Stachyose Modulates Gut Microbiota and Alleviates Dextran Sulfate Sodium-induced Acute Colitis in Mice

Overview

Journal Saudi J Gastroenterol

Specialty Gastroenterology

Date 2020 Apr 10

PMID 32270772

Citations 16

Authors

Liwen He

Feiran Zhang

Zhengyang Jian

Jiachen Sun

Jiamin Chen

Vuekhang Liapao

Qing He

Affiliations

Soon will be listed here.

Abstract

Background/aim: Ulcerative colitis (UC) has been implicated to imbalanced enteric flora and reduced microbial diversity. Stachyose is a kind of natural prebiotic which favorably modulate the composition of the gut microbiota. The present study aims to investigate the effects of stachyose on inflammatory levels and gut microbiota of acute colitis mice.

Materials And Methods: In this study, the mice were randomly divided into four groups: (1) control group; (2) stachyose group; (3) dextran sulfate sodium (DSS) group; (4) stachyose + DSS group. Hemotoxylin and Eosin (H and E) staining was performed for the distal colon to examine the inflammation and tissue damage. The inflammatory cytokines including IL-6, IL-10, IL-17a, and TNF-α in serum were determined by ELISA assay. The differences in the gut microbiota were analyzed by 16S rDNA gene sequencing.

Results: Histological assay showed that the stachyose treatment significantly reduced the lesions of the colon in DSS-induced colitis. And the upregulated inflammatory cytokines induced by DSS were significantly inhibited by stachyose treatment. Additionally, the sequencing analysis showed that the stachyose changed the gut microbiota composition with a higher level of Akkermansia, as well as selectively increasing some probiotics, including Lactobacillus.

Conclusions: Our results suggested that stachyose increased beneficial microbiota and bacterial diversity to alleviate acute colitis in mice, which might be a new promising option to UC patients.

Citing Articles

Immunomodulatory effects of CCFM8661 + stachyose on cyclophosphamide-induced immunosuppression mice.

Ma W, Sun H, Lian L, Guo L, Wang Y, Huang L Front Immunol. 2025; 16:1513531.

PMID: 39931067 PMC: 11807972. DOI: 10.3389/fimmu.2025.1513531.

Two-pore channel regulators - Who is in control?.

Deutsch R, Kudrina V, Freichel M, Grimm C Front Physiol. 2025; 15:1534071.

PMID: 39867224 PMC: 11757267. DOI: 10.3389/fphys.2024.1534071.

The Combination of Host-Associated R32 and Stachyose Promotes the Intestinal Health of Turbot (. L).

Kong Y, Zhao S, Ou W, Mai K, Zhang Y Aquac Nutr. 2024; 2024:8658386.

PMID: 39555546 PMC: 11458268. DOI: 10.1155/2024/8658386.

Ulcerative colitis: molecular insights and intervention therapy.

Liang Y, Li Y, Lee C, Yu Z, Chen C, Liang C Mol Biomed. 2024; 5(1):42.

PMID: 39384730 PMC: 11464740. DOI: 10.1186/s43556-024-00207-w.

Identification of Yeast Strain YA176 for Bio-Purification of Soy Molasses to Produce Raffinose Family Oligosaccharides and Optimization of Fermentation Conditions.

Fu Z, Cheng S, Ma J, Basit R, Du Y, Tian S Appl Biochem Biotechnol. 2024; 197(2):943-963.

PMID: 39340630 DOI: 10.1007/s12010-024-05065-4.

References

Singla V, Chakkaravarthi S . Applications of prebiotics in food industry: A review. Food Sci Technol Int. 2017; 23(8):649-667. DOI: 10.1177/1082013217721769. View

Sellon R, Tonkonogy S, Schultz M, Dieleman L, Grenther W, Balish E . Resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice. Infect Immun. 1998; 66(11):5224-31. PMC: 108652. DOI: 10.1128/IAI.66.11.5224-5231.1998. View

Chassaing B, Aitken J, Malleshappa M, Vijay-Kumar M . Dextran sulfate sodium (DSS)-induced colitis in mice. Curr Protoc Immunol. 2014; 104:15.25.1-15.25.14. PMC: 3980572. DOI: 10.1002/0471142735.im1525s104. View

Jacob V, Crawford C, Cohen-Mekelburg S, Viladomiu M, Putzel G, Schneider Y . Single Delivery of High-Diversity Fecal Microbiota Preparation by Colonoscopy Is Safe and Effective in Increasing Microbial Diversity in Active Ulcerative Colitis. Inflamm Bowel Dis. 2017; 23(6):903-911. PMC: 6159890. DOI: 10.1097/MIB.0000000000001132. View

Chen L, Wang W, Zhou R, Ng S, Li J, Huang M . Characteristics of fecal and mucosa-associated microbiota in Chinese patients with inflammatory bowel disease. Medicine (Baltimore). 2014; 93(8):e51. PMC: 4602441. DOI: 10.1097/MD.0000000000000051. View

Ding X, Li Q, Li P, Zhang T, Cui B, Ji G . Long-Term Safety and Efficacy of Fecal Microbiota Transplant in Active Ulcerative Colitis. Drug Saf. 2019; 42(7):869-880. DOI: 10.1007/s40264-019-00809-2. View

McLean M, Dieguez Jr D, Miller L, Young H . Does the microbiota play a role in the pathogenesis of autoimmune diseases?. Gut. 2014; 64(2):332-41. PMC: 6288812. DOI: 10.1136/gutjnl-2014-308514. View

Yukawa T, Ohkusa T, Shibuya T, Tsukinaga S, Mitobe J, Takakura K . Nested culture method improves detection of Fusobacterium from stool in patients with ulcerative colitis. Jpn J Infect Dis. 2013; 66(2):109-14. DOI: 10.7883/yoken.66.109. View

Tyler A, Knox N, Kabakchiev B, Milgrom R, Kirsch R, Cohen Z . Characterization of the gut-associated microbiome in inflammatory pouch complications following ileal pouch-anal anastomosis. PLoS One. 2013; 8(9):e66934. PMC: 3782502. DOI: 10.1371/journal.pone.0066934. View

10.

Routy B, Le Chatelier E, Derosa L, Duong C, Alou M, Daillere R . Gut microbiome influences efficacy of PD-1-based immunotherapy against epithelial tumors. Science. 2017; 359(6371):91-97. DOI: 10.1126/science.aan3706. View

11.

Moayyedi P, Surette M, Kim P, Libertucci J, Wolfe M, Onischi C . Fecal Microbiota Transplantation Induces Remission in Patients With Active Ulcerative Colitis in a Randomized Controlled Trial. Gastroenterology. 2015; 149(1):102-109.e6. DOI: 10.1053/j.gastro.2015.04.001. View

12.

Batista D, Raffals L . Role of intestinal bacteria in the pathogenesis of pouchitis. Inflamm Bowel Dis. 2014; 20(8):1481-6. DOI: 10.1097/MIB.0000000000000055. View

13.

Carmen Collado M, Derrien M, Isolauri E, de Vos W, Salminen S . Intestinal integrity and Akkermansia muciniphila, a mucin-degrading member of the intestinal microbiota present in infants, adults, and the elderly. Appl Environ Microbiol. 2007; 73(23):7767-70. PMC: 2168041. DOI: 10.1128/AEM.01477-07. View

14.

Liying Z, Li D, Qiao S, Johnson E, Li B, Thacker P . Effects of stachyose on performance, diarrhoea incidence and intestinal bacteria in weanling pigs. Arch Tierernahr. 2003; 57(1):1-10. View

15.

Michail S, Durbin M, Turner D, Griffiths A, Mack D, Hyams J . Alterations in the gut microbiome of children with severe ulcerative colitis. Inflamm Bowel Dis. 2011; 18(10):1799-808. PMC: 3319508. DOI: 10.1002/ibd.22860. View

16.

Leclercq S, Matamoros S, Cani P, Neyrinck A, Jamar F, Starkel P . Intestinal permeability, gut-bacterial dysbiosis, and behavioral markers of alcohol-dependence severity. Proc Natl Acad Sci U S A. 2014; 111(42):E4485-93. PMC: 4210345. DOI: 10.1073/pnas.1415174111. View

17.

Macfarlane G, Cummings J . Probiotics and prebiotics: can regulating the activities of intestinal bacteria benefit health?. West J Med. 2008; 171(3):187-91. PMC: 1305806. View

18.

Reunanen J, Kainulainen V, Huuskonen L, Ottman N, Belzer C, Huhtinen H . Akkermansia muciniphila Adheres to Enterocytes and Strengthens the Integrity of the Epithelial Cell Layer. Appl Environ Microbiol. 2015; 81(11):3655-62. PMC: 4421065. DOI: 10.1128/AEM.04050-14. View

19.

Qian Y, Zhao X, Song J, Zhu K, Sun P, Li G . Inhibitory effects of resistant starch (RS3) as a carrier for stachyose on dextran sulfate sodium-induced ulcerative colitis in C57BL/6 mice. Exp Ther Med. 2013; 6(5):1312-1316. PMC: 3820662. DOI: 10.3892/etm.2013.1280. View

20.

Manichanh C, Rigottier-Gois L, Bonnaud E, Gloux K, Pelletier E, Frangeul L . Reduced diversity of faecal microbiota in Crohn's disease revealed by a metagenomic approach. Gut. 2005; 55(2):205-11. PMC: 1856500. DOI: 10.1136/gut.2005.073817. View