Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells

Overview

Journal J Clin Med

Specialty General Medicine

Date 2020 Mar 22

PMID 32197458

Citations 15

Authors

Kyeongseok Kim

Minchan Gil

Ahmed Abdal Dayem

Sangbaek Choi

Geun-Ho Kang

Gwang-Mo Yang

Sungha Cho

Yeojin Jeong

Se Jong Kim

Jaekwon Seok

Hee Jeong Kwak

Subbroto Kumar Saha

Aram Kim

Ssang-Goo Cho

Affiliations

Soon will be listed here.

Abstract

The availability of autologous adult stem cells is one of the essential prerequisites for human stem cell therapy. Urine-derived stem cells (USCs) are considered as desirable cell sources for cell therapy because donor-specific USCs are easily and non-invasively obtained from urine. Efficient isolation, expansion, and differentiation methods of USCs are necessary to increase their availability. Here, we developed a method for efficient isolation and expansion of USCs using Matrigel, and the rho-associated protein kinase (ROCK) inhibitor, Y-27632. The prepared USCs showed significantly enhanced migration, colony forming capacity, and differentiation into osteogenic or chondrogenic lineage. The USCs were successfully reprogramed into induced pluripotent stem cells (USC-iPSCs) and further differentiated into kidney organoid and hematopoietic progenitor cells (HPCs). Using flavonoid molecules, the isolation efficiency of USCs and the production of HPCs from the USC-iPSCs was increased. Taken together, we present an improved isolation method of USCs utilizing Matrigel, a ROCK inhibitor and flavonoids, and enhanced differentiation of USC-iPSC to HPC by flavonoids. These novel findings could significantly enhance the use of USCs and USC-iPSCs for stem cell research and further application in regenerative stem cell-based therapies.

Citing Articles

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Yin X, Li Q, Shu Y, Wang H, Thomas B, Maxwell J J Biomed Sci. 2024; 31(1):47.

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Urine-derived stem cells: Promising advancements and applications in regenerative medicine and beyond.

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