Prior Exercise in Humans Redistributes Intramuscular GLUT4 and Enhances Insulin-stimulated Sarcolemmal and Endosomal GLUT4 Translocation

Overview

Journal Mol Metab

Specialty Cell Biology

Date 2020 Apr 20

PMID 32305516

Citations 13

Authors

Jonas R Knudsen

Dorte E Steenberg

Janne R Hingst

Lorna R Hodgson

Carlos Henriquez-Olguin

Zhencheng Li

Bente Kiens

Erik A Richter

Jorgen F P Wojtaszewski

Paul Verkade

Thomas E Jensen

Affiliations

Soon will be listed here.

Abstract

Objective: Exercise is a cornerstone in the management of skeletal muscle insulin-resistance. A well-established benefit of a single bout of exercise is increased insulin sensitivity for hours post-exercise in the previously exercised musculature. Although rodent studies suggest that the insulin-sensitization phenomenon involves enhanced insulin-stimulated GLUT4 cell surface translocation and might involve intramuscular redistribution of GLUT4, the conservation to humans is unknown.

Methods: Healthy young males underwent an insulin-sensitizing one-legged kicking exercise bout for 1 h followed by fatigue bouts to exhaustion. Muscle biopsies were obtained 4 h post-exercise before and after a 2-hour hyperinsulinemic-euglycemic clamp.

Results: A detailed microscopy-based analysis of GLUT4 distribution within seven different myocellular compartments revealed that prior exercise increased GLUT4 localization in insulin-responsive storage vesicles and T-tubuli. Furthermore, insulin-stimulated GLUT4 localization was augmented at the sarcolemma and in the endosomal compartments.

Conclusions: An intracellular redistribution of GLUT4 post-exercise is proposed as a molecular mechanism contributing to the insulin-sensitizing effect of prior exercise in human skeletal muscle.

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