Secretory Functions of Macrophages in the Human Pancreatic Islet Are Regulated by Endogenous Purinergic Signaling

Overview

Journal Diabetes

Specialty Endocrinology

Date 2020 Apr 5

PMID 32245801

Citations 22

Authors

Jonathan R Weitz

Carol Jacques-Silva

Mirza Muhammed Fahd Qadir

Oliver Umland

Elizabeth Pereira

Farhan Qureshi

Alejandro Tamayo

Juan Dominguez-Bendala

Rayner Rodriguez-Diaz

Joana Almaca

Alejandro Caicedo

Affiliations

Soon will be listed here.

Abstract

Endocrine cells of the pancreatic islet interact with their microenvironment to maintain tissue homeostasis. Communication with local macrophages is particularly important in this context, but the homeostatic functions of human islet macrophages are not known. In this study, we show that the human islet contains macrophages in perivascular regions that are the main local source of the anti-inflammatory cytokine interleukin-10 (IL-10) and the metalloproteinase MMP9. Macrophage production and secretion of these homeostatic factors are controlled by endogenous purinergic signals. In obese and diabetic states, macrophage expression of purinergic receptors MMP9 and IL-10 is reduced. We propose that in those states, exacerbated β-cell activity due to increased insulin demand and increased cell death produce high levels of ATP that downregulate purinergic receptor expression. Loss of ATP sensing in macrophages may reduce their secretory capacity.

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