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Overview

Journal Regen Biomater

Specialty Biomedical Engineering

Date 2020 Apr 17

PMID 32296532

Citations 7

Authors

Xinda Li

Dezhi Zhou

Zhizhong Jin

Hongqing Chen

Xuanzhi Wang

Xinzhi Zhang

Tao Xu

Affiliations

Soon will be listed here.

Abstract

Cellular therapies play a critical role in the treatment of spinal cord injury (SCI). Compared with cell-seeded conduits, fully cellular grafts have more similarities with autografts, and thus might result in better regeneration effects. In this study, we fabricated Schwann cell (SC)-neural stem cell (NSC) core-shell alginate hydrogel fibers in a coaxial extrusion manner. The rat SC line RSC96 and mouse NSC line NE-4C were used in this experiment. Fully cellular components were achieved in the core portion and the relative spatial positions of these two cells partially mimic the construction of nerve fibers . SCs were demonstrated to express more genes of neurotrophic factors in alginate shell. Enhanced proliferation and differentiation tendency of NSCs was observed when they were co-cultured with SCs. This model has strong potential for application in SCI repair.

Citing Articles

Biomaterials for neuroengineering: applications and challenges.

Wu H, Feng E, Yin H, Zhang Y, Chen G, Zhu B Regen Biomater. 2025; 12:rbae137.

PMID: 40007617 PMC: 11855295. DOI: 10.1093/rb/rbae137.

The influence of tag sequence on recombinant humanized collagen (rhCol) and the evaluation of rhCol on Schwann cell behaviors.

Bai M, Kang N, Xu Y, Wang J, Shuai X, Liu C Regen Biomater. 2023; 10:rbad089.

PMID: 38020236 PMC: 10676520. DOI: 10.1093/rb/rbad089.

Recent advances in regenerative biomaterials.

Cao D, Ding J Regen Biomater. 2022; 9:rbac098.

PMID: 36518879 PMC: 9745784. DOI: 10.1093/rb/rbac098.

Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering.

Rojek K, Cwiklinska M, Kuczak J, Guzowski J Chem Rev. 2022; 122(22):16839-16909.

PMID: 36108106 PMC: 9706502. DOI: 10.1021/acs.chemrev.1c00798.

3D Bioprinting of Prevascularized Full-Thickness Gelatin-Alginate Structures with Embedded Co-Cultures.

Bottcher B, Pflieger A, Schumacher J, Jungnickel B, Feller K Bioengineering (Basel). 2022; 9(6).

PMID: 35735485 PMC: 9219913. DOI: 10.3390/bioengineering9060242.

References

Ishii T, Kawakami E, Endo K, Misawa H, Watabe K . Myelinating cocultures of rodent stem cell line-derived neurons and immortalized Schwann cells. Neuropathology. 2017; 37(5):475-481. DOI: 10.1111/neup.12397. View

Reakasame S, Trapani D, Detsch R, Boccaccini A . Cell laden alginate-keratin based composite microcapsules containing bioactive glass for tissue engineering applications. J Mater Sci Mater Med. 2018; 29(12):185. DOI: 10.1007/s10856-018-6195-5. View

Fu S, Gordon T . The cellular and molecular basis of peripheral nerve regeneration. Mol Neurobiol. 1997; 14(1-2):67-116. DOI: 10.1007/BF02740621. View

Sugai K, Nishimura S, Kato-Negishi M, Onoe H, Iwanaga S, Toyama Y . Neural stem/progenitor cell-laden microfibers promote transplant survival in a mouse transected spinal cord injury model. J Neurosci Res. 2015; 93(12):1826-38. DOI: 10.1002/jnr.23636. View

Ning L, Guillemot A, Zhao J, Kipouros G, Chen X . Influence of Flow Behavior of Alginate-Cell Suspensions on Cell Viability and Proliferation. Tissue Eng Part C Methods. 2016; 22(7):652-62. DOI: 10.1089/ten.TEC.2016.0011. View

Yu Z, Men Y, Dong P . Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors. Exp Ther Med. 2017; 13(5):2029-2035. PMC: 5443174. DOI: 10.3892/etm.2017.4183. View

Lu P, Jones L, Snyder E, Tuszynski M . Neural stem cells constitutively secrete neurotrophic factors and promote extensive host axonal growth after spinal cord injury. Exp Neurol. 2003; 181(2):115-29. DOI: 10.1016/s0014-4886(03)00037-2. View

Dai X, Liu L, Ouyang J, Li X, Zhang X, Lan Q . Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers. Sci Rep. 2017; 7(1):1457. PMC: 5431218. DOI: 10.1038/s41598-017-01581-y. View

Weidner N, Blesch A, Grill R, Tuszynski M . Nerve growth factor-hypersecreting Schwann cell grafts augment and guide spinal cord axonal growth and remyelinate central nervous system axons in a phenotypically appropriate manner that correlates with expression of L1. J Comp Neurol. 1999; 413(4):495-506. DOI: 10.1002/(sici)1096-9861(19991101)413:4<495::aid-cne1>3.0.co;2-z. View

10.

Ning L, Sun H, Lelong T, Guilloteau R, Zhu N, Schreyer D . 3D bioprinting of scaffolds with living Schwann cells for potential nerve tissue engineering applications. Biofabrication. 2018; 10(3):035014. DOI: 10.1088/1758-5090/aacd30. View

11.

Watabe K, Fukuda T, Tanaka J, Honda H, Toyohara K, Sakai O . Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine growth-promoting activities. J Neurosci Res. 1995; 41(2):279-90. DOI: 10.1002/jnr.490410215. View

12.

Owens C, Marga F, Forgacs G, Heesch C . Biofabrication and testing of a fully cellular nerve graft. Biofabrication. 2013; 5(4):045007. PMC: 4007150. DOI: 10.1088/1758-5082/5/4/045007. View

13.

Teng Y, Lavik E, Qu X, Park K, Ourednik J, Zurakowski D . Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells. Proc Natl Acad Sci U S A. 2002; 99(5):3024-9. PMC: 122466. DOI: 10.1073/pnas.052678899. View

14.

Xu X, Guenard V, Kleitman N, Bunge M . Axonal regeneration into Schwann cell-seeded guidance channels grafted into transected adult rat spinal cord. J Comp Neurol. 1995; 351(1):145-60. DOI: 10.1002/cne.903510113. View

15.

Chen G, Hu Y, Wan H, Xia L, Li J, Yang F . Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells and Schwann cells. Chin Med J (Engl). 2010; 123(17):2424-31. View

16.

Xu X, Chen A, Guenard V, Kleitman N, Bunge M . Bridging Schwann cell transplants promote axonal regeneration from both the rostral and caudal stumps of transected adult rat spinal cord. J Neurocytol. 1997; 26(1):1-16. DOI: 10.1023/a:1018557923309. View

17.

Tetzlaff W, Okon E, Karimi-Abdolrezaee S, Hill C, Sparling J, Plemel J . A systematic review of cellular transplantation therapies for spinal cord injury. J Neurotrauma. 2010; 28(8):1611-82. PMC: 3143488. DOI: 10.1089/neu.2009.1177. View

18.

Ogawa Y, Sawamoto K, Miyata T, MIYAO S, Watanabe M, Nakamura M . Transplantation of in vitro-expanded fetal neural progenitor cells results in neurogenesis and functional recovery after spinal cord contusion injury in adult rats. J Neurosci Res. 2002; 69(6):925-33. DOI: 10.1002/jnr.10341. View

19.

Terenghi G . Peripheral nerve regeneration and neurotrophic factors. J Anat. 1999; 194 ( Pt 1):1-14. PMC: 1467889. DOI: 10.1046/j.1469-7580.1999.19410001.x. View

20.

Horner P, Gage F . Regenerating the damaged central nervous system. Nature. 2000; 407(6807):963-70. DOI: 10.1038/35039559. View