and Protozoan Hosts: Implications for the Control of Hospital and Potable Water Systems

Overview

Journal Pathogens

Date 2020 Apr 25

PMID 32326561

Citations 17

Authors

Muhammad Atif Nisar

Kirstin E Ross

Melissa H Brown

Richard Bentham

Harriet Whiley

Affiliations

Soon will be listed here.

Abstract

is an opportunistic waterborne pathogen of public health concern. It is the causative agent of Legionnaires' disease (LD) and Pontiac fever and is ubiquitous in manufactured water systems, where protozoan hosts and complex microbial communities provide protection from disinfection procedures. This review collates the literature describing interactions between and protozoan hosts in hospital and municipal potable water distribution systems. The effectiveness of currently available water disinfection protocols to control and its protozoan hosts is explored. The studies identified in this systematic literature review demonstrated the failure of common disinfection procedures to achieve long term elimination of and protozoan hosts from potable water. It has been demonstrated that protozoan hosts facilitate the intracellular replication and packaging of viable in infectious vesicles; whereas, cyst-forming protozoans provide protection from prolonged environmental stress. Disinfection procedures and protozoan hosts also facilitate biogenesis of viable but non-culturable (VBNC) which have been shown to be highly resistant to many water disinfection protocols. In conclusion, a better understanding of -protozoan interactions and the structure of complex microbial biofilms is required for the improved management of and the prevention of LD.

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References

Critchley M, Bentham R . The efficacy of biocides and other chemical additives in cooling water systems in the control of amoebae. J Appl Microbiol. 2009; 106(3):784-9. DOI: 10.1111/j.1365-2672.2008.04044.x. View

Yanez M, Nocker A, Soria-Soria E, Murtula R, Martinez L, Catalan V . Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR. J Microbiol Methods. 2011; 85(2):124-30. DOI: 10.1016/j.mimet.2011.02.004. View

Lagana P, Caruso G, Piccione D, Gioffre M, Pino R, Delia S . Legionella spp., amoebae and not-fermenting Gram negative bacteria in an Italian university hospital water system. Ann Agric Environ Med. 2014; 21(3):489-93. DOI: 10.5604/12321966.1120623. View

Dietersdorfer E, Cervero-Arago S, Sommer R, Kirschner A, Walochnik J . Optimized methods for Legionella pneumophila release from its Acanthamoeba hosts. BMC Microbiol. 2016; 16:74. PMC: 4845434. DOI: 10.1186/s12866-016-0691-x. View

Newsome A, Baker R, Miller R, Arnold R . Interactions between Naegleria fowleri and Legionella pneumophila. Infect Immun. 1985; 50(2):449-52. PMC: 261973. DOI: 10.1128/iai.50.2.449-452.1985. View

Thomas J, Ashbolt N . Do free-living amoebae in treated drinking water systems present an emerging health risk?. Environ Sci Technol. 2011; 45(3):860-9. DOI: 10.1021/es102876y. View

Garcia M, Jones S, Pelaz C, Millar R, Abu Kwaik Y . Acanthamoeba polyphaga resuscitates viable non-culturable Legionella pneumophila after disinfection. Environ Microbiol. 2007; 9(5):1267-77. DOI: 10.1111/j.1462-2920.2007.01245.x. View

Watanabe K, Nakao R, Fujishima M, Tachibana M, Shimizu T, Watarai M . Ciliate Paramecium is a natural reservoir of Legionella pneumophila. Sci Rep. 2016; 6:24322. PMC: 4832178. DOI: 10.1038/srep24322. View

Lau H, Ashbolt N . The role of biofilms and protozoa in Legionella pathogenesis: implications for drinking water. J Appl Microbiol. 2009; 107(2):368-78. DOI: 10.1111/j.1365-2672.2009.04208.x. View

10.

Prussin 2nd A, Schwake D, Marr L . Ten Questions Concerning the Aerosolization and Transmission of Legionella in the Built Environment. Build Environ. 2017; 123:684-695. PMC: 5665586. DOI: 10.1016/j.buildenv.2017.06.024. View

11.

Phin N, Parry-Ford F, Harrison T, Stagg H, Zhang N, Kumar K . Epidemiology and clinical management of Legionnaires' disease. Lancet Infect Dis. 2014; 14(10):1011-21. DOI: 10.1016/S1473-3099(14)70713-3. View

12.

Berk S, Faulkner G, Garduno E, Joy M, Ortiz-Jimenez M, Garduno R . Packaging of live Legionella pneumophila into pellets expelled by Tetrahymena spp. does not require bacterial replication and depends on a Dot/Icm-mediated survival mechanism. Appl Environ Microbiol. 2008; 74(7):2187-99. PMC: 2292602. DOI: 10.1128/AEM.01214-07. View

13.

Wang H, Xu J, Tang W, Li H, Xia S, Zhao J . Removal Efficacy of Opportunistic Pathogens and Bacterial Community Dynamics in Two Drinking Water Treatment Trains. Small. 2018; 15(2):e1804436. DOI: 10.1002/smll.201804436. View

14.

Thomas V, Bouchez T, Nicolas V, Robert S, Loret J, Levi Y . Amoebae in domestic water systems: resistance to disinfection treatments and implication in Legionella persistence. J Appl Microbiol. 2004; 97(5):950-63. DOI: 10.1111/j.1365-2672.2004.02391.x. View

15.

Nahapetian K, Challemel O, Beurtin D, Dubrou S, Gounon P, Squinazi F . The intracellular multiplication of Legionella pneumophila in protozoa from hospital plumbing systems. Res Microbiol. 1991; 142(6):677-85. DOI: 10.1016/0923-2508(91)90081-k. View

16.

Nocker A, Cheung C, Camper A . Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J Microbiol Methods. 2006; 67(2):310-20. DOI: 10.1016/j.mimet.2006.04.015. View

17.

Benedict K, Reses H, Vigar M, Roth D, Roberts V, Mattioli M . Surveillance for Waterborne Disease Outbreaks Associated with Drinking Water - United States, 2013-2014. MMWR Morb Mortal Wkly Rep. 2017; 66(44):1216-1221. PMC: 5679581. DOI: 10.15585/mmwr.mm6644a3. View

18.

Jjemba P, Johnson W, Bukhari Z, LeChevallier M . Occurrence and Control of Legionella in Recycled Water Systems. Pathogens. 2015; 4(3):470-502. PMC: 4584268. DOI: 10.3390/pathogens4030470. View

19.

Liu Z, Lin Y, Stout J, Hwang C, Vidic R, Yu V . Effect of flow regimes on the presence of Legionella within the biofilm of a model plumbing system. J Appl Microbiol. 2006; 101(2):437-42. DOI: 10.1111/j.1365-2672.2006.02970.x. View

20.

Lu J, Struewing I, Vereen E, Kirby A, Levy K, Moe C . Molecular Detection of Legionella spp. and their associations with Mycobacterium spp., Pseudomonas aeruginosa and amoeba hosts in a drinking water distribution system. J Appl Microbiol. 2015; 120(2):509-21. DOI: 10.1111/jam.12996. View