First Evidence Showing That Pepper Vein Yellows Virus P4 Protein is a Movement Protein

Overview

Journal BMC Microbiol

Publisher Biomed Central

Specialty Microbiology

Date 2020 Apr 2

PMID 32228456

Citations 3

Authors

Sangsang Li

Xianyan Su

Xiangwen Luo

Yu Zhang

Deyong Zhang

Jiao Du

Zhanhong Zhang

Xian OuYang

Songbai Zhang

Yong Liu

Affiliations

Soon will be listed here.

Abstract

Background: Plant viruses move through plasmodesmata (PD) to infect new cells. To overcome the PD barrier, plant viruses have developed specific protein(s) to guide their genomic RNAs or DNAs to path through the PD.

Results: In the present study, we analyzed the function of Pepper vein yellows virus P4 protein. Our bioinformatic analysis using five commonly used algorithms showed that the P4 protein contains an transmembrane domain, encompassing the amino acid residue 117-138. The subcellular localization of P4 protein was found to target PD and form small punctates near walls. The P4 deletion mutant or the substitution mutant constructed by overlap PCR lost their function to produce punctates near the walls inside the fluorescent loci. The P4-YFP fusion was found to move from cell to cell in infiltrated leaves, and P4 could complement Cucumber mosaic virus movement protein deficiency mutant to move between cells.

Conclusion: Taking together, we consider that the P4 protein is a movement protein of Pepper vein yellows virus.

Citing Articles

Genome Editing for Plasmodesmal Biology.

Iswanto A, Shelake R, Vu M, Kim J, Kim S Front Plant Sci. 2021; 12:679140.

PMID: 34149780 PMC: 8207191. DOI: 10.3389/fpls.2021.679140.

Variability, Functions and Interactions of Plant Virus Movement Proteins: What Do We Know So Far?.

Kumar G, Dasgupta I Microorganisms. 2021; 9(4).

PMID: 33801711 PMC: 8066623. DOI: 10.3390/microorganisms9040695.

Small hydrophobic viral proteins involved in intercellular movement of diverse plant virus genomes.

Morozov S, Solovyev A AIMS Microbiol. 2020; 6(3):305-329.

PMID: 33134746 PMC: 7595835. DOI: 10.3934/microbiol.2020019.

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