The Effect of Curcumin and Exercise Rehabilitation on Liver Paraoxonase-1 and NF-kβ Gene Expression in the Rat Induced by Forced Drinking of Ethanol

Overview

Journal Clin Exp Hepatol

Specialty Gastroenterology

Date 2020 Mar 14

PMID 32166124

Citations 1

Authors

Hoseyn Fatolahi

Mohamad Ali Azarbayjani

Maghsoud Peeri

Hasan Matinhomaei

Affiliations

Soon will be listed here.

Abstract

Aim Of The Study: Binge ethanol drinking causes liver damage and decreased paraoxonase-1 (PON-1) gene expression. On the other hand, regular physical activity and curcumin consumption as non-invasive interventions can have liver protective effects through enhancing antioxidant defense, and improving PON-1 and NF-kβ (nuclear factor kappa B) gene expression. The aim of this study was to investigate the interactive effect of exercise rehabilitation and curcumin consumption on hepatocyte damage as well as NF-kβ and PON-1 gene expression in rats.

Material And Methods: Fifty-six male Wistar rats were randomly selected and equally divided into seven groups: dextrose-control (Dext-Con), ethanol-control (Eth-Con), ethanol-saline (Eth-sal), ethanol-DMSO (Eth-DMSO), ethanol-curcumin (Eth-Cur), ethanol-swimming training (Eth-SWT) and ethanol-SWT + curcumin (Eth-SWT + Cur). After four days of the binge drinking protocol followed by six days of quitting, the interventions of SWT and curcumin (50 mg/kg) were employed for 14 days. Afterwards, the rats' liver tissues were collected and sent to the laboratory for biochemical assays.

Results: The interaction of SWT and curcumin caused an increase in PON-1 gene expression ( = 0.02). In addition, curcumin consumption ( = 0.003) and its interaction with SWT ( = 0.004) resulted in a reduction in NF-kβ gene expression. Also, liver tissue damage was observed in the Eth-Con group compared to other groups.

Conclusions: The combination of curcumin and SWT may be used to reduce the side effects of binge ethanol drinking and improve recovery in the quitting period.

Citing Articles

Phytochemicals as Modulators of Paraoxonase-1 in Health and Diseases.

Arab Z, Khayatan D, Razavi S, Zare K, Kheradkhah E, Momtaz S Antioxidants (Basel). 2022; 11(7).

PMID: 35883764 PMC: 9311866. DOI: 10.3390/antiox11071273.

References

Sorenson R, Teiber J, La Du B . The human serum paraoxonase/arylesterase gene (PON1) is one member of a multigene family. Genomics. 1996; 33(3):498-507. DOI: 10.1006/geno.1996.0225. View

Tomas M, Elosua R, Senti M, Molina L, Vila J, Anglada R . Paraoxonase1-192 polymorphism modulates the effects of regular and acute exercise on paraoxonase1 activity. J Lipid Res. 2002; 43(5):713-20. View

Sun Y, Peng M . [Recent advances in curcumin and its derivatives for treatment of liver diseases]. Yao Xue Xue Bao. 2015; 49(11):1483-90. View

Casella-Filho A, Chagas A, Maranhao R, Trombetta I, Cesena F, Silva V . Effect of exercise training on plasma levels and functional properties of high-density lipoprotein cholesterol in the metabolic syndrome. Am J Cardiol. 2011; 107(8):1168-72. DOI: 10.1016/j.amjcard.2010.12.014. View

Samuhasaneeto S, Thong-Ngam D, Kulaputana O, Suyasunanont D, Klaikeaw N . Curcumin decreased oxidative stress, inhibited NF-kappaB activation, and improved liver pathology in ethanol-induced liver injury in rats. J Biomed Biotechnol. 2009; 2009:981963. PMC: 2709731. DOI: 10.1155/2009/981963. View

Ciftci O, Ozdemir I, Tanyildizi S, Yildiz S, Oguzturk H . Antioxidative effects of curcumin, β-myrcene and 1,8-cineole against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in rats liver. Toxicol Ind Health. 2011; 27(5):447-53. DOI: 10.1177/0748233710388452. View

Pfaffl M . A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29(9):e45. PMC: 55695. DOI: 10.1093/nar/29.9.e45. View

Rao M, Marmillot P, Gong M, Palmer D, Seeff L, Strader D . Light, but not heavy alcohol drinking, stimulates paraoxonase by upregulating liver mRNA in rats and humans. Metabolism. 2003; 52(10):1287-94. DOI: 10.1016/s0026-0495(03)00191-4. View

Jafari M, Leaf D, MacRae H, Kasem J, OConner P, Pullinger C . The effects of physical exercise on plasma prebeta-1 high-density lipoprotein. Metabolism. 2003; 52(4):437-42. DOI: 10.1053/meta.2003.50086. View

10.

Otocka-Kmiecik A, Lewandowski M, Szkudlarek U, Nowak D, Orlowska-Majdak M . Aerobic training modulates the effects of exercise-induced oxidative stress on PON1 activity: a preliminary study. ScientificWorldJournal. 2014; 2014:230271. PMC: 4214038. DOI: 10.1155/2014/230271. View

11.

Federico A, Cotticelli G, Festi D, Schiumerini R, Addolorato G, Ferrulli A . The effects of alcohol on gastrointestinal tract, liver and pancreas: evidence-based suggestions for clinical management. Eur Rev Med Pharmacol Sci. 2015; 19(10):1922-40. View

12.

Aicher B, Haser E, Freeman L, Carnie A, Stonik J, Wang X . Diet-induced weight loss in overweight or obese women and changes in high-density lipoprotein levels and function. Obesity (Silver Spring). 2012; 20(10):2057-62. PMC: 3374067. DOI: 10.1038/oby.2012.56. View

13.

Leaf D . The effect of physical exercise on reverse cholesterol transport. Metabolism. 2003; 52(8):950-7. DOI: 10.1016/s0026-0495(03)00147-1. View

14.

Wang M, Chen Y, Chen I, Hsieh S, Chen S, Chiu C . Curcumin protects against thioacetamide-induced hepatic fibrosis by attenuating the inflammatory response and inducing apoptosis of damaged hepatocytes. J Nutr Biochem. 2012; 23(10):1352-66. DOI: 10.1016/j.jnutbio.2011.08.004. View

15.

Schroder H, Marrugat J, Fito M, Weinbrenner T, Covas M . Alcohol consumption is directly associated with circulating oxidized low-density lipoprotein. Free Radic Biol Med. 2006; 40(8):1474-81. DOI: 10.1016/j.freeradbiomed.2005.12.014. View

16.

Nanji A, Jokelainen K, Tipoe G, Rahemtulla A, Thomas P, Dannenberg A . Curcumin prevents alcohol-induced liver disease in rats by inhibiting the expression of NF-kappa B-dependent genes. Am J Physiol Gastrointest Liver Physiol. 2002; 284(2):G321-7. DOI: 10.1152/ajpgi.00230.2002. View

17.

Marsillach J, Ferre N, Vila M, Lligona A, Mackness B, Mackness M . Serum paraoxonase-1 in chronic alcoholics: relationship with liver disease. Clin Biochem. 2007; 40(9-10):645-50. DOI: 10.1016/j.clinbiochem.2007.01.020. View

18.

Van Lenten B, Wagner A, Navab M, Fogelman A . Oxidized phospholipids induce changes in hepatic paraoxonase and ApoJ but not monocyte chemoattractant protein-1 via interleukin-6. J Biol Chem. 2000; 276(3):1923-9. DOI: 10.1074/jbc.M004074200. View

19.

Koncsos P, Seres I, Harangi M, Illyes I, Jozsa L, Gonczi F . Human paraoxonase-1 activity in childhood obesity and its relation to leptin and adiponectin levels. Pediatr Res. 2009; 67(3):309-13. DOI: 10.1203/PDR.0b013e3181c9fb66. View

20.

Gulcubuk A, Altunatmaz K, Sonmez K, Haktanir-Yatkin D, Uzun H, Gurel A . Effects of curcumin on tumour necrosis factor-alpha and interleukin-6 in the late phase of experimental acute pancreatitis. J Vet Med A Physiol Pathol Clin Med. 2006; 53(1):49-54. DOI: 10.1111/j.1439-0442.2006.00786.x. View