterial aggregate by a unique structure, the invasome. J Cell Sci. 1997; 110 ( Pt 18):2141-54. DOI: 10.1242/jcs.110.18.2141. View

Rudney J, Chen R, Sedgewick G . Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis are components of a polymicrobial intracellular flora within human buccal cells. J Dent Res. 2004; 84(1):59-63. DOI: 10.1177/154405910508400110. View

Saglie F, Marfany A, Camargo P . Intragingival occurrence of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis in active destructive periodontal lesions. J Periodontol. 1988; 59(4):259-65. DOI: 10.1902/jop.1988.59.4.259. View

Muro S, Gajewski C, Koval M, Muzykantov V . ICAM-1 recycling in endothelial cells: a novel pathway for sustained intracellular delivery and prolonged effects of drugs. Blood. 2004; 105(2):650-8. DOI: 10.1182/blood-2004-05-1714. View

Saglie R, Newman M, Carranza Jr F, Pattison G . Bacterial invasion of gingiva in advanced periodontitis in humans. J Periodontol. 1982; 53(4):217-22. DOI: 10.1902/jop.1982.53.4.217. View

Hock J, Niki K . A vital microscopy study of the morphology of normal and inflamed gingiva. J Periodontal Res. 1971; 6(2):81-8. DOI: 10.1111/j.1600-0765.1971.tb00592.x. View

Rezavandi K, Palmer R, Odell E, Scott D, Wilson R . Expression of ICAM-1 and E-selectin in gingival tissues of smokers and non-smokers with periodontitis. J Oral Pathol Med. 2002; 31(1):59-64. DOI: 10.1046/j.0904-2512.2001.joptest.doc.x. View

Zhang D, Zheng H, Zhao J, Lin L, Li C, Liu J . Porphorymonas gingivalis induces intracellular adhesion molecule-1 expression in endothelial cells through the nuclear factor-kappaB pathway, but not through the p38 MAPK pathway. J Periodontal Res. 2010; 46(1):31-8. DOI: 10.1111/j.1600-0765.2010.01305.x. View

10.

Tsubokawa M, Sato S . In vitro analysis of human periodontal microvascular endothelial cells. J Periodontol. 2013; 85(8):1135-42. DOI: 10.1902/jop.2013.130209. View

11.

Johnson J, Chen R, Lenton P, Zhang G, Hinrichs J, Rudney J . Persistence of extracrevicular bacterial reservoirs after treatment of aggressive periodontitis. J Periodontol. 2008; 79(12):2305-12. PMC: 2597013. DOI: 10.1902/jop.2008.080254. View

12.

Tamatani T, Miyasaka M . Identification of monoclonal antibodies reactive with the rat homolog of ICAM-1, and evidence for a differential involvement of ICAM-1 in the adherence of resting versus activated lymphocytes to high endothelial cells. Int Immunol. 1990; 2(2):165-71. DOI: 10.1093/intimm/2.2.165. View

13.

Dorn B, Dunn Jr W, Progulske-Fox A . Porphyromonas gingivalis traffics to autophagosomes in human coronary artery endothelial cells. Infect Immun. 2001; 69(9):5698-708. PMC: 98686. DOI: 10.1128/IAI.69.9.5698-5708.2001. View

14.

Kim Y, Ko Y, Hong S, Kim K, Lee Y, Chae C . Presence of Porphyromonas gingivalis and plasma cell dominance in gingival tissues with periodontitis. Oral Dis. 2010; 16(4):375-81. DOI: 10.1111/j.1601-0825.2009.01649.x. View

15.

Muro S, Garnacho C, Champion J, Leferovich J, Gajewski C, Schuchman E . Control of endothelial targeting and intracellular delivery of therapeutic enzymes by modulating the size and shape of ICAM-1-targeted carriers. Mol Ther. 2008; 16(8):1450-8. PMC: 2810502. DOI: 10.1038/mt.2008.127. View

16.

Zoellner H, Chapple C, Hunter N . Microvasculature in gingivitis and chronic periodontitis: disruption of vascular networks with protracted inflammation. Microsc Res Tech. 2002; 56(1):15-31. DOI: 10.1002/jemt.10009. View

17.

Hsu J, Rappaport J, Muro S . Specific binding, uptake, and transport of ICAM-1-targeted nanocarriers across endothelial and subendothelial cell components of the blood-brain barrier. Pharm Res. 2014; 31(7):1855-66. PMC: 4065215. DOI: 10.1007/s11095-013-1289-8. View

18.

Tamai R, Asai Y, Ogawa T . Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis. Infect Immun. 2005; 73(10):6290-8. PMC: 1230918. DOI: 10.1128/IAI.73.10.6290-6298.2005. View

19.

Suphasiriroj W, Mikami M, Sato S . Comparative studies on microvascular endothelial cells isolated from periodontal tissue. J Periodontol. 2012; 84(7):1002-9. DOI: 10.1902/jop.2012.120453. View

20.

Shao R, Guo X . Human microvascular endothelial cells immortalized with human telomerase catalytic protein: a model for the study of in vitro angiogenesis. Biochem Biophys Res Commun. 2004; 321(4):788-94. DOI: 10.1016/j.bbrc.2004.07.033. View