Comprehensive Functional Analysis of Ribosomal RNA Methyltransferases

Overview

Journal Front Genet

Date 2020 Mar 17

PMID 32174967

Citations 21

Authors

Philipp Pletnev

Ekaterina Guseva

Anna Zanina

Sergey Evfratov

Margarita Dzama

Vsevolod Treshin

Alexandra Pogorelskaya

Ilya Osterman

Anna Golovina

Maria Rubtsova

Marina Serebryakova

Olga V Pobeguts

Vadim M Govorun

Alexey A Bogdanov

Olga A Dontsova

Petr V Sergiev

Affiliations

Soon will be listed here.

Abstract

Ribosomal RNAs in all organisms are methylated. The functional role of the majority of modified nucleotides is unknown. We systematically questioned the influence of rRNA methylation in on a number of characteristics of bacterial cells with the help of a set of rRNA methyltransferase (MT) gene knockout strains from the Keio collection. Analysis of ribosomal subunits sedimentation profiles of the knockout strains revealed a surprisingly small number of rRNA MT that significantly affected ribosome assembly. Accumulation of the assembly intermediates was observed only for the knockout strain whose growth was retarded most significantly among other rRNA MT knockout strains. Accumulation of the 17S rRNA precursor was observed for () knockout cells as well as for cells devoid of functional and genes. Significant differences were found among the WT and the majority of rRNA MT knockout strains in their ability to sustain exogenous protein overexpression. While the majority of the rRNA MT knockout strains supported suboptimal reporter gene expression, the strain devoid of the gene demonstrated a moderate increase in the yield of ectopic gene expression. Comparative 2D protein gel analysis of rRNA MT knockout strains revealed only minor perturbations of the proteome.

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