Comparison the Effects of Chitosan and Hyaluronic Acid-based Thermally Sensitive Hydrogels Containing Rosuvastatin on Human Osteoblast-like MG-63 Cells

Overview

Journal Res Pharm Sci

Specialty Chemistry

Date 2020 Mar 18

PMID 32180821

Citations 2

Authors

Vajihe Akbari

Mahboubeh Rezazadeh

Zahra Ebrahimi

Affiliations

Soon will be listed here.

Abstract

Background And Purpose: Bone regeneration can be accelerated by localized delivery of statins. Here, we aimed to evaluate the effect of two thermosensitive hydrogels containing rosuvastatin (RSV) on proliferation and differentiation of human osteoblast-like MG-63 cells.

Experimental Approach: Firstly, chitosan (CTS)/glycerophosphate (GP)/gelatin (G) thermosensitive hydrogel was prepared and characterized based on rheological properties, erosion, and release pattern of RSV and then the optimized mixture was loaded with nanoparticles containing RSV(NRSV). Secondly, the effect of NRSV-embedded in CTS/GP/G on cell viability, alkaline phosphate activity, and cell calcification was evaluated using MG-63 cells and compared with RSV-embedded into hyaluronic acid (HA)/Pluronic F127 (PF127) hydrogel.

Findings / Results: CTS/GP mixtures with 1 and 1.5 % gelatin existing in solution with low viscosity at 4 °C were solidified at 32-34 °C while the mixture containing 2% gelatin was jellified at room temperature. The gelation times of CTS/GP/G with 1 and 1.5% gelatin were 72 and 44 s, respectively. The hydrogel containing 3% w/v NRSV was also converted to a semisolid upon increasing the temperature to 33-36 °C. Due to the higher gel strength of CTS/GP/G compared to HA/PF127 hydrogel, the release rate of RSV from the NRSV-embedded CTS/GP/G hydrogel was significantly slower than that of HA/PF127 system. As revealed by alkaline phosphatase and mineralization assays, NRSV-embedded in CTS/GP/G hydrogel had the most promotive effect on differentiation of osteoblasts among other mixtures.

Conclusion And Implication: NRSV-embedded in CTS/GP/G hydrogel could be efficiently used in the future for bone defects such as osteoporosis and bone fractures.

Citing Articles

Fabrication of Rosuvastatin-Incorporated Polycaprolactone -Gelatin Scaffold for Bone Repair: A Preliminary Study.

Gharibshahian M, Alizadeh M, Farahani M, Salehi M Cell J. 2024; 26(1):70-80.

PMID: 38351731 PMC: 10864776. DOI: 10.22074/cellj.2023.2009047.1391.

Hydrogel-based delivery system applied in the local anti-osteoporotic bone defects.

Gong Y, Bu Y, Li Y, Hao D, He B, Kong L Front Bioeng Biotechnol. 2022; 10:1058300.

PMID: 36440439 PMC: 9691673. DOI: 10.3389/fbioe.2022.1058300.

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