High-protein Diet and Omega-3 Fatty Acids Improve Redox Status in Olanzapine-treated Rats

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2020 Mar 25

PMID 32206947

Citations 1

Authors

Miroslav Mitrovic

Tamara Nikolic Turnic

Vladimir Zivkovic

Zarko Pavic

Aleksandra Vranic

Ivan Srejovic

Jasmina Sretenovic

Sergey Bolevich

Vladimir Lj Jakovljevic

Affiliations

Soon will be listed here.

Abstract

The present study aimed to estimate the effects of high-protein diet (PD)-isolated whey protein and omega-3 fatty acids-docosahexaenoic and eicosapentaenoic acid on oxidative parameters of rats treated with Olanzapine (OLZ). Experiments were carried out on 8-week-old Wistar albino male rats (n = 64) weighing 200 ± 20 g. By dietary and pharmacological treatment, all animals were divided into 8 groups: 1. CTRL group; 2. CTRL + OLZ group; 3. CTRL + FA group; 4. CTRL + OLZ + FA group; 5. PD group; 6. PD + OLZ group; 7. PD + FA group; 8. PD + OLZ + FA group. After 6 weeks of pharmacological/diet treatment, all animals were sacrificed to collect blood samples and determine the biomarkers of oxidative stress. The following oxidative stress markers were measured spectrophotometrically: superoxide anion radical (O), hydrogen peroxide (HO), nitric oxide (NO), index of lipid peroxidation measured as TBARS, reduced glutathione, catalase and superoxide dismutase. The study has shown that Olanzapine treatment was associated with increased release of pro-oxidants and diminished activity of anti-oxidant markers. Additional supplementation with PD and FA succeeded in abolishing the negative influence in most of the measured parameters. However, these beneficial impacts were stronger in the case of their separate application, which could be the practical and clinical importance of these results.

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