Morin Attenuates High-fat Diet Induced-obesity Related Vascular Endothelial Dysfunction in Wistar Albino Rats

Overview

Journal Saudi Pharm J

Specialty Pharmacy

Date 2020 Mar 21

PMID 32194331

Citations 4

Authors

Hassan A Madkhali

Affiliations

Soon will be listed here.

Abstract

Vascular endothelial dysfunction is caused by dyslipidemia, hypertension, and deficiency of antioxidant systems. In this study, the protective effect of a flavonol, morin was investigated in high-fat diet (HFD)-induced dyslipidemia and vascular endothelium dysfunction. The dose-dependent attenuating effect of morin was tested at doses of 50 and 100 mg/kg/day in an model of HFD-induced dyslipidemia using rats whereas vascular endothelial reactivity was assessed in isolated rat aorta using organ bath setup. Morin administration in HFD-induced dyslipidemic rats for three weeks, resulted in a significant decrease in the body weight, LW/BW ratio as compared to rats treated with HFD only where the increase in body weight was observed. Significant reduction in the waist, BMI and lee index was also observed after morin treatment in HFD-induced dyslipidemic rats. In the lipid profile studies, HFD group showed a significant increase in the total cholesterol, triglyceride, LDL, and VLDL levels while HDL levels were decreased significantly, whereas morin treatment reversed all these parameters which were comparable to standard diet (SD) group. In the isolated aorta studies, HFD-induced endothelium dysfunction was observed, whereas it was reversed in the aorta of animals treated with morin at doses of 50 and 100 mg/kg/day, comparable to SD group. Morin treatment produced dose-dependent improvement in lipid profile and vascular endothelium protection, thus rationalizing its medicinal use in dyslipidemia and cardiovascular-related endothelial disorders.

Citing Articles

Morin, as a natural flavonoid, provides promising influences against cardiovascular diseases.

Khademi R, Mirzaei A, Mirzaei A, Edjlali F, Askari V, Baradaran Rahimi V Naunyn Schmiedebergs Arch Pharmacol. 2025; .

PMID: 39808314 DOI: 10.1007/s00210-024-03783-4.

Morin Ameliorates Myocardial Injury in Diabetic Rats via Modulation of Autophagy, Apoptosis, Inflammation, and Oxidative Stress.

Zhu L, He J Diabetes Metab Syndr Obes. 2025; 17:4867-4882.

PMID: 39742288 PMC: 11687097. DOI: 10.2147/DMSO.S476867.

Morin Prevents Non-Alcoholic Hepatic Steatosis in Obese Rats by Targeting the Peroxisome Proliferator-Activated Receptor Alpha (PPARα).

Al-Harbi L Life (Basel). 2024; 14(8).

PMID: 39202687 PMC: 11355712. DOI: 10.3390/life14080945.

Mitigation effect of galangin against aortic dysfunction and hypertrophy in rats with metabolic syndrome.

Pakdeechote P, Poasakate A, Prasatthong P, Potue P, Khamseekaew J, Maneesai P Heliyon. 2023; 9(5):e16500.

PMID: 37251824 PMC: 10213349. DOI: 10.1016/j.heliyon.2023.e16500.

Time-Restricted Feeding Improved Vascular Endothelial Function in a High-Fat Diet-Induced Obesity Rat Model.

Azemi A, Siti-Sarah A, Mokhtar S, Rasool A Vet Sci. 2022; 9(5).

PMID: 35622745 PMC: 9147025. DOI: 10.3390/vetsci9050217.

References

Buettner R, Scholmerich J, Bollheimer L . High-fat diets: modeling the metabolic disorders of human obesity in rodents. Obesity (Silver Spring). 2007; 15(4):798-808. DOI: 10.1038/oby.2007.608. View

Ahmad B, Rehman M, Amin I, Mir M, Ahmad S, Farooq A . Zingerone (4-(4-hydroxy-3-methylphenyl) butan-2-one) protects against alloxan-induced diabetes via alleviation of oxidative stress and inflammation: Probable role of NF-kB activation. Saudi Pharm J. 2018; 26(8):1137-1145. PMC: 6260481. DOI: 10.1016/j.jsps.2018.07.001. View

Costa R, Villela N, Souza M, Boa B, Cyrino F, Silva S . High fat diet induces central obesity, insulin resistance and microvascular dysfunction in hamsters. Microvasc Res. 2011; 82(3):416-22. DOI: 10.1016/j.mvr.2011.08.007. View

Daniel R, Devi K, Augusti K, Nair C . Mechanism of action of antiatherogenic and related effects of Ficus bengalensis Linn. flavonoids in experimental animals. Indian J Exp Biol. 2004; 41(4):296-303. View

Vanhoutte P, Boulanger C . Endothelium-dependent responses in hypertension. Hypertens Res. 1995; 18(2):87-98. DOI: 10.1291/hypres.18.87. View

Bigornia S, Mott M, Hess D, Apovian C, McDonnell M, Duess M . Long-term successful weight loss improves vascular endothelial function in severely obese individuals. Obesity (Silver Spring). 2010; 18(4):754-9. PMC: 2857970. DOI: 10.1038/oby.2009.482. View

Seiler C, Hess O, Buechi M, Suter T, KRAYENBUEHL H . Influence of serum cholesterol and other coronary risk factors on vasomotion of angiographically normal coronary arteries. Circulation. 1993; 88(5 Pt 1):2139-48. DOI: 10.1161/01.cir.88.5.2139. View

Luscher T . Imbalance of endothelium-derived relaxing and contracting factors. A new concept in hypertension?. Am J Hypertens. 1990; 3(4):317-30. DOI: 10.1093/ajh/3.4.317. View

Al-Nozha M, Al-Mazrou Y, Al-Maatouq M, Arafah M, Khalil M, Khan N . Obesity in Saudi Arabia. Saudi Med J. 2005; 26(5):824-9. View

10.

Jakobsdottir G, Xu J, Molin G, Ahrne S, Nyman M . High-fat diet reduces the formation of butyrate, but increases succinate, inflammation, liver fat and cholesterol in rats, while dietary fibre counteracts these effects. PLoS One. 2013; 8(11):e80476. PMC: 3827442. DOI: 10.1371/journal.pone.0080476. View

11.

Sergediene E, Jonsson K, Szymusiak H, Tyrakowska B, Rietjens I, Cenas N . Prooxidant toxicity of polyphenolic antioxidants to HL-60 cells: description of quantitative structure-activity relationships. FEBS Lett. 2000; 462(3):392-6. DOI: 10.1016/s0014-5793(99)01561-6. View

12.

Kobayasi R, Akamine E, Davel A, Rodrigues M, Carvalho C, Rossoni L . Oxidative stress and inflammatory mediators contribute to endothelial dysfunction in high-fat diet-induced obesity in mice. J Hypertens. 2010; 28(10):2111-9. DOI: 10.1097/HJH.0b013e32833ca68c. View

13.

Berg A, Scherer P . Adipose tissue, inflammation, and cardiovascular disease. Circ Res. 2005; 96(9):939-49. DOI: 10.1161/01.RES.0000163635.62927.34. View

14.

Vane J, Anggard E, Botting R . Regulatory functions of the vascular endothelium. N Engl J Med. 1990; 323(1):27-36. DOI: 10.1056/NEJM199007053230106. View

15.

Kapoor R, Kakkar P . Protective role of morin, a flavonoid, against high glucose induced oxidative stress mediated apoptosis in primary rat hepatocytes. PLoS One. 2012; 7(8):e41663. PMC: 3416810. DOI: 10.1371/journal.pone.0041663. View

16.

Steinberg H, Chaker H, LEAMING R, Johnson A, Brechtel G, Baron A . Obesity/insulin resistance is associated with endothelial dysfunction. Implications for the syndrome of insulin resistance. J Clin Invest. 1996; 97(11):2601-10. PMC: 507347. DOI: 10.1172/JCI118709. View

17.

Vijayakumar R, Surya D, Nalini N . Antioxidant efficacy of black pepper (Piper nigrum L.) and piperine in rats with high fat diet induced oxidative stress. Redox Rep. 2004; 9(2):105-10. DOI: 10.1179/135100004225004742. View

18.

Paoli P, Cirri P, Caselli A, Ranaldi F, Bruschi G, Santi A . The insulin-mimetic effect of Morin: a promising molecule in diabetes treatment. Biochim Biophys Acta. 2013; 1830(4):3102-11. DOI: 10.1016/j.bbagen.2013.01.017. View

19.

Tian W . Inhibition of fatty acid synthase by polyphenols. Curr Med Chem. 2006; 13(8):967-77. DOI: 10.2174/092986706776361012. View

20.

Liang Z, Liu F, Grundke-Iqbal I, Iqbal K, Gong C . Down-regulation of cAMP-dependent protein kinase by over-activated calpain in Alzheimer disease brain. J Neurochem. 2007; 103(6):2462-70. PMC: 2262109. DOI: 10.1111/j.1471-4159.2007.04942.x. View