(Vell.) Stellfeld Alleviates Acute Pain and Inflammation: Potential Mechanisms of Action

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2020 Apr 16

PMID 32290371

Citations 2

Authors

Juliana Agostinho Lopes

Vinicius Peixoto Rodrigues

Marcelo Marucci Pereira Tangerina

Lucia Regina Machado da Rocha

Catarine Massucato Nishijima

Vania Vasti Alfieri Nunes

Luiz Fernando Rolim de Almeida

Wagner Vilegas

Adair Roberto Soares Dos Santos

Miriam Sannomiya

Clelia Akiko Hiruma-Lima

Affiliations

Soon will be listed here.

Abstract

(Vell.) Stellfeld (Fabaceae) known in Brazil as "jacaranda de espinho" or "espinheira santa nativa" is a medicinal plant commonly used in folk medicine to treat ulcers, cough and diarrhea. This study aimed to investigate the anti-inflammatory and antinociceptive effects of hydroalcoholic extracts from M. hirtum twig (HEMh) using experimental models of nociception through the involvement of transient receptor potential channels, acid-sensing ion channel (ASIC), nitrergic, opioidergic, glutamatergic, and supraspinal pathways. Our results revealed an antinociceptive effect of HEMh mediated by the opioidergic, L-arginine-nitric oxide and glutamate systems, as well as by interactions with TRPA1/ASIC channels. The anti-inflammatory effect of HEMh evaluated with a xylene-induced ear edema and by the involvement of arachidonic acid and prostaglandin E2 (PGE) showed involvement of the COX pathway, based on observed decreases in PGE levels. A phytochemical investigation of the HEMh led to the isolation of α-amyrin, β-amyrin, allantoin, apigenin-7-methoxy-6--β-D-glucopyranoside, and apigenin-6--β-D-glucopyranosyl-8--β-D-xylopyranoside. In conclusion, the acute oral administration of HEMh inhibits the nociceptive behavioral response in animals through the nitrergic, opioid, glutamatergic pathways, and by inhibition of the TRPA1 and ASIC channels, without causing locomotor dysfunction. In addition, its anti-inflammatory effect is associated with the COX pathway and decreased PGE levels.

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