The Core-Clock Gene Impacts Cell Motility In Vitro and Invasiveness in A Zebrafish Xenograft Colon Cancer Model

Overview

Journal Cancers (Basel)

Publisher MDPI

Specialty Oncology

Date 2020 Apr 5

PMID 32244760

Citations 14

Authors

Alireza Basti

Rita Fior

Muge Yalin

Vanda Povoa

Rosario Astaburuaga

Yin Li

Julian Naderi

Miguel Godinho Ferreira

Angela Relogio

Affiliations

Soon will be listed here.

Abstract

Malfunctions of circadian clock trigger abnormal cellular processes and influence tumorigenesis. Using an and xenograft model, we show that circadian clock disruption via the downregulation of the core-clock genes , , and impacts the circadian phenotype of , , and , and affects proliferation, apoptosis, and cell migration. In particular, both our and results suggest an impairment of cell motility and a reduction in micrometastasis formation upon knockdown of , accompanied by altered expression levels of and . Interestingly we show that differential proliferation and reduced tumour growth may be due to the additional influence of the host-clock and/or to the 3D tumour architecture. Our results raise new questions concerning host-tumour interaction and show that core-clock genes are involved in key cancer properties, including the regulation of cell migration and invasion by in zebrafish xenografts.

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