Neuroprotective Effect of Bergamot Juice in 6-OHDA-Induced SH-SY5Y Cell Death, an Model of Parkinson's Disease

Overview

Journal Pharmaceutics

Publisher MDPI

Date 2020 Apr 9

PMID 32260543

Citations 21

Authors

Nadia Ferlazzo

Santa Cirmi

Alessandro Maugeri

Caterina Russo

Giovanni Enrico Lombardo

Sebastiano Gangemi

Gioacchino Calapai

Vincenzo Mollace

Michele Navarra

Affiliations

Soon will be listed here.

Abstract

Much evidence suggests that both oxidative stress and apoptosis play a key role in the pathogenesis of Parkinson's disease (PD). The present study aims to evaluate the protective effect of bergamot juice (BJ) against 6-hydroxydopamine (6-OHDA)- or HO-induced cell death. Treatment of differentiated SH-SY5Y human neuroblastoma cells with 6-OHDA or HO resulted in cell death that was significantly reduced by the pre-treatment with BJ. The protective effects of BJ seem to correlate with the reduction of intracellular reactive oxygen species and nitric oxide generation caused by 6-OHDA or HO. BJ also attenuated mitochondrial dysfunction, caspase-3 activation, imbalance of pro- and anti-apoptotic proteins, MAPKs activation and reduced NF-ĸB nuclear translocation evoked by neurotoxic agents. Additionally, BJ exhibited excellent antioxidant capability in assays. Collectively, our results suggest that BJ exerts neuroprotective effect through the interplay with specific cell targets and its antioxidant activity, making it worthy of consideration for the management of neurodegenerative diseases.

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