Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates

Overview

Journal Int J Pept Res Ther

Publisher Springer

Date 2020 Mar 28

PMID 32214925

Citations 1

Authors

Brian M Polster

Rafael Arze

Matthew H Lyttle

David G Nicholls

Derek Hudson

Affiliations

Soon will be listed here.

Abstract

A step-by-step evaluation of dual-labeled FRET substrates for the protease calpain is reported. The study led to cell permeable selections, with optimized specificity and effectiveness for the target enzyme, and improved stability to non-specific degrading enzymes.

Citing Articles

Calpain-Independent Intracellular Protease Activity Is Elevated in Excitotoxic Cortical Neurons Prior to Delayed Calcium Deregulation and Mitochondrial Dysfunction.

Polster B, Mark K, Arze R, Hudson D Biomolecules. 2022; 12(7).

PMID: 35883560 PMC: 9313431. DOI: 10.3390/biom12071004.

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