TRIM32 Cooperates with Glycolytic Enzymes to Promote Cell Growth

Overview

Journal Elife

Specialty Biology

Date 2020 Apr 1

PMID 32223900

Citations 23

Authors

Simranjot Bawa

David S Brooks

Kathryn E Neville

Marla Tipping

Md Abdul Sagar

Joseph A Kollhoff

Geetanjali Chawla

Brian V Geisbrecht

Jason M Tennessen

Kevin W Eliceiri

Erika R Geisbrecht

Affiliations

Soon will be listed here.

Abstract

Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactions with the glycolytic enzymes Aldolase and Phosphoglycerate mutase. Loss of TRIM32, encoded by , shows reduced levels of glycolytic intermediates and amino acids. This altered metabolic profile correlates with a reduction in the size of glycolytic larval muscle and brain tissue. Consistent with a role for metabolic intermediates in glycolysis-driven biomass production, dietary amino acid supplementation in mutants improves muscle mass. Remarkably, TRIM32 is also required for ectopic growth - loss of TRIM32 in a wing disc-associated tumor model reduces glycolytic metabolism and restricts growth. Overall, our results reveal a novel role for TRIM32 for controlling glycolysis in the context of both normal development and tumor growth.

Citing Articles

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