Phycobiliproteins from (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity

Overview

Journal Nutrients

Date 2020 Mar 22

PMID 32197331

Citations 29

Authors

Yuchen Li

Gilda Aiello

Carlotta Bollati

Martina Bartolomei

Anna Arnoldi

Carmen Lammi

Affiliations

Soon will be listed here.

Abstract

(spirulina) is a cyanobacterium, which contains mainly two phycobiliproteins (PBP), i.e., C-phycocyanin (C-PC) and allophycocyanin (APC). In this study, PBP were hydrolyzed using trypsin, and the composition of the hydrolysate was characterized by HPLC-ESI-MS/MS. Furthermore, the potential anti-diabetic activity was assessed by using either biochemical or cellular techniques. Findings suggest that PBP peptides inhibit DPP-IV activity in vitro with a dose-response trend and an IC value falling in the range between 0.5 and 1.0 mg/mL. A lower inhibition of the DPP-IV activity expressed by Caco-2 cells was observed, which was explained by a secondary metabolic degradation exerted by the same cells.

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References

Lammi C, Zanoni C, Scigliuolo G, DAmato A, Arnoldi A . Lupin peptides lower low-density lipoprotein (LDL) cholesterol through an up-regulation of the LDL receptor/sterol regulatory element binding protein 2 (SREBP2) pathway at HepG2 cell line. J Agric Food Chem. 2014; 62(29):7151-9. DOI: 10.1021/jf500795b. View

Ou Y, Ren Z, Wang J, Yang X . Phycocyanin ameliorates alloxan-induced diabetes mellitus in mice: Involved in insulin signaling pathway and GK expression. Chem Biol Interact. 2016; 247:49-54. DOI: 10.1016/j.cbi.2016.01.018. View

Lammi C, Arnoldi A, Aiello G . Soybean Peptides Exert Multifunctional Bioactivity Modulating 3-Hydroxy-3-Methylglutaryl-CoA Reductase and Dipeptidyl Peptidase-IV Targets in Vitro. J Agric Food Chem. 2019; 67(17):4824-4830. DOI: 10.1021/acs.jafc.9b01199. View

Natoli M, Leoni B, DAgnano I, DOnofrio M, Brandi R, Arisi I . Cell growing density affects the structural and functional properties of Caco-2 differentiated monolayer. J Cell Physiol. 2010; 226(6):1531-43. DOI: 10.1002/jcp.22487. View

Howell S, Brewis I, Hooper N, Kenny A, Turner A . Mosaic expression of membrane peptidases by confluent cultures of Caco-2 cells. FEBS Lett. 1993; 317(1-2):109-12. DOI: 10.1016/0014-5793(93)81502-q. View

Ou Y, Lin L, Pan Q, Yang X, Cheng X . Preventive effect of phycocyanin from Spirulina platensis on alloxan-injured mice. Environ Toxicol Pharmacol. 2012; 34(3):721-6. DOI: 10.1016/j.etap.2012.09.016. View

Rimbau V, Camins A, Pubill D, Sureda F, Romay C, Gonzalez R . C-phycocyanin protects cerebellar granule cells from low potassium/serum deprivation-induced apoptosis. Naunyn Schmiedebergs Arch Pharmacol. 2001; 364(2):96-104. DOI: 10.1007/s002100100437. View

Cherng S, Cheng S, Tarn A, Chou T . Anti-inflammatory activity of c-phycocyanin in lipopolysaccharide-stimulated RAW 264.7 macrophages. Life Sci. 2007; 81(19-20):1431-5. DOI: 10.1016/j.lfs.2007.09.009. View

Gao Y, Liao G, Xiang C, Yang X, Cheng X, Ou Y . Effects of phycocyanin on INS-1 pancreatic β-cell mediated by PI3K/Akt/FoxO1 signaling pathway. Int J Biol Macromol. 2015; 83:185-94. DOI: 10.1016/j.ijbiomac.2015.11.054. View

10.

Lammi C, Bollati C, Gelain F, Arnoldi A, Pugliese R . Enhancement of the Stability and Anti-DPPIV Activity of Hempseed Hydrolysates Through Self-Assembling Peptide-Based Hydrogels. Front Chem. 2019; 6:670. PMC: 6353790. DOI: 10.3389/fchem.2018.00670. View

11.

Furuta T, Miyabe Y, Yasui H, Kinoshita Y, Kishimura H . Angiotensin I Converting Enzyme Inhibitory Peptides Derived from Phycobiliproteins of Dulse Palmaria palmata. Mar Drugs. 2016; 14(2). PMC: 4771985. DOI: 10.3390/md14020032. View

12.

Power O, Nongonierma A, Jakeman P, FitzGerald R . Food protein hydrolysates as a source of dipeptidyl peptidase IV inhibitory peptides for the management of type 2 diabetes. Proc Nutr Soc. 2013; 73(1):34-46. DOI: 10.1017/S0029665113003601. View

13.

Ou Y, Lin L, Yang X, Pan Q, Cheng X . Antidiabetic potential of phycocyanin: effects on KKAy mice. Pharm Biol. 2013; 51(5):539-44. DOI: 10.3109/13880209.2012.747545. View

14.

Aiello G, Lammi C, Boschin G, Zanoni C, Arnoldi A . Exploration of Potentially Bioactive Peptides Generated from the Enzymatic Hydrolysis of Hempseed Proteins. J Agric Food Chem. 2017; 65(47):10174-10184. DOI: 10.1021/acs.jafc.7b03590. View

15.

Yoshida A, Takagaki Y, Nishimune T . Enzyme immunoassay for phycocyanin as the main component of spirulina color in foods. Biosci Biotechnol Biochem. 1996; 60(1):57-60. DOI: 10.1271/bbb.60.57. View

16.

Nongonierma A, FitzGerald R . Structure activity relationship modelling of milk protein-derived peptides with dipeptidyl peptidase IV (DPP-IV) inhibitory activity. Peptides. 2016; 79:1-7. DOI: 10.1016/j.peptides.2016.03.005. View

17.

Bhat V, Madyastha K . Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from Spirulina platensis: protection against oxidative damage to DNA. Biochem Biophys Res Commun. 2001; 285(2):262-6. DOI: 10.1006/bbrc.2001.5195. View

18.

Lammi C, Bollati C, Ferruzza S, Ranaldi G, Sambuy Y, Arnoldi A . Soybean- and Lupin-Derived Peptides Inhibit DPP-IV Activity on In Situ Human Intestinal Caco-2 Cells and Ex Vivo Human Serum. Nutrients. 2018; 10(8). PMC: 6115767. DOI: 10.3390/nu10081082. View

19.

Nauck M, Baller B, Meier J . Gastric inhibitory polypeptide and glucagon-like peptide-1 in the pathogenesis of type 2 diabetes. Diabetes. 2004; 53 Suppl 3:S190-6. DOI: 10.2337/diabetes.53.suppl_3.s190. View

20.

Pinero Estrada J, Bermejo Bescos P, Villar del Fresno A . Antioxidant activity of different fractions of Spirulina platensis protean extract. Farmaco. 2001; 56(5-7):497-500. DOI: 10.1016/s0014-827x(01)01084-9. View