Brown Marmorated Stink Bug, Halyomorpha Halys (Stål), Genome: Putative Underpinnings of Polyphagy, Insecticide Resistance Potential and Biology of a Top Worldwide Pest

Background: Halyomorpha halys (Stål), the brown marmorated stink bug, is a highly invasive insect species due in part to its exceptionally high levels of polyphagy. This species is also a nuisance due to overwintering in human-made structures. It has caused significant agricultural losses in recent years along the Atlantic seaboard of North America and in continental Europe. Genomic resources will assist with determining the molecular basis for this species' feeding and habitat traits, defining potential targets for pest management strategies.

Results: Analysis of the 1.15-Gb draft genome assembly has identified a wide variety of genetic elements underpinning the biological characteristics of this formidable pest species, encompassing the roles of sensory functions, digestion, immunity, detoxification and development, all of which likely support H. halys' capacity for invasiveness. Many of the genes identified herein have potential for biomolecular pesticide applications.

Conclusions: Availability of the H. halys genome sequence will be useful for the development of environmentally friendly biomolecular pesticides to be applied in concert with more traditional, synthetic chemical-based controls.

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References

Bansal R, Mian M, Mittapalli O, Michel A . RNA-Seq reveals a xenobiotic stress response in the soybean aphid, Aphis glycines, when fed aphid-resistant soybean. BMC Genomics. 2014; 15:972. PMC: 4289043. DOI: 10.1186/1471-2164-15-972. View

Zhu Y, Yao J, Luttrell R . Identification of Genes Potentially Responsible for extra-Oral Digestion and Overcoming Plant Defense from Salivary Glands of the Tarnished Plant Bug (Hemiptera: Miridae) Using cDNA Sequencing. J Insect Sci. 2016; 16(1). PMC: 4913459. DOI: 10.1093/jisesa/iew041. View

Robinson G, Hackett K, Purcell-Miramontes M, Brown S, Evans J, Goldsmith M . Creating a buzz about insect genomes. Science. 2011; 331(6023):1386. DOI: 10.1126/science.331.6023.1386. View

Wang Y, Wu H, Redei D, Xie Q, Chen Y, Chen P . When did the ancestor of true bugs become stinky? Disentangling the phylogenomics of Hemiptera-Heteroptera. Cladistics. 2021; 35(1):42-66. DOI: 10.1111/cla.12232. View

Briscoe A, Chittka L . The evolution of color vision in insects. Annu Rev Entomol. 2000; 46:471-510. DOI: 10.1146/annurev.ento.46.1.471. View

Nicholson S, Hartson S, Puterka G . Proteomic analysis of secreted saliva from Russian wheat aphid (Diuraphis noxia Kurd.) biotypes that differ in virulence to wheat. J Proteomics. 2012; 75(7):2252-68. DOI: 10.1016/j.jprot.2012.01.031. View

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

Talavera G, Castresana J . Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst Biol. 2007; 56(4):564-77. DOI: 10.1080/10635150701472164. View

Eriksson B, Fredman D, Steiner G, Schmid A . Characterisation and localisation of the opsin protein repertoire in the brain and retinas of a spider and an onychophoran. BMC Evol Biol. 2013; 13:186. PMC: 3851285. DOI: 10.1186/1471-2148-13-186. View

10.

Callaghan A, Guillemaud T, Makate N, Raymond M . Polymorphisms and fluctuations in copy number of amplified esterase genes in Culex pipiens mosquitoes. Insect Mol Biol. 1998; 7(3):295-300. DOI: 10.1111/j.1365-2583.1998.00077.x. View

11.

Field L, Devonshire A . Evidence that the E4 and FE4 esterase genes responsible for insecticide resistance in the aphid Myzus persicae (Sulzer) are part of a gene family. Biochem J. 1998; 330 ( Pt 1):169-73. PMC: 1219123. DOI: 10.1042/bj3300169. View

12.

Oakeshott J, Claudianos C, Russell R, ROBIN G . Carboxyl/cholinesterases: a case study of the evolution of a successful multigene family. Bioessays. 1999; 21(12):1031-42. DOI: 10.1002/(SICI)1521-1878(199912)22:1<1031::AID-BIES7>3.0.CO;2-J. View

13.

Panfilio K, Angelini D . By land, air, and sea: hemipteran diversity through the genomic lens. Curr Opin Insect Sci. 2018; 25:106-115. DOI: 10.1016/j.cois.2017.12.005. View

14.

LARKIN M, Blackshields G, Brown N, Chenna R, McGettigan P, McWilliam H . Clustal W and Clustal X version 2.0. Bioinformatics. 2007; 23(21):2947-8. DOI: 10.1093/bioinformatics/btm404. View

15.

Krumlauf R . Evolution of the vertebrate Hox homeobox genes. Bioessays. 1992; 14(4):245-52. DOI: 10.1002/bies.950140408. View

16.

McKenna D, Scully E, Pauchet Y, Hoover K, Kirsch R, Geib S . Genome of the Asian longhorned beetle (Anoplophora glabripennis), a globally significant invasive species, reveals key functional and evolutionary innovations at the beetle-plant interface. Genome Biol. 2016; 17(1):227. PMC: 5105290. DOI: 10.1186/s13059-016-1088-8. View

17.

Graham L, Davies P . The odorant-binding proteins of Drosophila melanogaster: annotation and characterization of a divergent gene family. Gene. 2002; 292(1-2):43-55. DOI: 10.1016/s0378-1119(02)00672-8. View

18.

Elpidina E, Semashko T, Smirnova Y, Dvoryakova E, Dunaevsky Y, Belozersky M . Direct detection of cysteine peptidases for MALDI-TOF MS analysis using fluorogenic substrates. Anal Biochem. 2018; 567:45-50. DOI: 10.1016/j.ab.2018.12.001. View

19.

Salcedo-Porras N, Guarneri A, Oliveira P, Lowenberger C . Rhodnius prolixus: Identification of missing components of the IMD immune signaling pathway and functional characterization of its role in eliminating bacteria. PLoS One. 2019; 14(4):e0214794. PMC: 6447187. DOI: 10.1371/journal.pone.0214794. View

20.

Turk V, Stoka V, Vasiljeva O, Renko M, Sun T, Turk B . Cysteine cathepsins: from structure, function and regulation to new frontiers. Biochim Biophys Acta. 2011; 1824(1):68-88. PMC: 7105208. DOI: 10.1016/j.bbapap.2011.10.002. View