A Bioorthogonal Chemical Reporter of Viral Infection

Overview

Journal Angew Chem Weinheim Bergstr Ger

Date 2020 Apr 22

PMID 32313318

Citations 3

Authors

Anne B Neef

Lucile Pernot

Verena N Schreier

Leonardo Scapozza

Nathan W Luedtke

Affiliations

Soon will be listed here.

Abstract

Pathogen-selective labeling was achieved by using the novel gemcitabine metabolite analogue 2'-deoxy-2',2'-difluoro-5-ethynyluridine (dF-EdU) and click chemistry. Cells infected with Herpes Simplex Virus-1 (HSV-1), but not uninfected cells, exhibit nuclear staining upon the addition of dF-EdU and a fluorescent azide. The incorporation of the dF-EdU into DNA depends on its phosphorylation by a herpes virus thymidine kinase (TK). Crystallographic analyses revealed how dF-EdU is well accommodated in the active site of HSV-1 TK, but steric clashes prevent dF-EdU from binding human TK. These results provide the first example of pathogen-enzyme-dependent incorporation and labeling of bioorthogonal functional groups in human cells.

Citing Articles

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Metabolic Incorporation of Azide Functionality into Cellular RNA.

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