is Regulated by MiR-135a and Involved in DNA Damage Repair During Mouse Cellular Reprogramming

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2020 Apr 27

PMID 32335545

Citations 5

Authors

Andy Chun Hang Chen

Qian Peng

Sze Wan Fong

William Shu Biu Yeung

Yin Lau Lee

Affiliations

Soon will be listed here.

Abstract

facilitates the reprogramming of mouse somatic cells into induced pluripotent stem cells (iPSCs). It is regulated by micro-RNA and reported to be a target of miR-135a. However, their relationship and roles on cellular reprogramming remain unknown. In this study, we found negative correlations between miR-135a and during mouse embryonic stem cells differentiation and mouse embryonic fibroblasts reprogramming. We further found that the reprogramming efficiency was reduced by the overexpression of miR-135a precursor but induced by the miR-135a inhibitor. Co-immunoprecipitation followed by mass spectrometry identified 21 SIRT1 interacting proteins including KU70 and WRN, which were highly enriched for DNA damage repair. In accordance, activator resveratrol reduced DNA damage during the reprogramming process. was regulated by miR-135a and resveratrol partly rescued the impaired reprogramming efficiency induced by knockdown. This study showed , being partly regulated by miR-135a, bound proteins involved in DNA damage repair and enhanced the iPSCs production.

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