The Effects of Fibrin-icariin Nanoparticle Loaded in Poly (lactic-co-glycolic) Acid Scaffold As a Localized Delivery System on Chondrogenesis of Human Adipose-derived Stem Cells

Overview

Journal Adv Biomed Res

Specialty Biomedical Engineering

Date 2020 Mar 18

PMID 32181230

Citations 3

Authors

Mona Gorji

Nazem Ghasemi

Mohsen Setayeshmehr

Anooshe Zargar

Mohammad Kazemi

Mitra Soleimani

Batool Hashemibeni

Affiliations

Soon will be listed here.

Abstract

Background: Nowadays, cartilage tissue engineering is the best candidate for regeneration of cartilage defects. This study evaluates the effect of fibrin/icariin (ICA) nanoparticles (F/I NPs) on chondrogenesis of stem cells.

Materials And Methods: F/I NPs were characterized by Dynamic Light Scattering DLS. Poly (lactic-co-glycolic) acid (PLGA)-F/I NP scaffold was fabricated and assessed by scanning electron microscope. Human adipose-derived stem cells (hADSCs) were seeded on scaffold and induced for chondrogenesis. After 14 days, cell viability and gene expression were analyzed by the 3-(4, 5- dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. MTT assay and real-time polymerase chain reaction (RT-PCR).

Results: The size and surface charge of F/I NP were about 28-30 nm and - 17, respectively. The average of pore size of PLGA and PLGA-fibrin/ICA was 230 and 340 μm, respectively. Cell viability of differentiated cells in P/F group was higher than others significantly ( ≤ 0.05). Furthermore, quantitative RT-PCR analysis demonstrated that ICA upregulated cartilaginous-specific gene expression. Furthermore, the results of the expression of type I collagen revealed that ICA downregulated this gene significantly ( < 0.01).

Conclusions: The results indicated that F/I NP could be a potential factor for chondrogenesis of stem cells and downregulation of fibrocartilage marker.

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References

Galvin P, Thompson D, Ryan K, McCarthy A, Moore A, Burke C . Nanoparticle-based drug delivery: case studies for cancer and cardiovascular applications. Cell Mol Life Sci. 2011; 69(3):389-404. PMC: 11115117. DOI: 10.1007/s00018-011-0856-6. View

Tautzenberger A, Kovtun A, Ignatius A . Nanoparticles and their potential for application in bone. Int J Nanomedicine. 2012; 7:4545-57. PMC: 3423651. DOI: 10.2147/IJN.S34127. View

Nakamura K, Shirai T, Morishita S, Uchida S, Makishima F . p38 mitogen-activated protein kinase functionally contributes to chondrogenesis induced by growth/differentiation factor-5 in ATDC5 cells. Exp Cell Res. 1999; 250(2):351-63. DOI: 10.1006/excr.1999.4535. View

Shaban M, Kim S, Idrus R, Khang G . Fibrin and poly(lactic-co-glycolic acid) hybrid scaffold promotes early chondrogenesis of articular chondrocytes: an in vitro study. J Orthop Surg Res. 2008; 3:17. PMC: 2405772. DOI: 10.1186/1749-799X-3-17. View

Zhang L, Zhang X, Li K, Li D, Xiao Y, Fan Y . Icariin promotes extracellular matrix synthesis and gene expression of chondrocytes in vitro. Phytother Res. 2012; 26(9):1385-92. DOI: 10.1002/ptr.3733. View

Croll T, OConnor A, Stevens G, Cooper-White J . Controllable surface modification of poly(lactic-co-glycolic acid) (PLGA) by hydrolysis or aminolysis I: physical, chemical, and theoretical aspects. Biomacromolecules. 2004; 5(2):463-73. DOI: 10.1021/bm0343040. View

Liang W, Lin M, Li X, Li C, Gao B, Gan H . Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line. Int J Mol Med. 2012; 30(4):889-95. DOI: 10.3892/ijmm.2012.1079. View

Lefebvre V, Peeters-Joris C, Vaes G . Production of collagens, collagenase and collagenase inhibitor during the dedifferentiation of articular chondrocytes by serial subcultures. Biochim Biophys Acta. 1990; 1051(3):266-75. DOI: 10.1016/0167-4889(90)90132-w. View

Lee R, Springer M, Shaw R, Ursell P, Blau H . VEGF gene delivery to myocardium: deleterious effects of unregulated expression. Circulation. 2000; 102(8):898-901. DOI: 10.1161/01.cir.102.8.898. View

10.

Wu S, Chang J, Wang C, Wang G, Ho M . Enhancement of chondrogenesis of human adipose derived stem cells in a hyaluronan-enriched microenvironment. Biomaterials. 2009; 31(4):631-40. DOI: 10.1016/j.biomaterials.2009.09.089. View

11.

Mehdikhani-Nahrkhalaji M, Fathi M, Mortazavi V, Mousavi S, Hashemi-Beni B, Razavi S . Novel nanocomposite coating for dental implant applications in vitro and in vivo evaluation. J Mater Sci Mater Med. 2011; 23(2):485-95. DOI: 10.1007/s10856-011-4507-0. View

12.

Duncan R . The dawning era of polymer therapeutics. Nat Rev Drug Discov. 2003; 2(5):347-60. DOI: 10.1038/nrd1088. View

13.

Jockenhoevel S, Zund G, Hoerstrup S, Chalabi K, Sachweh J, Demircan L . Fibrin gel -- advantages of a new scaffold in cardiovascular tissue engineering. Eur J Cardiothorac Surg. 2001; 19(4):424-30. DOI: 10.1016/s1010-7940(01)00624-8. View

14.

Jung Y, Chung Y, Kim S, Tae G, Kim Y, Rhie J . In situ chondrogenic differentiation of human adipose tissue-derived stem cells in a TGF-beta1 loaded fibrin-poly(lactide-caprolactone) nanoparticulate complex. Biomaterials. 2009; 30(27):4657-64. DOI: 10.1016/j.biomaterials.2009.05.034. View

15.

Li D, Yuan T, Xiao Y, Wang R, Fan Y, Zhang X . Icariin: a potential promoting compound for cartilage tissue engineering. Osteoarthritis Cartilage. 2012; 20(12):1647-56. DOI: 10.1016/j.joca.2012.08.009. View

16.

Longobardi L, ORear L, Aakula S, Johnstone B, Shimer K, Chytil A . Effect of IGF-I in the chondrogenesis of bone marrow mesenchymal stem cells in the presence or absence of TGF-beta signaling. J Bone Miner Res. 2006; 21(4):626-36. DOI: 10.1359/jbmr.051213. View

17.

Vedakumari W, Prabu P, Babu S, Sastry T . Fibrin nanoparticles as Possible vehicles for drug delivery. Biochim Biophys Acta. 2013; 1830(8):4244-53. DOI: 10.1016/j.bbagen.2013.04.032. View

18.

van Beuningen H, Glansbeek H, van der Kraan P, van den Berg W . Differential effects of local application of BMP-2 or TGF-beta 1 on both articular cartilage composition and osteophyte formation. Osteoarthritis Cartilage. 1999; 6(5):306-17. DOI: 10.1053/joca.1998.0129. View

19.

Uematsu K, Hattori K, Ishimoto Y, Yamauchi J, Habata T, Takakura Y . Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactic-glycolic acid (PLGA) scaffold. Biomaterials. 2005; 26(20):4273-9. DOI: 10.1016/j.biomaterials.2004.10.037. View

20.

Jiang W, Kim B, Rutka J, Chan W . Advances and challenges of nanotechnology-based drug delivery systems. Expert Opin Drug Deliv. 2007; 4(6):621-33. DOI: 10.1517/17425247.4.6.621. View