Altered Sarcomeric Structure and Function in Woody Breast Myopathy of Avian Pectoralis Major Muscle

Overview

Journal Front Physiol

Date 2020 Apr 25

PMID 32328000

Citations 10

Authors

Jiao Liu

Eero Puolanne

Matthias Schwartzkopf

Anders Arner

Affiliations

Soon will be listed here.

Abstract

The "Woody" or "Wooden" breast disease is a severe myopathy of pectoralis major muscle recently identified within rapidly growing broiler lines all around the world with a prevalence rate around 20%, or even higher. Although of significant ethical and economic impact, little is known regarding the structural and functional aspects of the contractile apparatus in the woody breast muscle. The aim of the present study was to determine physiological properties of the contractile system in the morphologically intact muscle fibers of focally damaged woody breast in comparison with normal muscle fibers to gain insight into the muscle function of the animal and possibly mechanisms involved in the disease development. Muscle samples were taken from woody breast (non-lesioned areas) and normal breast muscles from broilers. Length-tension curves, maximal active stress, maximal shortening velocity, calcium sensitivity, rate of tension development, lattice spacing and muscle biochemical composition were investigated on single skinned fibers. Sarcomeres of woody breast fibers were more compliant, which is very likely related to the wider spacing (18% wider compared to controls) between thick and thin filament. No differences were found in optimal sarcomere length (2.68 ± 0.04 2.65 ± 0.05 μm) nor in maximal active stress (116 ± 17 125 ± 19 mN mm). However, woody breast fibers had less steep descending arm as shown in length-tension curve. Woody breast muscle fibers had 40% bigger sarcomeric volume compared to controls. Content of contractile proteins (myosin and actin), and maximal shortening velocity were unchanged indicating that the growth in woody breast muscle fiber was associated with synthesis of new contractile units with unaltered kinetics. Calcium sensitivity was decreased in woody breast muscle fibers significantly. In conclusion, the results show that the rapid growth of muscle in woody breast disease is associated with significant structural and functional changes in the pectoralis major musculature, associated with alterations in the mechanical anchoring of contractile filaments.

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References

Baldi G, Soglia F, Mazzoni M, Sirri F, Canonico L, Babini E . Implications of white striping and spaghetti meat abnormalities on meat quality and histological features in broilers. Animal. 2017; 12(1):164-173. DOI: 10.1017/S1751731117001069. View

Burkholder T, Lieber R . Sarcomere length operating range of vertebrate muscles during movement. J Exp Biol. 2001; 204(Pt 9):1529-36. DOI: 10.1242/jeb.204.9.1529. View

Lange S, Xiang F, Yakovenko A, Vihola A, Hackman P, Rostkova E . The kinase domain of titin controls muscle gene expression and protein turnover. Science. 2005; 308(5728):1599-603. DOI: 10.1126/science.1110463. View

Trocino A, Piccirillo A, Birolo M, Radaelli G, Bertotto D, Filiou E . Effect of genotype, gender and feed restriction on growth, meat quality and the occurrence of white striping and wooden breast in broiler chickens. Poult Sci. 2015; 94(12):2996-3004. DOI: 10.3382/ps/pev296. View

Berri C, Bihan-Duval E, Debut M, Sante-Lhoutellier V, Baeza E, Gigaud V . Consequence of muscle hypertrophy on characteristics of Pectoralis major muscle and breast meat quality of broiler chickens. J Anim Sci. 2007; 85(8):2005-11. DOI: 10.2527/jas.2006-398. View

HUXLEY A . Muscle structure and theories of contraction. Prog Biophys Biophys Chem. 1957; 7:255-318. View

Remignon H, Gardahaut M, Marche G, Ricard F . Selection for rapid growth increases the number and the size of muscle fibres without changing their typing in chickens. J Muscle Res Cell Motil. 1995; 16(2):95-102. DOI: 10.1007/BF00122527. View

Clark D, Velleman S . Spatial influence on breast muscle morphological structure, myofiber size, and gene expression associated with the wooden breast myopathy in broilers. Poult Sci. 2016; 95(12):2930-2945. DOI: 10.3382/ps/pew243. View

Soglia F, Mazzoni M, Zappaterra M, Di Nunzio M, Babini E, Bordini M . Distribution and Expression of Vimentin and Desmin in Broiler Affected by the Growth-Related Muscular Abnormalities. Front Physiol. 2020; 10:1581. PMC: 6978684. DOI: 10.3389/fphys.2019.01581. View

10.

Sihvo H, Linden J, Airas N, Immonen K, Valaja J, Puolanne E . Wooden Breast Myodegeneration of Pectoralis Major Muscle Over the Growth Period in Broilers. Vet Pathol. 2016; 54(1):119-128. DOI: 10.1177/0300985816658099. View

11.

Rome L, Cook C, Syme D, Connaughton M, Klimov A, Tikunov B . Trading force for speed: why superfast crossbridge kinetics leads to superlow forces. Proc Natl Acad Sci U S A. 1999; 96(10):5826-31. PMC: 21945. DOI: 10.1073/pnas.96.10.5826. View

12.

Reiser P, Greaser M, Moss R . Developmental changes in troponin T isoform expression and tension production in chicken single skeletal muscle fibres. J Physiol. 1992; 449:573-88. PMC: 1176095. DOI: 10.1113/jphysiol.1992.sp019102. View

13.

Reiser P, Greaser M, Moss R . Myosin heavy chain composition of single cells from avian slow skeletal muscle is strongly correlated with velocity of shortening during development. Dev Biol. 1988; 129(2):400-7. DOI: 10.1016/0012-1606(88)90387-9. View

14.

MILLMAN B . The filament lattice of striated muscle. Physiol Rev. 1998; 78(2):359-91. DOI: 10.1152/physrev.1998.78.2.359. View

15.

EDMAN K . The force bearing capacity of frog muscle fibres during stretch: its relation to sarcomere length and fibre width. J Physiol. 1999; 519 Pt 2:515-26. PMC: 2269524. DOI: 10.1111/j.1469-7793.1999.0515m.x. View

16.

Mudalal S, Lorenzi M, Soglia F, Cavani C, Petracci M . Implications of white striping and wooden breast abnormalities on quality traits of raw and marinated chicken meat. Animal. 2014; 9(4):728-34. DOI: 10.1017/S175173111400295X. View

17.

Norring M, Valros A, Valaja J, Sihvo H, Immonen K, Puolanne E . Wooden breast myopathy links with poorer gait in broiler chickens. Animal. 2018; 13(8):1690-1695. DOI: 10.1017/S1751731118003270. View

18.

Liu J, Schwartzkopf M, Arner A . Rigor bonds cause reduced sarcomeric volume in skinned porcine skeletal muscle under PSE-like conditions. Meat Sci. 2018; 139:91-96. DOI: 10.1016/j.meatsci.2018.01.014. View

19.

Iorga B, Wang L, Stehle R, Pfitzer G, Kawai M . ATP binding and cross-bridge detachment steps during full Ca²⁺ activation: comparison of myofibril and muscle fibre mechanics by sinusoidal analysis. J Physiol. 2012; 590(14):3361-73. PMC: 3459048. DOI: 10.1113/jphysiol.2012.228379. View

20.

Sihvo H, Immonen K, Puolanne E . Myodegeneration with fibrosis and regeneration in the pectoralis major muscle of broilers. Vet Pathol. 2013; 51(3):619-23. DOI: 10.1177/0300985813497488. View