Longitudinal Mouse-PET Imaging: a Reliable Method for Estimating Binding Parameters Without a Reference Region or Blood Sampling

Overview

Journal Eur J Nucl Med Mol Imaging

Specialties Biophysics
Nuclear Medicine
Radiology

Date 2020 Mar 27

PMID 32211931

Citations 5

Authors

Catriona Wimberley

Duc Loc Nguyen

Charles Truillet

Marie-Anne Peyronneau

Zuhal Gulhan

Matteo Tonietto

Fawzi Boumezbeur

Raphael Boisgard

Sylvie Chalon

Viviane Bouilleret

Irene Buvat

Affiliations

Soon will be listed here.

Abstract

Methods: Using dynamic, whole-body F-DPA-714 PET scans (60 min) in a mouse model of hippocampal sclerosis, we applied a factor analysis (FA) approach to extract an image-derived input function (IDIF). This mouse-specific IDIF was then used for 4D-resolution recovery and denoising (4D-RRD) that outputs a dynamic image with better spatial resolution and noise properties, and a map of the total volume of distribution (V) was obtained using a basis function approach in a total of 9 mice with 4 longitudinal PET scans each. We also calculated percent injected dose (%ID) with and without 4D-RRD. The V and %ID parameters were compared to quantified ex vivo autoradiography using regional correlations of the specific binding from autoradiography against V and %ID parameters.

Results: The peaks of the IDIFs were strongly correlated with the injected dose (Pearson R = 0.79). The regional correlations between the %ID estimates and autoradiography were R = 0.53 without 4D-RRD and 0.72 with 4D-RRD over all mice and scans. The regional correlations between the V estimates and autoradiography were R = 0.66 without 4D-RRD and 0.79 with application of 4D-RRD over all mice and scans.

Conclusion: We present a FA approach for IDIF extraction which is robust, reproducible and can be used in quantification methods for resolution recovery, denoising and parameter estimation. We demonstrated that the proposed quantification method yields parameter estimates closer to ex vivo measurements than semi-quantitative methods such as %ID and is immune to tracer binding in tissue unlike reference tissue methods. This approach allows for accurate quantification in longitudinal PET studies in mice while avoiding repeated blood sampling.

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