Role of in the Molting and Metamorphosis of the Cigarette Beetle

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Apr 5

PMID 32244803

Citations 9

Authors

Wen-Jia Yang

Kang-Kang Xu

Yi Yan

Can Li

Dao-Chao Jin

Affiliations

Soon will be listed here.

Abstract

Chitin deacetylases (CDAs) are chitin-modifying enzymes known to play vital roles in insect metamorphosis and development. In this study, we identified and characterized a gene () from the cigarette beetle . contains a 1614 bp open reading frame encoding a protein of 537 amino acids that includes domain structures typical of CDAs. was mainly expressed in the late larval and late pupal stages. In larval tissues, the highest level of was detected in the integument. The expression of was induced by 20-hydroxyecdysone (20E) in vivo, and it was significantly suppressed by knocking down the expression of ecdysteroidogenesis genes and 20E signaling genes. RNA interference (RNAi)-aided silencing of in fifth-instar larvae prevented the larval-pupal molt and caused 75% larval mortality. In the late pupal stage, depletion of resulted in the inhibition of pupal growth and wing abnormalities, and the expression levels of four wing development-related genes (DY, , , and ) were dramatically decreased. Meanwhile, the chitin contents of RNAi beetles were significantly reduced, and expressions of three chitin synthesis pathway genes (, , and ) were greatly decreased. The results suggest that is indispensable for larval-pupal and pupal-adult molts, and that it is a potential target for the RNAi-based control of .

Citing Articles

Roles of Vitellogenin and Its Receptor Genes in Female Reproduction of the Cigarette Beetle, .

Guo Q, Zu M, Liu D, Yan Y, Yang W, Xu K Insects. 2025; 16(2).

PMID: 40003805 PMC: 11857020. DOI: 10.3390/insects16020175.

Knockdown of and genes increases the susceptibility of to ethyl formate and benzothiazole.

Li X, Ma L, Yang W, Xu K Front Physiol. 2024; 15:1503953.

PMID: 39633644 PMC: 11615064. DOI: 10.3389/fphys.2024.1503953.

Gene as an Optimal RNAi-Based Target for Controlling the Tomato Leaf Miner .

Zhou Y, Zhang Y, Xu K, Liu R, Liu W, Ma H Insects. 2024; 15(11).

PMID: 39590437 PMC: 11595049. DOI: 10.3390/insects15110838.

Identification and Functional Insights of Knickkopf Genes in the Larval Cuticle of .

Zeng M, Zhou W, Wen S, Wu H, Zhang Q, Fu K Insects. 2024; 15(8).

PMID: 39194827 PMC: 11354708. DOI: 10.3390/insects15080623.

Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, .

Wu H, Zhao D, Guo X, Liu Z, Li R, Lu X Int J Mol Sci. 2023; 24(4).

PMID: 36834492 PMC: 9961250. DOI: 10.3390/ijms24043076.

References

Toprak U, Baldwin D, Erlandson M, Gillott C, Hou X, Coutu C . A chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix. Insect Mol Biol. 2008; 17(5):573-85. DOI: 10.1111/j.1365-2583.2008.00829.x. View

Lyu Z, Chen J, Li Z, Cheng J, Wang C, Lin T . Knockdown of β-N-acetylglucosaminidase gene disrupts molting process in Heortia vitessoides Moore. Arch Insect Biochem Physiol. 2019; 101(4):e21561. DOI: 10.1002/arch.21561. View

Moussian B, Schwarz H, Bartoszewski S, Nusslein-Volhard C . Involvement of chitin in exoskeleton morphogenesis in Drosophila melanogaster. J Morphol. 2005; 264(1):117-30. DOI: 10.1002/jmor.10324. View

Yang W, Chen C, Yan Y, Xu K, Li C . Clip-Domain Serine Protease Gene (CLIP3) Is Essential for Larval-Pupal Molting and Immunity in . Front Physiol. 2020; 10:1631. PMC: 7005593. DOI: 10.3389/fphys.2019.01631. View

Yang W, Xu K, Cong L, Wang J . Identification, mRNA expression, and functional analysis of chitin synthase 1 gene and its two alternative splicing variants in oriental fruit fly, Bactrocera dorsalis. Int J Biol Sci. 2013; 9(4):331-42. PMC: 3619095. DOI: 10.7150/ijbs.6022. View

Zhuo W, Fang Y, Kong L, Li X, Sima Y, Xu S . Chitin synthase A: a novel epidermal development regulation gene in the larvae of Bombyx mori. Mol Biol Rep. 2014; 41(7):4177-86. DOI: 10.1007/s11033-014-3288-1. View

Yang W, Xu K, Cao Y, Meng Y, Liu Y, Li C . Identification and Expression Analysis of Four Small Heat Shock Protein Genes in Cigarette Beetle, (Fabricius). Insects. 2019; 10(5). PMC: 6572347. DOI: 10.3390/insects10050139. View

Yang W, Xu K, Yan X, Li C . Knockdown of --acetylglucosaminidase 2 Impairs Molting and Wing Development in (Fabricius). Insects. 2019; 10(11). PMC: 6921043. DOI: 10.3390/insects10110396. View

Hironaka M, Kamura T, Osada M, Sasaki R, Shinoda K, Hariyama T . Adults of Lasioderma serricorne and Stegobium paniceum (Anobiidae: Coleoptera) Are Attracted to Ultraviolet (UV) Over Blue Light LEDs. J Econ Entomol. 2017; 110(4):1911-1915. DOI: 10.1093/jee/tox127. View

10.

Wang S, Jayaram S, Hemphala J, Senti K, Tsarouhas V, Jin H . Septate-junction-dependent luminal deposition of chitin deacetylases restricts tube elongation in the Drosophila trachea. Curr Biol. 2006; 16(2):180-5. DOI: 10.1016/j.cub.2005.11.074. View

11.

Clark-Hachtel C, Linz D, Tomoyasu Y . Insights into insect wing origin provided by functional analysis of vestigial in the red flour beetle, Tribolium castaneum. Proc Natl Acad Sci U S A. 2013; 110(42):16951-6. PMC: 3801059. DOI: 10.1073/pnas.1304332110. View

12.

Yan X, Zhao D, Zhang Y, Guo W, Wang W, Zhao K . Identification and characterization of chitin deacetylase2 from the American white moth, Hyphantria cunea (Drury). Gene. 2018; 670:98-105. DOI: 10.1016/j.gene.2018.05.069. View

13.

Bustin S, Benes V, Garson J, Hellemans J, Huggett J, Kubista M . The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009; 55(4):611-22. DOI: 10.1373/clinchem.2008.112797. View

14.

LARKIN M, Blackshields G, Brown N, Chenna R, McGettigan P, McWilliam H . Clustal W and Clustal X version 2.0. Bioinformatics. 2007; 23(21):2947-8. DOI: 10.1093/bioinformatics/btm404. View

15.

Xi Y, Pan P, Ye Y, Yu B, Zhang C . Chitin deacetylase family genes in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae). Insect Mol Biol. 2014; 23(6):695-705. DOI: 10.1111/imb.12113. View

16.

Tsigos I, Martinou A, Kafetzopoulos D, Bouriotis V . Chitin deacetylases: new, versatile tools in biotechnology. Trends Biotechnol. 2000; 18(7):305-12. DOI: 10.1016/s0167-7799(00)01462-1. View

17.

Shi J, Mu L, Guo W, Li G . IDENTIFICATION AND HORMONE INDUCTION OF PUTATIVE CHITIN SYNTHASE GENES AND SPLICE VARIANTS IN Leptinotarsa decemlineata (SAY). Arch Insect Biochem Physiol. 2016; 92(4):242-58. DOI: 10.1002/arch.21331. View

18.

Yao Q, Zhang D, Tang B, Chen J, Chen J, Lu L . Identification of 20-hydroxyecdysone late-response genes in the chitin biosynthesis pathway. PLoS One. 2010; 5(11):e14058. PMC: 2987807. DOI: 10.1371/journal.pone.0014058. View

19.

Guo W, Li G, Pang Y, Wang P . A novel chitin-binding protein identified from the peritrophic membrane of the cabbage looper, Trichoplusia ni. Insect Biochem Mol Biol. 2005; 35(11):1224-34. DOI: 10.1016/j.ibmb.2005.06.003. View

20.

Zhong X, Wang X, Tan X, Xia Q, Xiang Z, Zhao P . Identification and molecular characterization of a chitin deacetylase from Bombyx mori peritrophic membrane. Int J Mol Sci. 2014; 15(2):1946-61. PMC: 3958831. DOI: 10.3390/ijms15021946. View