The Microbial Abundance Dynamics of the Paediatric Oral Cavity Before and After Sleep

Overview

Journal J Oral Microbiol

Specialty Dentistry

Date 2020 Apr 29

PMID 32341758

Citations 8

Authors

Jessica A P Carlson-Jones

Anna Kontos

Declan Kennedy

James Martin

Kurt Lushington

Jody McKerral

James S Paterson

Renee J Smith

Lisa M Dann

Peter Speck

James G Mitchell

Affiliations

Soon will be listed here.

Abstract

: Microhabitats in the oral cavity differ in microbial taxonomy. However, abundance variations of bacterial and viral communities within these microhabitats are not fully understood. : To assess the spatial distribution and dynamics of the microbial abundances within 6 microhabitats of the oral cavity before and after sleep. We hypothesise that the abundance distributions of these microbial communities will differ among oral sites. : Using flow cytometry, bacterial and virus-like particle (VLP) abundances were enumerated for 6 oral microhabitats before and after sleep in 10 healthy paediatric sleepers. : Bacterial counts ranged from 7.2 ± 2.8 × 10 at the palate before sleep to 1.3 ± 0.2 × 10 at the back of the tongue after sleep, a difference of 187 times. VLPs ranged from 1.9 ± 1.0 × 10 at the palate before sleep to 9.2 ± 5.0 × 10 at the back of the tongue after sleep, a difference of 48 times. : The oral cavity is a dynamic numerically heterogeneous environment where microbial communities can increase by a count of 100 million during sleep. Quantification of the paediatric oral microbiome complements taxonomic diversity information to show how biomass varies and shifts in space and time.

Citing Articles

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