Antithrombotic Effect and Action Mechanism of and Herbal Pair on the Zebrafish

Overview

Journal Chin Med

Publisher Biomed Central

Specialty Biomedical Engineering

Date 2020 Apr 24

PMID 32322295

Citations 7

Authors

Shi-Jun Yin

Ying-Qing Luo

Cong-Peng Zhao

Hua Chen

Zhang-Feng Zhong

Shengpeng Wang

Yi-Tao Wang

Feng-Qing Yang

Affiliations

Soon will be listed here.

Abstract

Background: (Danshen, DS) and (Sanqi, SQ) are famous traditional Chinese herbs, and their herbal pair (DS-SQ) has been popular used as anti-thrombotic medicines. However, there is still a lack of sufficient scientific evidence to illustrate the optimum combination ratio of these two herbs as well as its action mechanisms. The purpose of this study is to investigate the anti-thrombotic effects of DS-SQ on zebrafish and explore its possible action mechanism.

Methods: Firstly, the chemical components in DS-SQ extract were analyzed by LC-ESI-MS/MS. Then, a phenylhydrazine (PHZ)-induced zebrafish thrombosis model was developed for evaluating the anti-thrombotic effects of DS-SQ extracts with different combination ratios and their nine pure compounds. Followed, Real-time quantitative PCR (RT-qPCR) assays were performed to investigate the potential antithrombotic mechanisms of DS-SQ.

Results: Thirty-three components were tentatively identified by LC-MS analysis. DS-SQ at the ratio of 10:1 presented the best anti-thrombotic effect, and rosmarinic acid, lithospermic acid and salvianolic acid B of DS showed good anti-thrombotic activity on zebrafish thrombosis model. The RT-qPCR assays indicated that DS-SQ (10:1) could cure the PHZ-induced thrombosis by downregulating the expression of , , , , and in zebrafish.

Conclusions: DS-SQ with the combination ratio of 10:1 showed optimum anti-thrombotic effect on PHZ-induced zebrafish thrombosis model, which provided a reference for reasonable clinical applications of DS-SQ herbal pair.

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