Preserving Airway Smooth Muscle Contraction in Precision-Cut Lung Slices

Overview

Journal Sci Rep

Specialty Science

Date 2020 Apr 17

PMID 32296115

Citations 16

Authors

Guang Li

Jonathan A Cohen

Carolina Martines

Sumati Ram-Mohan

Joseph D Brain

Ramaswamy Krishnan

Xingbin Ai

Yan Bai

Affiliations

Soon will be listed here.

Abstract

Precision-cut lung slices (PCLS) are ideal for measuring small airway contraction. However, these measurements are currently limited to acute exposure scenarios that typically last a few minutes to a few hours. Using an insulin-supplemented culture medium, we prolong the small airway contractility in mouse PCLS for up to two weeks. Compared to conventional culture medium, insulin-supplemented culture medium provides no additional benefit in preserving cellular viability or airway structure. However, it protects the airway smooth muscle (ASM) against a loss of smooth muscle myosin heavy chain (SMMHC) expression. We elucidate the significance of this new culture medium for chronic disease modeling of IL-13-induced airway hyper-responsiveness.

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