Isolation and Characterisation of Alongshan Virus in Russia

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2020 Apr 1

PMID 32224888

Citations 35

Authors

Ivan S Kholodilov

Alexander G Litov

Alexander S Klimentov

Oxana A Belova

Alexandra E Polienko

Nikolai A Nikitin

Alexey M Shchetinin

Anna Y Ivannikova

Lesley Bell-Sakyi

Alexander S Yakovlev

Sergey V Bugmyrin

Liubov A Bespyatova

Larissa V Gmyl

Svetlana V Luchinina

Anatoly P Gmyl

Vladimir A Gushchin

Galina G Karganova

Affiliations

Soon will be listed here.

Abstract

In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmented ssRNA(+) genome. We detected Alongshan virus (ALSV), which is a representative of the JMV group, in ten pools of adult ticks collected in two geographically-separated Russian regions. Three of the ten strains were isolated in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most ALSV virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream open reading frame in the glycoprotein-coding segment of ALSV and other members of the JMV group.

Citing Articles

The segmented flavivirus Alongshan virus reduces mitochondrial mass by degrading STAT2 to suppress the innate immune response.

Zhao Y, Sui L, Pan M, Jin F, Huang Y, Fang S J Virol. 2024; 99(1):e0130124.

PMID: 39655955 PMC: 11784234. DOI: 10.1128/jvi.01301-24.

Epidemiology and global spread of emerging tick-borne Alongshan virus.

Gomer A, Lang A, Janshoff S, Steinmann J, Steinmann E Emerg Microbes Infect. 2024; 13(1):2404271.

PMID: 39259276 PMC: 11423535. DOI: 10.1080/22221751.2024.2404271.

Alongshan Virus Infection in Rangifer tarandus Reindeer, Northeastern China.

Xu W, Wang W, Li L, Li N, Liu Z, Che L Emerg Infect Dis. 2024; 30(7):1434-1437.

PMID: 38916639 PMC: 11210657. DOI: 10.3201/eid3007.231219.

Metatranscriptomic investigation of single ticks reveals diverse microbes, viruses, and novel mRNA-like endogenous viral elements.

Martyn C, Hayes B, Lauko D, Midthun E, Castaneda G, Bosco-Lauth A mSystems. 2024; 9(6):e0032124.

PMID: 38742892 PMC: 11237458. DOI: 10.1128/msystems.00321-24.

High-Throughput Sequencing Reveals Three Rhabdoviruses Persisting in the IRE/CTVM19 Cell Line.

Litov A, Shchetinin A, Kholodilov I, Belova O, Gadzhikurbanov M, Ivannikova A Viruses. 2024; 16(4).

PMID: 38675918 PMC: 11054507. DOI: 10.3390/v16040576.

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