» Articles » PMID: 32183164

Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells

Overview
Journal Cells
Publisher MDPI
Date 2020 Mar 19
PMID 32183164
Citations 12
Authors
Affiliations
Soon will be listed here.
Abstract

Human pluripotent stem cells (hPSCs) can provide unlimited supply for mesenchymal stem cells (MSCs) and adipocytes that can be used for therapeutic applications. Here we developed a simple and highly efficient all--retinoic acid (RA)-based method for generating an off-the-shelf and scalable number of human pluripotent stem cell (hPSC)-derived MSCs with enhanced adipogenic potential. We showed that short exposure of multiple hPSC lines (hESCs/hiPSCs) to 10 μM RA dramatically enhances embryoid body (EB) formation through regulation of genes activating signaling pathways associated with cell proliferation, survival and adhesion, among others. Disruption of cell adhesion induced the subsequent differentiation of the highly expanded RA-derived EB-forming cells into a pure population of multipotent MSCs (up to 1542-fold increase in comparison to RA-untreated counterparts). Interestingly, the RA-derived MSCs displayed enhanced differentiation potential into adipocytes. Thus, these findings present a novel RA-based approach for providing an unlimited source of MSCs and adipocytes that can be used for regenerative medicine, drug screening and disease modeling applications.

Citing Articles

When do the pathological signs become evident? Study of human mesenchymal stem cells in MDPL syndrome.

Paola S, Lara G, Michela M, Silvia D, Serena M, Rosalba P Aging (Albany NY). 2024; 16(22):13505-13525.

PMID: 39611849 PMC: 11723661. DOI: 10.18632/aging.206159.


Effect of hypoxia on proliferation and differentiation of induced pluripotent stem cell-derived mesenchymal stem cells.

Alwohoush E, Ismail M, Al-Kurdi B, Barham R, Al Hadidi S, Awidi A Heliyon. 2024; 10(19):e38857.

PMID: 39421364 PMC: 11483329. DOI: 10.1016/j.heliyon.2024.e38857.


Advancements and Innovative Strategies in Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cell Therapy: A Comprehensive Review.

Shi X, Zhang K, Yu F, Qi Q, Cai X, Zhang Y Stem Cells Int. 2024; 2024:4073485.

PMID: 39377039 PMC: 11458320. DOI: 10.1155/2024/4073485.


Emerging Landscape of Mesenchymal Stem Cell Senescence Mechanisms and Implications on Therapeutic Strategies.

Wang J, Zhang M, Wang H ACS Pharmacol Transl Sci. 2024; 7(8):2306-2325.

PMID: 39144566 PMC: 11320744. DOI: 10.1021/acsptsci.4c00284.


Maternal inappropriate calcium intake aggravates dietary-induced obesity in male offspring by affecting the differentiation potential of mesenchymal stem cells.

Li P, Wang Y, Li P, Liu Y, Liu W, Chen X World J Stem Cells. 2022; 14(10):756-776.

PMID: 36337156 PMC: 9630989. DOI: 10.4252/wjsc.v14.i10.756.


References
1.
van Harmelen V, Astrom G, Stromberg A, Sjolin E, Dicker A, Hovatta O . Differential lipolytic regulation in human embryonic stem cell-derived adipocytes. Obesity (Silver Spring). 2007; 15(4):846-52. DOI: 10.1038/oby.2007.595. View

2.
McBeath R, Pirone D, Nelson C, Bhadriraju K, Chen C . Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell. 2004; 6(4):483-95. DOI: 10.1016/s1534-5807(04)00075-9. View

3.
Brown P, Squire M, Li W . Characterization and evaluation of mesenchymal stem cells derived from human embryonic stem cells and bone marrow. Cell Tissue Res. 2014; 358(1):149-64. PMC: 4329984. DOI: 10.1007/s00441-014-1926-5. View

4.
Maden M . Retinoic acid in the development, regeneration and maintenance of the nervous system. Nat Rev Neurosci. 2007; 8(10):755-65. DOI: 10.1038/nrn2212. View

5.
Arpornmaeklong P, Brown S, Wang Z, Krebsbach P . Phenotypic characterization, osteoblastic differentiation, and bone regeneration capacity of human embryonic stem cell-derived mesenchymal stem cells. Stem Cells Dev. 2009; 18(7):955-68. PMC: 3032563. DOI: 10.1089/scd.2008.0310. View