Increased Expression of CD8 Marker on T-cells in COVID-19 Patients

Overview

Journal Blood Cells Mol Dis

Specialty Hematology

Date 2020 Apr 24

PMID 32325421

Citations 81

Authors

Ali Ganji

Iman Farahani

Behzad Khansarinejad

Ali Ghazavi

Ghasem Mosayebi

Affiliations

Soon will be listed here.

Abstract

Background: Cell-mediated immunity including T-cells (T helper and cytotoxic) plays an essential role in efficient antiviral responses against coronavirus disease-2019 (COVID-19). Therefore, in this study, we evaluated the ratio and expression of CD4 and CD8 markers in COVID-19 patients to clarify the immune characterizations of CD4 and CD8 T-cells in COVID-19 patients.

Methods: Peripheral blood samples of 25 COVID-19 patients and 25 normal individuals with similar age and sex as the control group were collected. White blood cells, platelets, and lymphocytes were counted and CD4 and CD8 T lymphocytes were evaluated by flow cytometry.

Results: The number of white blood cells, lymphocytes, and platelets were reduced significantly in COVID-19 patients (P < 0.05). The difference in CD4:CD8 ratio, CD4 T-cell frequency, CD8 T-cell frequency, and CD4 mean fluorescence intensity (MFI) was not significant between COVID-19 patients and healthy individuals (P > 0.05); however, the CD8 MFI increased significantly in COVID-19 infected patients (P < 0.05).

Conclusion: Although, there is no significant difference in the ratio of CD4 to CD8 between two groups, the expression level of CD8 in COVID-19 patients was significantly higher than the normal individuals. This result suggested that the cellular immune responses triggered by COVID-19 infection were developed through overexpression of CD8 and hyperactivation of cytotoxic T lymphocytes.

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References

McBride J, Striker R . Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health?. PLoS Pathog. 2017; 13(11):e1006624. PMC: 5667733. DOI: 10.1371/journal.ppat.1006624. View

Xu Z, Shi L, Wang Y, Zhang J, Huang L, Zhang C . Pathological findings of COVID-19 associated with acute respiratory distress syndrome. Lancet Respir Med. 2020; 8(4):420-422. PMC: 7164771. DOI: 10.1016/S2213-2600(20)30076-X. View

Lippi G, Plebani M, Henry B . Thrombocytopenia is associated with severe coronavirus disease 2019 (COVID-19) infections: A meta-analysis. Clin Chim Acta. 2020; 506:145-148. PMC: 7102663. DOI: 10.1016/j.cca.2020.03.022. View

Jung M, Pape G . Immunology of hepatitis B infection. Lancet Infect Dis. 2002; 2(1):43-50. DOI: 10.1016/s1473-3099(01)00172-4. View

Hu B, Zeng L, Yang X, Ge X, Zhang W, Li B . Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus. PLoS Pathog. 2017; 13(11):e1006698. PMC: 5708621. DOI: 10.1371/journal.ppat.1006698. View

Mescher M, Curtsinger J, Agarwal P, Casey K, Gerner M, Hammerbeck C . Signals required for programming effector and memory development by CD8+ T cells. Immunol Rev. 2006; 211:81-92. DOI: 10.1111/j.0105-2896.2006.00382.x. View

Wang D, Hu B, Hu C, Zhu F, Liu X, Zhang J . Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus-Infected Pneumonia in Wuhan, China. JAMA. 2020; 323(11):1061-1069. PMC: 7042881. DOI: 10.1001/jama.2020.1585. View

Chen N, Zhou M, Dong X, Qu J, Gong F, Han Y . Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. Lancet. 2020; 395(10223):507-513. PMC: 7135076. DOI: 10.1016/S0140-6736(20)30211-7. View

Song H, Tu C, Zhang G, Wang S, Zheng K, Lei L . Cross-host evolution of severe acute respiratory syndrome coronavirus in palm civet and human. Proc Natl Acad Sci U S A. 2005; 102(7):2430-5. PMC: 548959. DOI: 10.1073/pnas.0409608102. View

10.

Zaunders J, Carr A, McNally L, Penny R, Cooper D . Effects of primary HIV-1 infection on subsets of CD4+ and CD8+ T lymphocytes. AIDS. 1995; 9(6):561-6. DOI: 10.1097/00002030-199506000-00005. View

11.

Zhu J, Yamane H, Paul W . Differentiation of effector CD4 T cell populations (*). Annu Rev Immunol. 2010; 28:445-89. PMC: 3502616. DOI: 10.1146/annurev-immunol-030409-101212. View

12.

Paltrinieri S, Rossi G, Giordano A . Relationship between rate of infection and markers of inflammation/immunity in Holy Birman cats with feline coronavirus. Res Vet Sci. 2014; 97(2):263-70. PMC: 7111858. DOI: 10.1016/j.rvsc.2014.08.009. View

13.

Jason J, Inge K . Modulation of CD8 and CD3 by HIV or HIV antigens. Scand J Immunol. 2001; 53(3):259-67. DOI: 10.1046/j.1365-3083.2001.00871.x. View

14.

Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y . Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020; 395(10223):497-506. PMC: 7159299. DOI: 10.1016/S0140-6736(20)30183-5. View

15.

Diao B, Wang C, Tan Y, Chen X, Liu Y, Ning L . Reduction and Functional Exhaustion of T Cells in Patients With Coronavirus Disease 2019 (COVID-19). Front Immunol. 2020; 11:827. PMC: 7205903. DOI: 10.3389/fimmu.2020.00827. View