Human Obesity Induces Dysfunction and Early Senescence in Adipose Tissue-Derived Mesenchymal Stromal/Stem Cells

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2020 Apr 11

PMID 32274385

Citations 60

Authors

Sabena M Conley

LaTonya J Hickson

Todd A Kellogg

Travis McKenzie

Julie K Heimbach

Timucin Taner

Hui Tang

Kyra L Jordan

Ishran M Saadiq

John R Woollard

Busra Isik

Mohsen Afarideh

Tamar Tchkonia

James L Kirkland

Lilach O Lerman

Affiliations

Soon will be listed here.

Abstract

Background: Chronic inflammatory conditions like obesity may adversely impact the biological functions underlying the regenerative potential of mesenchymal stromal/stem cells (MSC). Obesity can impair MSC function by inducing cellular senescence, a growth-arrest program that transitions cells to a pro-inflammatory state. However, the effect of obesity on adipose tissue-derived MSC in human subjects remains unclear. We tested the hypothesis that obesity induces senescence and dysfunction in human MSC.

Methods: MSC were harvested from abdominal subcutaneous fat collected from obese and age-matched non-obese subjects ( = 40) during bariatric or kidney donation surgeries, respectively. MSC were characterized, their migration and proliferation assessed, and cellular senescence evaluated by gene expression of cell-cycle arrest and senescence-associated secretory phenotype markers. studies tested MSC effect on injured human umbilical vein endothelial cells (HUVEC) function.

Results: Mean age was 59 ± 8 years, 66% were females. Obese subjects had higher body-mass index (BMI) than non-obese. MSC from obese subjects exhibited lower proliferative capacities than non-obese-MSC, suggesting decreased function, whereas their migration remained unchanged. Senescent cell burden and phenotype, manifested as , , , and gene expression, were significantly upregulated in obese subjects' MSC. BMI correlated directly with expression of , , and . Furthermore, co-incubation with non-obese, but not with obese-MSC, restored VEGF expression and tube formation that were blunted in injured HUVEC.

Conclusion: Human obesity triggers an early senescence program in adipose tissue-derived MSC. Thus, obesity-induced cellular injury may alter efficacy of this endogenous repair system and hamper the feasibility of autologous transplantation in obese individuals.

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