Generation of a Murine SWATH-MS Spectral Library to Quantify More Than 11,000 Proteins

Overview

Journal Sci Data

Specialty Science

Date 2020 Mar 29

PMID 32218446

Citations 9

Authors

Chuan-Qi Zhong

Jianfeng Wu

Xingfeng Qiu

Xi Chen

Changchuan Xie

Jiahuai Han

Affiliations

Soon will be listed here.

Abstract

Targeted SWATH-MS data analysis is critically dependent on the spectral library. Comprehensive spectral libraries of human or several other organisms have been published, but the extensive spectral library for mouse, a widely used model organism is not available. Here, we present a large murine spectral library covering more than 11,000 proteins and 240,000 proteotypic peptides, which included proteins derived from 9 murine tissue samples and one murine L929 cell line. This resource supports the quantification of 67% of all murine proteins annotated by UniProtKB/Swiss-Prot. Furthermore, we applied the spectral library to SWATH-MS data from murine tissue samples. Data are available via SWATHAtlas (PASS01441).

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