MiR-145-5p Regulates the Proliferation, Migration and Invasion in Cervical Carcinoma by Targeting KLF5

Overview

Journal Onco Targets Ther

Publisher Dove Medical Press

Specialty Oncology

Date 2020 Apr 8

PMID 32256087

Citations 13

Authors

Hui Cao

Guihua Pan

Shiqiang Tang

Ni Zhong

Huake Liu

Haizhi Zhou

Qin Peng

Yongbin Zou

Affiliations

Soon will be listed here.

Abstract

Objective: Cervical carcinoma (CC) is a serious threat to women's health and few effective therapeutic methods have been discovered. The purpose of this study is to explore the underlying mechanism of miR-145-5p in CC.

Methods: Bioinformatics methods were employed to analyze the gene expression data of CC from TCGA database. qRT-PCR was used to detect the expression of miR-145-5p and KLF5 in CC cells, and Western blot was employed for the examination of KLF5 protein level. The targeted relationship between miR-145-5p and KLF5 was verified by a dual-luciferase reporter assay. Moreover, CCK-8, wound healing assay and transwell invasion assay were used to analyze the effects of miR-145-5p overexpression or KLF5 silencing on the proliferation, migration and invasion of CC cells.

Results: miR-145-5p was shown to be down-regulated in CC tissues and cells, while KLF5 was up-regulated. miR-145-5p could bind to the complementary sequence within the wild type KLF5 3'UTR rather than the mutant one. In addition, miR-145-5p could effectively down-regulate KLF5, in turn inhibiting the proliferation, migration and invasion of CC cells.

Conclusion: miR-145-5p regulates the proliferation, migration and invasion of CC cells by targeting KLF5.

Citing Articles

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References

Sathyanarayanan A, Chandrasekaran K, Karunagaran D . microRNA-145 modulates epithelial-mesenchymal transition and suppresses proliferation, migration and invasion by targeting SIP1 in human cervical cancer cells. Cell Oncol (Dordr). 2016; 40(2):119-131. DOI: 10.1007/s13402-016-0307-3. View

Liu J, Bian T, Feng J, Qian L, Zhang J, Jiang D . miR-335 inhibited cell proliferation of lung cancer cells by target Tra2β. Cancer Sci. 2017; 109(2):289-296. PMC: 5797811. DOI: 10.1111/cas.13452. View

Li M, Hu X . Meta-analysis of microRNA expression profiling studies in human cervical cancer. Med Oncol. 2015; 32(6):510. DOI: 10.1007/s12032-015-0510-5. View

Ma D, Chang L, Zhao S, Zhao J, Xiong Y, Cao F . KLF5 promotes cervical cancer proliferation, migration and invasion in a manner partly dependent on TNFRSF11a expression. Sci Rep. 2017; 7(1):15683. PMC: 5691198. DOI: 10.1038/s41598-017-15979-1. View

Rupaimoole R, Slack F . MicroRNA therapeutics: towards a new era for the management of cancer and other diseases. Nat Rev Drug Discov. 2017; 16(3):203-222. DOI: 10.1038/nrd.2016.246. View

. Cervical Cancer Analysis Reveals New Mutations. Cancer Discov. 2017; 7(4):344. DOI: 10.1158/2159-8290.CD-NB2017-020. View

Schiffman M, Castle P, Jeronimo J, Rodriguez A, Wacholder S . Human papillomavirus and cervical cancer. Lancet. 2007; 370(9590):890-907. DOI: 10.1016/S0140-6736(07)61416-0. View

Ma L, Li L . miR-145 Contributes to the Progression of Cervical Carcinoma by Directly Regulating FSCN1. Cell Transplant. 2019; 28(9-10):1299-1305. PMC: 6767894. DOI: 10.1177/0963689719861063. View

Dizon D, MacKay H, Thomas G, Werner T, Kohn E, Hess D . State of the science in cervical cancer: where we are today and where we need to go. Cancer. 2014; 120(15):2282-8. DOI: 10.1002/cncr.28722. View

10.

Ye C, Sun N, Ma Y, Zhao Q, Zhang Q, Xu C . MicroRNA-145 contributes to enhancing radiosensitivity of cervical cancer cells. FEBS Lett. 2015; 589(6):702-9. DOI: 10.1016/j.febslet.2015.01.037. View

11.

Gonzalez-Torres A, Banuelos-Villegas E, Martinez-Acuna N, Sulpice E, Gidrol X, Alvarez-Salas L . MYPT1 is targeted by miR-145 inhibiting viability, migration and invasion in 2D and 3D HeLa cultures. Biochem Biophys Res Commun. 2018; 507(1-4):348-354. DOI: 10.1016/j.bbrc.2018.11.039. View

12.

Lei C, Du F, Sun L, Li T, Li T, Min Y . miR-143 and miR-145 inhibit gastric cancer cell migration and metastasis by suppressing MYO6. Cell Death Dis. 2017; 8(10):e3101. PMC: 5682659. DOI: 10.1038/cddis.2017.493. View

13.

Ghaleb A, Nandan M, Chanchevalap S, Dalton W, Hisamuddin I, Yang V . Krüppel-like factors 4 and 5: the yin and yang regulators of cellular proliferation. Cell Res. 2005; 15(2):92-6. PMC: 1317089. DOI: 10.1038/sj.cr.7290271. View

14.

Zhang H, Lu Y, Wang S, Sheng X, Zhang S . MicroRNA-152 Acts as a Tumor Suppressor MicroRNA by Inhibiting Krüppel-Like Factor 5 in Human Cervical Cancer. Oncol Res. 2018; 27(3):335-340. PMC: 7848453. DOI: 10.3727/096504018X15252202178408. View

15.

Wei H, Wen-Ming C, Jun-Bo J . Plasma miR-145 as a novel biomarker for the diagnosis and radiosensitivity prediction of human cervical cancer. J Int Med Res. 2017; 45(3):1054-1060. PMC: 5536414. DOI: 10.1177/0300060517709614. View

16.

Shi M, Du L, Liu D, Qian L, Hu M, Yu M . Glucocorticoid regulation of a novel HPV-E6-p53-miR-145 pathway modulates invasion and therapy resistance of cervical cancer cells. J Pathol. 2012; 228(2):148-57. DOI: 10.1002/path.3997. View

17.

Huang L, Lin J, Yu Y, Zhang M, Wang H, Zheng M . Downregulation of six microRNAs is associated with advanced stage, lymph node metastasis and poor prognosis in small cell carcinoma of the cervix. PLoS One. 2012; 7(3):e33762. PMC: 3306296. DOI: 10.1371/journal.pone.0033762. View

18.

Joura E, Giuliano A, Iversen O, Bouchard C, Mao C, Mehlsen J . A 9-valent HPV vaccine against infection and intraepithelial neoplasia in women. N Engl J Med. 2015; 372(8):711-23. DOI: 10.1056/NEJMoa1405044. View

19.

Li J, Chu Z, Han H, Zhang Y, Tian F, Zhang J . Suppression of miR-93-5p inhibits high-risk HPV-positive cervical cancer progression via targeting of BTG3. Hum Cell. 2019; 32(2):160-171. DOI: 10.1007/s13577-018-00225-1. View

20.

Berindan-Neagoe I, Del C Monroig P, Pasculli B, Calin G . MicroRNAome genome: a treasure for cancer diagnosis and therapy. CA Cancer J Clin. 2014; 64(5):311-36. PMC: 4461198. DOI: 10.3322/caac.21244. View