Long Non-coding RNA TPT1-AS1 Promotes Angiogenesis and Metastasis of Colorectal Cancer Through TPT1-AS1/NF90/VEGFA Signaling Pathway

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2020 Apr 6

PMID 32248186

Citations 25

Authors

Yiyun Zhang

Jiangyun Sun

Yuan Qi

Yimin Wang

Yu Ding

Kun Wang

Qingxin Zhou

Jingxuan Wang

Fei Ma

Jianguo Zhang

Baoliang Guo

Affiliations

Soon will be listed here.

Abstract

LncRNAs have been proven closely correlated to tumor progression. A recent study identified LncRNA TPT1-AS1 (TPT1-AS1) as one of the liver-metastasis associated LncRNAs in colorectal cancer (CRC). In this study, we report that TPT1-AS1 is upregulated in CRC tissues, which is associated with poor prognosis. Functional assays unravel a pro-angiogenesis and metastasis role of TPT1-AS1. Mechanistically, Flexmap 3D assays reveal that TPT1-AS1 upregulates the VEGFA secretion in CRC cells. RNA immunoprecipitation and mRNA stability assays further show that TPT1-AS1 interacts with nuclear factor 90 (NF90) and subsequently promotes the association between NF90 and VEGFA mRNA, which leads to the upregulation of VEGFA mRNA stability. Therefore, we elucidate a new regulatory mechanism of TPT1-AS1 in CRC angiogenesis and targeting the TPT1-AS1/NF90/VEGFA axis may provide a useful strategy for diagnosis and treatment for colorectal cancer patients.

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