Study on Collagen Parameters in Vulvar Cancer and Preneoplastic Lesions by Second Harmonic Generation Microscopy

Overview

Journal Sci Rep

Specialty Science

Date 2020 Mar 30

PMID 32221321

Citations 5

Authors

Maria das Gracas de Fatima Cavalcanti Castor

Leuridan Cavalcante Torres

Roberto Jose Vieira DE Mello

Rodrigo de Andrade Natal

Jose Vassallo

Affiliations

Soon will be listed here.

Abstract

The extracellular matrix plays an important role in cellular balance, and collagen fibers are its most important component. Over the last few years, second harmonic generation (SHG) microscopy has been used for the analysis of collagen fibers in several types of gynaecological cancers, such as breast and ovarian cancer. The value of collagen parameters obtained with this technique to gain insights on the physiopathology and on the prognostic evaluation of cancer has been advocated. Herein, we have characterized the collagen fibers in squamous cell carcinoma (VSCC) and preneoplastic lesions using the SHG microscopy. Collagen parameters, quantity, organization, and uniformity, of VSCC, adjacent skin of VSCC or preneoplastic lesions were compared with values obtained in normal tissue of healthy control. There was an evident decrease in the values of collagen fiber parameters in the VSCC. Increased quantity and uniformity of tumor associated collagen fibers were associated with the presence of lymph node metastases, which suggest a prognostic value of such parameters in the evaluation of vulvar cancer.

Citing Articles

Biological implications of decoding the extracellular matrix of vulva cancer.

Islam M, Debnath K, Moniruzzaman R, Okuyama K, Islam S, Dongre H Oncol Rep. 2024; 53(2).

PMID: 39670289 PMC: 11652961. DOI: 10.3892/or.2024.8852.

Imaging at the nexus: how state of the art imaging techniques can enhance our understanding of cancer and fibrosis.

Baniasadi A, Das J, Prendergast C, Beizavi Z, Ma H, Jaber M J Transl Med. 2024; 22(1):567.

PMID: 38872212 PMC: 11177383. DOI: 10.1186/s12967-024-05379-1.

Collagen Remodeling along Cancer Progression Providing a Novel Opportunity for Cancer Diagnosis and Treatment.

Song K, Yu Z, Zu X, Li G, Hu Z, Xue Y Int J Mol Sci. 2022; 23(18).

PMID: 36142424 PMC: 9502421. DOI: 10.3390/ijms231810509.

Expression Profiles Reveal Involvement of , , , and in Vulvar Carcinogenesis.

Zhang T, Liu Q, Yu M, Lan Y, Zhou J Technol Cancer Res Treat. 2021; 20:15330338211004922.

PMID: 33888009 PMC: 8071978. DOI: 10.1177/15330338211004922.

Intratumoral collagen signatures predict clinical outcomes in feline mammary carcinoma.

Rosen S, Brisson B, Durham A, Munroe C, McNeill C, Stefanovski D PLoS One. 2020; 15(8):e0236516.

PMID: 32776970 PMC: 7416937. DOI: 10.1371/journal.pone.0236516.

References

Adur J, Pelegati V, Costa L, Pietro L, de Thomaz A, Almeida D . Recognition of serous ovarian tumors in human samples by multimodal nonlinear optical microscopy. J Biomed Opt. 2011; 16(9):096017. DOI: 10.1117/1.3626575. View

Burke K, Tang P, Brown E . Second harmonic generation reveals matrix alterations during breast tumor progression. J Biomed Opt. 2012; 18(3):31106. PMC: 3595714. DOI: 10.1117/1.JBO.18.3.031106. View

Raine-Fenning N, Brincat M, Muscat-Baron Y . Skin aging and menopause : implications for treatment. Am J Clin Dermatol. 2003; 4(6):371-8. DOI: 10.2165/00128071-200304060-00001. View

Rezakhaniha R, Agianniotis A, Schrauwen J, Griffa A, Sage D, Bouten C . Experimental investigation of collagen waviness and orientation in the arterial adventitia using confocal laser scanning microscopy. Biomech Model Mechanobiol. 2011; 11(3-4):461-73. DOI: 10.1007/s10237-011-0325-z. View

Drifka C, Loeffler A, Mathewson K, Mehta G, Keikhosravi A, Liu Y . Comparison of Picrosirius Red Staining With Second Harmonic Generation Imaging for the Quantification of Clinically Relevant Collagen Fiber Features in Histopathology Samples. J Histochem Cytochem. 2016; 64(9):519-29. PMC: 5006137. DOI: 10.1369/0022155416659249. View

van de Nieuwenhof H, Bulten J, Hollema H, Dommerholt R, Massuger L, van der Zee A . Differentiated vulvar intraepithelial neoplasia is often found in lesions, previously diagnosed as lichen sclerosus, which have progressed to vulvar squamous cell carcinoma. Mod Pathol. 2010; 24(2):297-305. DOI: 10.1038/modpathol.2010.192. View

van de Nieuwenhof H, van der Avoort I, de Hullu J . Review of squamous premalignant vulvar lesions. Crit Rev Oncol Hematol. 2008; 68(2):131-56. DOI: 10.1016/j.critrevonc.2008.02.012. View

Gasimli K, Straussner M, Schmeil I, Karn T, Winkelmann R, Becker S . Impact of re-excision of residual adjacent vulvar intraepithelial neoplasia (VIN III) and histological tumour-free margin (hTFM) on survival in primary squamous cell carcinoma of vulva. Arch Gynecol Obstet. 2018; 298(5):945-950. DOI: 10.1007/s00404-018-4887-1. View

Chen W, Chou C, Lin M, Chen Y, Jee S, Tan H . Single-wavelength reflected confocal and multiphoton microscopy for tissue imaging. J Biomed Opt. 2009; 14(5):054026. DOI: 10.1117/1.3247157. View

10.

Lanneau G, Argenta P, Lanneau M, Riffenburgh R, Gold M, McMeekin D . Vulvar cancer in young women: demographic features and outcome evaluation. Am J Obstet Gynecol. 2009; 200(6):645.e1-5. DOI: 10.1016/j.ajog.2009.01.014. View

11.

Ueda Y, Enomoto T, Kimura T, Yoshino K, Fujita M, Kimura T . Two distinct pathways to development of squamous cell carcinoma of the vulva. J Skin Cancer. 2010; 2011:951250. PMC: 3003991. DOI: 10.1155/2011/951250. View

12.

Wang Z, Trope C, Suo Z, Troen G, Yang G, Nesland J . The clinicopathological and prognostic impact of 14-3-3 sigma expression on vulvar squamous cell carcinomas. BMC Cancer. 2008; 8:308. PMC: 2577690. DOI: 10.1186/1471-2407-8-308. View

13.

Hanley C, Noble F, Ward M, Bullock M, Drifka C, Mellone M . A subset of myofibroblastic cancer-associated fibroblasts regulate collagen fiber elongation, which is prognostic in multiple cancers. Oncotarget. 2015; 7(5):6159-74. PMC: 4868747. DOI: 10.18632/oncotarget.6740. View

14.

Joura E, Losch A, Breitenecker G, Leodolter S . Trends in vulvar neoplasia. Increasing incidence of vulvar intraepithelial neoplasia and squamous cell carcinoma of the vulva in young women. J Reprod Med. 2000; 45(8):613-5. View

15.

Brooks M, Mo Q, Krasnow R, Ho P, Lee Y, Xiao J . Positive association of collagen type I with non-muscle invasive bladder cancer progression. Oncotarget. 2016; 7(50):82609-82619. PMC: 5347718. DOI: 10.18632/oncotarget.12089. View

16.

Weiskopf D, Weinberger B, Grubeck-Loebenstein B . The aging of the immune system. Transpl Int. 2009; 22(11):1041-50. DOI: 10.1111/j.1432-2277.2009.00927.x. View

17.

Sideri M, Jones R, Wilkinson E, Preti M, Heller D, Scurry J . Squamous vulvar intraepithelial neoplasia: 2004 modified terminology, ISSVD Vulvar Oncology Subcommittee. J Reprod Med. 2006; 50(11):807-10. View

18.

Saraiya M, Watson M, Wu X, King J, Chen V, Smith J . Incidence of in situ and invasive vulvar cancer in the US, 1998-2003. Cancer. 2008; 113(10 Suppl):2865-72. DOI: 10.1002/cncr.23759. View

19.

Calleja-Agius J, Brincat M, Borg M . Skin connective tissue and ageing. Best Pract Res Clin Obstet Gynaecol. 2013; 27(5):727-40. DOI: 10.1016/j.bpobgyn.2013.06.004. View

20.

Alkatout I, Schubert M, Garbrecht N, Weigel M, Jonat W, Mundhenke C . Vulvar cancer: epidemiology, clinical presentation, and management options. Int J Womens Health. 2015; 7:305-13. PMC: 4374790. DOI: 10.2147/IJWH.S68979. View