Molybdenum and Vanadium Nitrogenases of Azotobacter Chroococcum. Low Temperature Favours N2 Reduction by Vanadium Nitrogenase

Overview

Journal Biochem J

Specialty Biochemistry

Date 1988 Dec 1

PMID 3223922

Citations 31

Authors

R W Miller

R R Eady

Affiliations

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Abstract

A comparison of the effect of temperature on the reduction of N2 by purified molybdenum nitrogenase and vanadium nitrogenase of Azotobacter chroococcum showed differences in behaviour. As the assay temperature was lowered from 30 degrees C to 5 degrees C N2 remained an effective substrate for V nitrogenase, but not Mo nitrogenase, since the specific activity for N2 reduction by Mo nitrogenase decreased 10-fold more than that of V nitrogenase. Activity cross-reactions between nitrogenase components showed the enhanced low-temperature activity to be associated with the Fe protein of V nitrogenase. The lower activity of homologous Mo nitrogenase components, although dependent on the ratio of MoFe protein to Fe protein, did not equal that of V nitrogenase even under conditions of high electron flux obtained at a 12-fold molar excess of Fe protein.

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References

Dilworth M, Eady R, Eldridge M . The vanadium nitrogenase of Azotobacter chroococcum. Reduction of acetylene and ethylene to ethane. Biochem J. 1988; 249(3):745-51. PMC: 1148769. DOI: 10.1042/bj2490745. View

Eady R, Robson R, Richardson T, Miller R, Hawkins M . The vanadium nitrogenase of Azotobacter chroococcum. Purification and properties of the VFe protein. Biochem J. 1987; 244(1):197-207. PMC: 1147972. DOI: 10.1042/bj2440197. View

Hales B, Case E, Morningstar J, Dzeda M, Mauterer L . Isolation of a new vanadium-containing nitrogenase from Azotobacter vinelandii. Biochemistry. 1986; 25(23):7251-5. DOI: 10.1021/bi00371a001. View

Bishop P, Jarlenski D, Hetherington D . Evidence for an alternative nitrogen fixation system in Azotobacter vinelandii. Proc Natl Acad Sci U S A. 1980; 77(12):7342-6. PMC: 350499. DOI: 10.1073/pnas.77.12.7342. View

Hales B, Langosch D, Case E . Isolation and characterization of a second nitrogenase Fe-protein from Azotobacter vinelandii. J Biol Chem. 1986; 261(32):15301-6. View

Eady R, Smith B, Cook K, POSTGATE J . Nitrogenase of Klebsiella pneumoniae. Purification and properties of the component proteins. Biochem J. 1972; 128(3):655-75. PMC: 1173817. DOI: 10.1042/bj1280655. View

Bergstrom J, Eady R, Thorneley R . The vanadium- and molybdenum-containing nitrogenases of Azotobacter chroococcum. Comparison of mid-point potentials and kinetics of reduction by sodium dithionite of the iron proteins with bound magnesium adenosine 5'-diphosphate. Biochem J. 1988; 251(1):165-9. PMC: 1148978. DOI: 10.1042/bj2510165. View

Simpson F, BURRIS R . A nitrogen pressure of 50 atmospheres does not prevent evolution of hydrogen by nitrogenase. Science. 1984; 224(4653):1095-7. DOI: 10.1126/science.6585956. View

Watt G, Burns A . Kinetics of dithionite ion utilization and ATP hydrolysis for reactions catalyzed by the nitrogenase complex from Azotobacter vinelandii. Biochemistry. 1977; 16(2):264-70. DOI: 10.1021/bi00621a017. View

10.

Eady R, POSTGATE J . Nitrogenase. Nature. 1974; 249(460):805-10. DOI: 10.1038/249805a0. View

11.

Thorneley R, Lowe D . Nitrogenase of Klebsiella pneumoniae. Kinetics of the dissociation of oxidized iron protein from molybdenum-iron protein: identification of the rate-limiting step for substrate reduction. Biochem J. 1983; 215(2):393-403. PMC: 1152408. DOI: 10.1042/bj2150393. View

12.

Bishop P, Jarlenski D, Hetherington D . Expression of an alternative nitrogen fixation system in Azotobacter vinelandii. J Bacteriol. 1982; 150(3):1244-51. PMC: 216346. DOI: 10.1128/jb.150.3.1244-1251.1982. View

13.

Thorneley R, Eady R . Nitrogenase of Klebsiella pneumoniae. Distinction between proton-reducing and acetylene-reducing forms of the enzyme: effect of temperature and component protein ratio on substrate-reduction kinetics. Biochem J. 1977; 167(2):457-61. PMC: 1183677. DOI: 10.1042/bj1670457. View

14.

Robson R, Woodley P, Jones R . Second gene (nifH*) coding for a nitrogenase iron protein in Azotobacter chroococcum is adjacent to a gene coding for a ferredoxin-like protein. EMBO J. 1986; 5(6):1159-63. PMC: 1166922. DOI: 10.1002/j.1460-2075.1986.tb04341.x. View

15.

Eady R, Richardson T, Miller R, Hawkins M, Lowe D . The vanadium nitrogenase of Azotobacter chroococcum. Purification and properties of the Fe protein. Biochem J. 1988; 256(1):189-96. PMC: 1135386. DOI: 10.1042/bj2560189. View

16.

Corbin J . Liquid chromatographic-fluorescence determination of ammonia from nitrogenase reactions: a 2-min assay. Appl Environ Microbiol. 1984; 47(5):1027-30. PMC: 240047. DOI: 10.1128/aem.47.5.1027-1030.1984. View

17.

Yates M, Planque K . Nitrogenase from Azotobacter chroococcum. Purification and properties of the component proteins. Eur J Biochem. 1975; 60(2):467-76. DOI: 10.1111/j.1432-1033.1975.tb21025.x. View