Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics

Overview

Journal Animals (Basel)

Date 2020 Apr 25

PMID 32326487

Citations 6

Authors

Aleksandra Dunislawska

Maria Siwek

Anna Slawinska

Adam Lepczynski

Agnieszka Herosimczyk

Pawel A Kolodziejski

Marek Bednarczyk

Affiliations

Soon will be listed here.

Abstract

To better understand the effects of synbiotics administered at early stages of embryonic development in poultry, it is necessary to analyze direct effects (meat quality) and the molecular background. The molecular interpretation of poultry meat properties after administration of synbiotics remains to be reported. The purpose of the present study was to analyze the molecular background of meat quality based on gene expression and basic physiological parameters. Eggs were injected with (S1) with galacto-oligosaccharides or (S2) with raffinose family oligosaccharides. The pectoral muscle was collected at two time points (day 7 and day 42) and subjected to RNA isolation. Gene expression analysis was performed by RT-qPCR for a panel of eight genes associated with metabolism. The concentration of glucose and hormones (insulin, glucagon, and leptin (S1 = 0.04)) was also increased. The obtained results showed that metabolic gene expression in the muscle was more differential due to synbiotic stimulation on day 7 ( in S1 = 0.03; in S1 = 0.02 and S2 = 0.01; in S1 = 0.01 and S2 0.008; PHKB in S1 0.01; in S1 0.02) than on day 42 ( in S1 0.04). On the basis of the results obtained, it can be concluded that stimulation with S1 triggered the most potent and favorable changes in the pectoral muscle gene expression in broiler chickens.

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