Identification and Validation of Internal Reference Genes for Real-time Quantitative Polymerase Chain Reaction-based Studies in Hyalomma Anatolicum Ticks

Overview

Journal Ticks Tick Borne Dis

Publisher Elsevier

Date 2020 Mar 31

PMID 32222359

Citations 3

Authors

Cristiano Salata

Vanessa Monteil

Mikael Leijon

Lesley Bell-Sakyi

Ali Mirazimi

Affiliations

Soon will be listed here.

Abstract

Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne viral disease caused by the orthonairovirus CCHF virus (CCHFV). Ticks of the genus Hyalomma are the viral reservoir and they represent the main vector transmitting the virus to their hosts during blood feeding. However, how CCHFV replicates in its natural arthropod host cells and the nature of virus/host interactions are still largely unknown. With the aim of developing tools for use in this field, we identified and validated expression of four candidate endogenous control tick genes in a Hyalomma anatolicum-derived cell line. These genes will be useful for normalization of viral/cellular transcripts in infection/expression studies or as internal controls in molecular epidemiology surveys of pathogens transmitted by Hyalomma ticks.

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References

Salata C, Monteil V, Karlberg H, Celestino M, Devignot S, Leijon M . The DEVD motif of Crimean-Congo hemorrhagic fever virus nucleoprotein is essential for viral replication in tick cells. Emerg Microbes Infect. 2018; 7(1):190. PMC: 6258742. DOI: 10.1038/s41426-018-0192-0. View

Akinci E, Bodur H, Leblebicioglu H . Pathogenesis of Crimean-Congo hemorrhagic fever. Vector Borne Zoonotic Dis. 2013; 13(7):429-37. DOI: 10.1089/vbz.2012.1061. View

Jongejan F, Uilenberg G . The global importance of ticks. Parasitology. 2005; 129 Suppl:S3-14. DOI: 10.1017/s0031182004005967. View

Bell-Sakyi L . Continuous cell lines from the tick Hyalomma anatolicum anatolicum. J Parasitol. 1991; 77(6):1006-8. View

Koci J, Simo L, Park Y . Validation of internal reference genes for real-time quantitative polymerase chain reaction studies in the tick, Ixodes scapularis (Acari: Ixodidae). J Med Entomol. 2013; 50(1):79-84. PMC: 3703510. DOI: 10.1603/me12034. View

Bell-Sakyi L, Kohl A, Bente D, Fazakerley J . Tick cell lines for study of Crimean-Congo hemorrhagic fever virus and other arboviruses. Vector Borne Zoonotic Dis. 2011; 12(9):769-81. PMC: 3438810. DOI: 10.1089/vbz.2011.0766. View

Papa A, Mirazimi A, Koksal I, Estrada-Pena A, Feldmann H . Recent advances in research on Crimean-Congo hemorrhagic fever. J Clin Virol. 2014; 64:137-43. PMC: 4346445. DOI: 10.1016/j.jcv.2014.08.029. View

Spengler J, Bente D, Bray M, Burt F, Hewson R, Korukluoglu G . Second International Conference on Crimean-Congo Hemorrhagic Fever. Antiviral Res. 2017; 150:137-147. PMC: 6497152. DOI: 10.1016/j.antiviral.2017.11.019. View

Silver N, Best S, Jiang J, Thein S . Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol. 2006; 7:33. PMC: 1609175. DOI: 10.1186/1471-2199-7-33. View

10.

Gargili A, Estrada-Pena A, Spengler J, Lukashev A, Nuttall P, Bente D . The role of ticks in the maintenance and transmission of Crimean-Congo hemorrhagic fever virus: A review of published field and laboratory studies. Antiviral Res. 2017; 144:93-119. PMC: 6047067. DOI: 10.1016/j.antiviral.2017.05.010. View

11.

Bell-Sakyi L, Darby A, Baylis M, Makepeace B . The Tick Cell Biobank: A global resource for in vitro research on ticks, other arthropods and the pathogens they transmit. Ticks Tick Borne Dis. 2018; 9(5):1364-1371. PMC: 6052676. DOI: 10.1016/j.ttbdis.2018.05.015. View

12.

Mehand M, Al-Shorbaji F, Millett P, Murgue B . The WHO R&D Blueprint: 2018 review of emerging infectious diseases requiring urgent research and development efforts. Antiviral Res. 2018; 159:63-67. PMC: 7113760. DOI: 10.1016/j.antiviral.2018.09.009. View

13.

Pecere T, Sarinella F, Salata C, Gatto B, Bet A, Dalla Vecchia F . Involvement of p53 in specific anti-neuroectodermal tumor activity of aloe-emodin. Int J Cancer. 2003; 106(6):836-47. DOI: 10.1002/ijc.11312. View

14.

Bente D, Forrester N, Watts D, McAuley A, Whitehouse C, Bray M . Crimean-Congo hemorrhagic fever: history, epidemiology, pathogenesis, clinical syndrome and genetic diversity. Antiviral Res. 2013; 100(1):159-89. DOI: 10.1016/j.antiviral.2013.07.006. View

15.

Browning R, Adamson S, Karim S . Choice of a stable set of reference genes for qRT-PCR analysis in Amblyomma maculatum (Acari: Ixodidae). J Med Entomol. 2012; 49(6):1339-46. DOI: 10.1603/me12123. View

16.

Haas B, Papanicolaou A, Yassour M, Grabherr M, Blood P, Bowden J . De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nat Protoc. 2013; 8(8):1494-512. PMC: 3875132. DOI: 10.1038/nprot.2013.084. View

17.

Nijhof A, Balk J, Postigo M, Jongejan F . Selection of reference genes for quantitative RT-PCR studies in Rhipicephalus (Boophilus) microplus and Rhipicephalus appendiculatus ticks and determination of the expression profile of Bm86. BMC Mol Biol. 2009; 10:112. PMC: 2809063. DOI: 10.1186/1471-2199-10-112. View