Salivary Interleukin-6 Levels Among Polycystic Ovary Syndrome Patients with and Without Chronic Periodontitis - A Comparative Study

Overview

Journal Contemp Clin Dent

Specialty Dentistry

Date 2020 Apr 21

PMID 32308327

Citations 4

Authors

Arun Varghese

Pratebha Balu

Saravanakumar R

Jananni Muthu

K Vineela

Affiliations

Soon will be listed here.

Abstract

Background: Periodontitis is associated with various systemic diseases one of which is poly cystic ovarian syndrome (PCOS). PCOS is a genetically complex endocrinopathy of uncertain etiology affecting women of the reproductive age group which results in the most common cause of anovulatory infertility, menstrual dysfunction, and hirsutism. PCOS has a close association with cardiometabolic risk profile, insulin resistance (IR), hyperinsulinemia, central obesity, dyslipidemia, and increasing the prevalence of cardiovascular risk factors. The common pathway is the chronic low-grade inflammation which is constituted by pro-inflammatory cytokine interleukin (IL)-6.

Aim: The aim of the study was to compare salivary IL-6 levels among polycystic ovary syndrome (PCOS) patients with and without chronic periodontitis.

Materials And Methods: Newly diagnosed PCOS patients were selected for the study, and the periodontal parameters were recorded. Group A consists of 42 patients of PCOS with periodontitis and Group B consists of 42 patients of PCOS without periodontitis. Salivary levels of IL-6 were compared between the two groups and were assessed by enzyme-linked immunosorbent assay kit (bioassay).

Results: The mean pocket depth in Group A was 4.23 ± 0.134 and that of Group B was 1.30 ± 0.06. The mean bleeding on probing in Group A was 1.40 ± 0.40 and in Group B it was 0.91 ± 0.18. The mean clinical attachment level in Group A was 4.87 ± 0.124 and that of Group B was 1.30 ± 0.06. The mean difference in clinical parameters was statistically significant between the groups ( ≤ 0.001). IL-6 level in group A is 102.59 ± 18.2 and in Group B it was 51.3 ± 25.3.

Conclusion: Salivary IL-6 levels show a double-fold increase in PCOS with periodontitis than in PCOS without periodontitis. This study reflects the importance of periodontal health and the prevention of periodontal disease so as to minimize IR in PCOS patients with periodontitis.

Citing Articles

Research progress on the potential correlation between polycystic ovary syndrome and periodontal disease.

Zhang H, Lin X J Int Med Res. 2024; 52(11):3000605241300096.

PMID: 39600040 PMC: 11603522. DOI: 10.1177/03000605241300096.

Anti-inflammatory effect of Faloak ( R. Br) stem bark on TNF-α, IL-1β, and IL-6 in DENV-3-infected Wistar rats.

Riwu A, Nugraha J, Triyono E, Purwanto D Open Vet J. 2024; 14(4):1043-1050.

PMID: 38808285 PMC: 11128639. DOI: 10.5455/OVJ.2024.v14.i4.11.

Investigation of the mechanisms and experimental verification of Cuscuta-Salvia in the treatment of polycystic ovary syndrome (PCOS) via network pharmacology.

Zhang Y, Ma J, Zhu Y, Wang Y, Chen W, Sun X J Ovarian Res. 2022; 15(1):40.

PMID: 35379295 PMC: 8978390. DOI: 10.1186/s13048-022-00964-8.

Is There a Bidirectional Association between Polycystic Ovarian Syndrome and Periodontitis? A Systematic Review and Meta-analysis.

Machado V, Escalda C, Proenca L, Mendes J, Botelho J J Clin Med. 2020; 9(6).

PMID: 32585861 PMC: 7355910. DOI: 10.3390/jcm9061961.

References

Baillargeon J, Iuorno M, Nestler J . Insulin sensitizers for polycystic ovary syndrome. Clin Obstet Gynecol. 2003; 46(2):325-40. DOI: 10.1097/00003081-200306000-00011. View

Taba Jr M, Jin Q, Sugai J, Giannobile W . Current concepts in periodontal bioengineering. Orthod Craniofac Res. 2005; 8(4):292-302. PMC: 2581520. DOI: 10.1111/j.1601-6343.2005.00352.x. View

. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS). Hum Reprod. 2003; 19(1):41-7. DOI: 10.1093/humrep/deh098. View

Newbrun E . Indices to measure gingival bleeding. J Periodontol. 1996; 67(6):555-61. DOI: 10.1902/jop.1996.67.6.555. View

Deepti , Tewari S, Narula S, Singhal S, Sharma R . Effect of Non-Surgical Periodontal Therapy Along With Myo-Inositol on High-Sensitivity C-Reactive Protein and Insulin Resistance in Women With Polycystic Ovary Syndrome and Chronic Periodontitis: A Randomized Controlled Trial. J Periodontol. 2017; 88(10):999-1011. DOI: 10.1902/jop.2017.170121. View

Gabriela Teixeira F, Mendonca S, Menezes Oliveira K, Dos Santos D, Marques L, Amorim M . Interleukin-6 c.-174G>C Polymorphism and Periodontitis in a Brazilian Population. Mol Biol Int. 2014; 2014:490308. PMC: 4274816. DOI: 10.1155/2014/490308. View

Kelly C, Lyall H, Petrie J, Gould G, Connell J, Sattar N . Low grade chronic inflammation in women with polycystic ovarian syndrome. J Clin Endocrinol Metab. 2001; 86(6):2453-5. DOI: 10.1210/jcem.86.6.7580. View

Shen Y, Guo S, Wu Y . [Oxidative stress and antioxitant therapy of chronic periodontitis]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2016; 51(7):442-6. DOI: 10.3760/cma.j.issn.1002-0098.2016.07.013. View

Peng Z, Sun Y, Lv X, Zhang H, Liu C, Dai S . Interleukin-6 Levels in Women with Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis. PLoS One. 2016; 11(2):e0148531. PMC: 4746122. DOI: 10.1371/journal.pone.0148531. View

10.

Legro R . Polycystic ovary syndrome and cardiovascular disease: a premature association?. Endocr Rev. 2003; 24(3):302-12. DOI: 10.1210/er.2003-0004. View

11.

Vidal F, Figueredo C, Cordovil I, Fischer R . Periodontal therapy reduces plasma levels of interleukin-6, C-reactive protein, and fibrinogen in patients with severe periodontitis and refractory arterial hypertension. J Periodontol. 2009; 80(5):786-91. DOI: 10.1902/jop.2009.080471. View

12.

Cekici A, Kantarci A, Hasturk H, Van Dyke T . Inflammatory and immune pathways in the pathogenesis of periodontal disease. Periodontol 2000. 2013; 64(1):57-80. PMC: 4500791. DOI: 10.1111/prd.12002. View

13.

Blank S, Helm K, McCartney C, Marshall J . Polycystic ovary syndrome in adolescence. Ann N Y Acad Sci. 2008; 1135:76-84. DOI: 10.1196/annals.1429.005. View

14.

Uitto V, Overall C, McCulloch C . Proteolytic host cell enzymes in gingival crevice fluid. Periodontol 2000. 2003; 31:77-104. DOI: 10.1034/j.1600-0757.2003.03106.x. View

15.

Frodge B, Ebersole J, Kryscio R, Thomas M, Miller C . Bone remodeling biomarkers of periodontal disease in saliva. J Periodontol. 2008; 79(10):1913-9. DOI: 10.1902/jop.2008.080070. View

16.

Porwal S, Tewari S, Sharma R, Singhal S, Narula S . Periodontal status and high-sensitivity C-reactive protein levels in polycystic ovary syndrome with and without medical treatment. J Periodontol. 2014; 85(10):1380-9. DOI: 10.1902/jop.2014.130756. View

17.

Pellegrini G, Gonzales Chaves M, Fajardo M, Ponce G, Toyos G, Lifshitz F . Salivary bone turnover markers in healthy pre- and postmenopausal women: daily and seasonal rhythm. Clin Oral Investig. 2011; 16(2):651-7. DOI: 10.1007/s00784-011-0538-7. View

18.

Tarkun I, Cetinarslan B, Turemen E, Canturk Z, Biyikli M . Association between Circulating Tumor Necrosis Factor-Alpha, Interleukin-6, and Insulin Resistance in Normal-Weight Women with Polycystic Ovary Syndrome. Metab Syndr Relat Disord. 2008; 4(2):122-8. DOI: 10.1089/met.2006.4.122. View

19.

Franks S . Polycystic ovary syndrome in adolescents. Int J Obes (Lond). 2008; 32(7):1035-41. DOI: 10.1038/ijo.2008.61. View

20.

Senn J, Klover P, Nowak I, Mooney R . Interleukin-6 induces cellular insulin resistance in hepatocytes. Diabetes. 2002; 51(12):3391-9. DOI: 10.2337/diabetes.51.12.3391. View