Chemical-defined Medium Supporting the Expansion of Human Mesenchymal Stem Cells

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2020 Mar 21

PMID 32192530

Citations 12

Authors

Jianyong Xu

Wei Lian

Jieting Chen

Wenlei Li

Lingyun Li

Zhong Huang

Affiliations

Soon will be listed here.

Abstract

Objectives: Mesenchymal stem cells (MSCs) have been intensively investigated as to their therapeutic potentials. However, the full chemical-defined medium supporting the isolation and expansion of human MSCs has not been developed yet.

Materials And Methods: Here, we developed the full chemical-defined medium, NBVbe medium, via RNA sequencing, bioinformatic analysis, and growth factor screening.

Results: The NBVbe medium contains N2B27 medium with the BSA (bovine serum albumin) replaced by the recombinant human albumin, bFGF (basic fibroblast growth factor), vitamin C, and EGF (epidermal growth factor). The NBVbe medium could support the isolation and expansion of human MSCs from the umbilical cords.

Conclusions: The full chemical-defined medium supporting the isolation and expansion of human MSCs has been developed. This would be helpful for further optimization of the MSC medium, their clinical applications, and molecular characterization.

Citing Articles

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