Galectin-2 Has Bactericidal Effects Against in a β-galactoside-Dependent Manner

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Apr 17

PMID 32295066

Citations 6

Authors

Takaharu Sasaki

Rei Saito

Midori Oyama

Tomoharu Takeuchi

Toru Tanaka

Hideshi Natsume

Mayumi Tamura

Yoichiro Arata

Tomomi Hatanaka

Affiliations

Soon will be listed here.

Abstract

is associated with the onset of gastritis, peptic ulcers, and gastric cancer. Galectins are a family of β-galactoside-binding proteins involved in diverse biological phenomena. Galectin-2 (Gal-2), a member of the galectin family, is predominantly expressed in the gastrointestinal tract. Although some galectin family proteins are involved in immunoreaction, the role of Gal-2 against infection remains unclear. In this study, the effects of Gal-2 on morphology and survival were examined. Gal-2 induced aggregation depending on β-galactoside and demonstrated a bactericidal effect. Immunohistochemical staining of the gastric tissue indicated that Gal-2 existed in the gastric mucus, as well as mucosa. These results suggested that Gal-2 plays a role in innate immunity against infection in gastric mucus.

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References

Saal I, Nagy N, Lensch M, Lohr M, Manning J, Decaestecker C . Human galectin-2: expression profiling by RT-PCR/immunohistochemistry and its introduction as a histochemical tool for ligand localization. Histol Histopathol. 2005; 20(4):1191-208. DOI: 10.14670/HH-20.1191. View

Ahmed H, Fink N, Pohl J, Vasta G . Galectin-1 from bovine spleen: biochemical characterization, carbohydrate specificity and tissue-specific isoform profiles. J Biochem. 1996; 120(5):1007-19. DOI: 10.1093/oxfordjournals.jbchem.a021493. View

Takaishi S, Wang T . Gene expression profiling in a mouse model of Helicobacter-induced gastric cancer. Cancer Sci. 2007; 98(3):284-93. PMC: 11159662. DOI: 10.1111/j.1349-7006.2007.00392.x. View

Ota H, Katsuyama T . Alternating laminated array of two types of mucin in the human gastric surface mucous layer. Histochem J. 1992; 24(2):86-92. DOI: 10.1007/BF01082444. View

Estevam R, da Silva N, Wood da Silva , Fonseca F, Oliveira A, Nogueira . Modulation of Galectin-3 and Galectin 9 in gastric mucosa of patients with chronic gastritis and positive Helicobacter pylori infection. Pathol Res Pract. 2017; 213(10):1276-1281. DOI: 10.1016/j.prp.2017.08.005. View

Thung I, Aramin H, Vavinskaya V, Gupta S, Park J, Crowe S . Review article: the global emergence of Helicobacter pylori antibiotic resistance. Aliment Pharmacol Ther. 2015; 43(4):514-33. PMC: 5064663. DOI: 10.1111/apt.13497. View

Appelmelk B, Shiberu B, Trinks C, Tapsi N, Zheng P, Verboom T . Phase variation in Helicobacter pylori lipopolysaccharide. Infect Immun. 1998; 66(1):70-6. PMC: 107860. DOI: 10.1128/IAI.66.1.70-76.1998. View

Hirabayashi J, Hashidate T, Arata Y, Nishi N, Nakamura T, Hirashima M . Oligosaccharide specificity of galectins: a search by frontal affinity chromatography. Biochim Biophys Acta. 2002; 1572(2-3):232-54. DOI: 10.1016/s0304-4165(02)00311-2. View

Sakakura M, Tamura M, Fujii N, Takeuchi T, Hatanaka T, Kishimoto S . Structural mechanisms for the S-nitrosylation-derived protection of mouse galectin-2 from oxidation-induced inactivation revealed by NMR. FEBS J. 2018; 285(6):1129-1145. DOI: 10.1111/febs.14397. View

10.

Wang G, Ge Z, Rasko D, Taylor D . Lewis antigens in Helicobacter pylori: biosynthesis and phase variation. Mol Microbiol. 2000; 36(6):1187-96. DOI: 10.1046/j.1365-2958.2000.01934.x. View

11.

Pereira M, Medeiros J . Role of Helicobacter pylori in gastric mucosa-associated lymphoid tissue lymphomas. World J Gastroenterol. 2014; 20(3):684-98. PMC: 3921478. DOI: 10.3748/wjg.v20.i3.684. View

12.

Kasai K, Hirabayashi J . Galectins: a family of animal lectins that decipher glycocodes. J Biochem. 1996; 119(1):1-8. DOI: 10.1093/oxfordjournals.jbchem.a021192. View

13.

Park A, Hagiwara S, Hsu D, Liu F, Yoshie O . Galectin-3 Plays an Important Role in Innate Immunity to Gastric Infection by Helicobacter pylori. Infect Immun. 2016; 84(4):1184-1193. PMC: 4807496. DOI: 10.1128/IAI.01299-15. View

14.

Asaka M, Kato M, Sakamoto N . Roadmap to eliminate gastric cancer with Helicobacter pylori eradication and consecutive surveillance in Japan. J Gastroenterol. 2013; 49(1):1-8. PMC: 3895201. DOI: 10.1007/s00535-013-0897-8. View

15.

Ensgraber M, Loos M . A 66-kilodalton heat shock protein of Salmonella typhimurium is responsible for binding of the bacterium to intestinal mucus. Infect Immun. 1992; 60(8):3072-8. PMC: 257283. DOI: 10.1128/iai.60.8.3072-3078.1992. View

16.

Nishida T, Tsujii M, Tanimura H, Tsutsui S, Tsuji S, Takeda A . Comparative study of esomeprazole and lansoprazole in triple therapy for eradication of Helicobacter pylori in Japan. World J Gastroenterol. 2014; 20(15):4362-9. PMC: 3989972. DOI: 10.3748/wjg.v20.i15.4362. View

17.

Paclik D, Berndt U, Guzy C, Dankof A, Danese S, Holzloehner P . Galectin-2 induces apoptosis of lamina propria T lymphocytes and ameliorates acute and chronic experimental colitis in mice. J Mol Med (Berl). 2007; 86(12):1395-406. DOI: 10.1007/s00109-007-0290-2. View

18.

Price A, Levi J, Dolby J, Dunscombe P, Smith A, Clark J . Campylobacter pyloridis in peptic ulcer disease: microbiology, pathology, and scanning electron microscopy. Gut. 1985; 26(11):1183-8. PMC: 1432923. DOI: 10.1136/gut.26.11.1183. View

19.

Barrow H, Guo X, Wandall H, Pedersen J, Fu B, Zhao Q . Serum galectin-2, -4, and -8 are greatly increased in colon and breast cancer patients and promote cancer cell adhesion to blood vascular endothelium. Clin Cancer Res. 2011; 17(22):7035-46. DOI: 10.1158/1078-0432.CCR-11-1462. View

20.

Lobsanov Y, Gitt M, Leffler H, Barondes S, Rini J . X-ray crystal structure of the human dimeric S-Lac lectin, L-14-II, in complex with lactose at 2.9-A resolution. J Biol Chem. 1993; 268(36):27034-8. DOI: 10.2210/pdb1hlc/pdb. View