A Label-Free Immunosensor Based on Graphene Oxide/FeO/Prussian Blue Nanocomposites for the Electrochemical Determination of HBsAg

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2020 Mar 19

PMID 32183297

Citations 10

Authors

Shanshan Wei

Haolin Xiao

LiangLi Cao

Zhencheng Chen

Affiliations

Soon will be listed here.

Abstract

In this article, a highly sensitive label-free immunosensor based on a graphene oxide (GO)/FeO/Prussian blue (PB) nanocomposite modified electrode was developed for the determination of human hepatitis B surface antigen (HBsAg). In this electrochemical immunoassay system, PB was used as a redox probe, while GO/FeO/PB nanocomposites and AuNPs were prepared and coated on screen-printed electrodes to enhance the detection sensitivity and to immobilize the hepatitis B surface antibody (HBsAb). The immunosensor was fabricated based on the principle that the decrease in peak currents of PB is proportional to the concentration of HBsAg captured on the modified immunosensor. The experimental results revealed that the immunosensor exhibited a sensitive response to HBsAg in the range of 0.5 pg·mL to 200 ng·mL, and with a low detection limit of 0.166 pg·mL (S/N = 3). Furthermore, the proposed immunosensor was used to detect several clinical serum samples with acceptable results, and it also showed good reproducibility, selectivity and stability, which may have a promising potential application in clinical immunoassays.

Citing Articles

Nanostructured Metal Oxide-Based Electrochemical Biosensors in Medical Diagnosis.

Keles G, Sifa Ataman E, Taskin S, Polatoglu I, Kurbanoglu S Biosensors (Basel). 2024; 14(5).

PMID: 38785712 PMC: 11117604. DOI: 10.3390/bios14050238.

The Advancement of Nanomaterials for the Detection of Hepatitis B Virus and Hepatitis C Virus.

Shi W, Li K, Zhang Y Molecules. 2023; 28(20).

PMID: 37894681 PMC: 10608909. DOI: 10.3390/molecules28207201.

Advanced Theranostic Strategies for Viral Hepatitis Using Carbon Nanostructures.

Gholami A, Mousavi S, Masoumzadeh R, Binazadeh M, Bagheri Lankarani K, Omidifar N Micromachines (Basel). 2023; 14(6).

PMID: 37374770 PMC: 10301519. DOI: 10.3390/mi14061185.

Electrochemical Label-free Methods for Ultrasensitive Multiplex Protein Profiling of Infectious Diseases.

Madhurantakam S, Churcher N, Kumar R, Prasad S Curr Med Chem. 2023; 31(25):3857-3869.

PMID: 37303174 DOI: 10.2174/0929867330666230609112052.

Functional Nanomaterials Enhancing Electrochemical Biosensors as Smart Tools for Detecting Infectious Viral Diseases.

Curulli A Molecules. 2023; 28(9).

PMID: 37175186 PMC: 10180161. DOI: 10.3390/molecules28093777.

References

Felix F, Angnes L . Electrochemical immunosensors - A powerful tool for analytical applications. Biosens Bioelectron. 2017; 102:470-478. DOI: 10.1016/j.bios.2017.11.029. View

Sun D, Li H, Li M, Li C, Qian L, Yang B . Electrochemical immunosensors with AuPt-vertical graphene/glassy carbon electrode for alpha-fetoprotein detection based on label-free and sandwich-type strategies. Biosens Bioelectron. 2019; 132:68-75. DOI: 10.1016/j.bios.2019.02.045. View

Ekins R . Ligand assays: from electrophoresis to miniaturized microarrays. Clin Chem. 1998; 44(9):2015-30. View

Makaraviciute A, Ramanaviciene A . Site-directed antibody immobilization techniques for immunosensors. Biosens Bioelectron. 2013; 50:460-71. DOI: 10.1016/j.bios.2013.06.060. View

Song Y, Luo Y, Zhu C, Li H, Du D, Lin Y . Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials. Biosens Bioelectron. 2015; 76:195-212. DOI: 10.1016/j.bios.2015.07.002. View

Han Y, Li P, Xu Y, Li H, Song Z, Nie Z . Fluorescent nanosensor for probing histone acetyltransferase activity based on acetylation protection and magnetic graphitic nanocapsules. Small. 2014; 11(7):877-85. DOI: 10.1002/smll.201401989. View

Stoller M, Park S, Zhu Y, An J, Ruoff R . Graphene-based ultracapacitors. Nano Lett. 2008; 8(10):3498-502. DOI: 10.1021/nl802558y. View

Nourani S, Ghourchian H, Boutorabi S . Magnetic nanoparticle-based immunosensor for electrochemical detection of hepatitis B surface antigen. Anal Biochem. 2013; 441(1):1-7. DOI: 10.1016/j.ab.2013.06.011. View

Ganem D, Prince A . Hepatitis B virus infection--natural history and clinical consequences. N Engl J Med. 2004; 350(11):1118-29. DOI: 10.1056/NEJMra031087. View

10.

Kim K, Zhao Y, Jang H, Lee S, Kim J, Kim K . Large-scale pattern growth of graphene films for stretchable transparent electrodes. Nature. 2009; 457(7230):706-10. DOI: 10.1038/nature07719. View

11.

Li F, Yu Y, Cui H, Yang D, Bian Z . Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platform. Analyst. 2013; 138(6):1844-50. DOI: 10.1039/c3an36805j. View

12.

Xi Z, Huang R, Li Z, He N, Wang T, Su E . Selection of HBsAg-Specific DNA Aptamers Based on Carboxylated Magnetic Nanoparticles and Their Application in the Rapid and Simple Detection of Hepatitis B Virus Infection. ACS Appl Mater Interfaces. 2015; 7(21):11215-23. DOI: 10.1021/acsami.5b01180. View

13.

Li H, He J, Li S, Turner A . Electrochemical immunosensor with N-doped graphene-modified electrode for label-free detection of the breast cancer biomarker CA 15-3. Biosens Bioelectron. 2013; 43:25-9. DOI: 10.1016/j.bios.2012.11.037. View

14.

Valaydon Z, Locarnini S . The virological aspects of hepatitis B. Best Pract Res Clin Gastroenterol. 2017; 31(3):257-264. DOI: 10.1016/j.bpg.2017.04.013. View

15.

Martinot-Peignoux M, Carvalho-Filho R, Lapalus M, Netto-Cardoso A, Lada O, Batrla R . Hepatitis B surface antigen serum level is associated with fibrosis severity in treatment-naïve, e antigen-positive patients. J Hepatol. 2013; 58(6):1089-95. DOI: 10.1016/j.jhep.2013.01.028. View

16.

Omidfar K, Khorsand F, Azizi M . New analytical applications of gold nanoparticles as label in antibody based sensors. Biosens Bioelectron. 2013; 43:336-47. DOI: 10.1016/j.bios.2012.12.045. View

17.

Feng T, Qiao X, Wang H, Sun Z, Hong C . A sandwich-type electrochemical immunosensor for carcinoembryonic antigen based on signal amplification strategy of optimized ferrocene functionalized Fe₃O₄@SiO₂ as labels. Biosens Bioelectron. 2015; 79:48-54. DOI: 10.1016/j.bios.2015.11.001. View

18.

Soeung S, Rani M, Huong V, Sarath S, Kimly C, Kohei T . Results from nationwide hepatitis B serosurvey in Cambodia using simple and rapid laboratory test: implications for National Immunization Program. Am J Trop Med Hyg. 2009; 81(2):252-7. View

19.

Kim J, Oh S, Shukla S, Hong S, Heo N, Bajpai V . Heteroassembled gold nanoparticles with sandwich-immunoassay LSPR chip format for rapid and sensitive detection of hepatitis B virus surface antigen (HBsAg). Biosens Bioelectron. 2018; 107:118-122. DOI: 10.1016/j.bios.2018.02.019. View

20.

Huang K, Niu D, Xie W, Wang W . A disposable electrochemical immunosensor for carcinoembryonic antigen based on nano-Au/multi-walled carbon nanotubes-chitosans nanocomposite film modified glassy carbon electrode. Anal Chim Acta. 2010; 659(1-2):102-8. DOI: 10.1016/j.aca.2009.11.023. View