Gill Transcriptomes Reveal Expression Changes of Genes Related with Immune and Ion Transport Under Salinity Stress in Silvery Pomfret (Pampus Argenteus)

Overview

Journal Fish Physiol Biochem

Specialty Biochemistry

Date 2020 Mar 13

PMID 32162151

Citations 6

Authors

Juan Li

Liangyi Xue

Mingyue Cao

Yu Zhang

Yajun Wang

Shanliang Xu

Baoxiao Zheng

Zhengjia Lou

Affiliations

Soon will be listed here.

Abstract

Salinity is a major ecological factor in the marine environment, and extremely important for the survival, development, and growth of fish. In this study, gill transcriptomes were examined by high-throughput sequencing at three different salinities (12 ppt as low salinity, 22 ppt as control salinity, and 32 ppt as high salinity) in an importantly economical fish silvery pomfret. A total of 187 genes were differentially expressed, including 111 up-regulated and 76 down-regulated transcripts in low-salinity treatment group and 107 genes differentially expressed, including 74 up-regulated and 33 down-regulated transcripts in high-salinity treatment group compared with the control group, respectively. Some pathways including NOD-like receptor signaling pathway, cytokine-cytokine receptor interaction, Toll-like receptor pathway, cardiac muscle contraction, and vascular smooth muscle contraction were significantly enriched. qPCR analysis further confirmed that mRNA expression levels of immune (HSP90A, IL-1β, TNFα, TLR2, IP-10, MIG, CCL19, and IL-11) and ion transport-related genes (WNK2, NPY2R, CFTR, and SLC4A2) significantly changed under salinity stress. Low salinity stress caused more intensive expression changes of immune-related genes than high salinity. These results imply that salinity stress may affect immune function in addition to regulating osmotic pressure in silvery pomfret.

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References

Abrahamsson C . Neuropeptide Y1- and Y2-receptor-mediated cardiovascular effects in the anesthetized guinea pig, rat, and rabbit. J Cardiovasc Pharmacol. 2000; 36(4):451-8. DOI: 10.1097/00005344-200010000-00006. View

AlMomin S, Kumar V, Al-Amad S, Al-Hussaini M, Dashti T, Al-Enezi K . Draft genome sequence of the silver pomfret fish, Pampus argenteus. Genome. 2015; 59(1):51-8. DOI: 10.1139/gen-2015-0056. View

Alper S . Molecular physiology of SLC4 anion exchangers. Exp Physiol. 2005; 91(1):153-61. DOI: 10.1113/expphysiol.2005.031765. View

Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W . Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17):3389-402. PMC: 146917. DOI: 10.1093/nar/25.17.3389. View

Ashburner M, Ball C, Blake J, Botstein D, Butler H, Cherry J . Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000; 25(1):25-9. PMC: 3037419. DOI: 10.1038/75556. View

Beal A, Martin F, Hale M . Using RNA-seq to determine patterns of sex-bias in gene expression in the brain of the sex-role reversed Gulf Pipefish (Syngnathus scovelli). Mar Genomics. 2017; 37:120-127. DOI: 10.1016/j.margen.2017.09.005. View

Chen T, Wu Y, Chi S . Decreasing salinity of seawater moderates immune response and increases survival rate of giant groupers post betanodavirus infection. Fish Shellfish Immunol. 2016; 57:325-334. DOI: 10.1016/j.fsi.2016.08.050. View

Chen N, Jiang J, Gao X, Li X, Zhang Y, Liu X . Histopathological analysis and the immune related gene expression profiles of mandarin fish (Siniperca chuatsi) infected with Aeromonas hydrophila. Fish Shellfish Immunol. 2018; 83:410-415. DOI: 10.1016/j.fsi.2018.09.023. View

Chen F, Lu X, Nie L, Ning Y, Chen J . Molecular characterization of a CC motif chemokine 19-like gene in ayu (Plecoglossus altivelis) and its role in leukocyte trafficking. Fish Shellfish Immunol. 2017; 72:301-308. DOI: 10.1016/j.fsi.2017.11.012. View

10.

Chen X, Lui E, Ip Y, Lam S . RNA sequencing, de novo assembly and differential analysis of the gill transcriptome of freshwater climbing perch Anabas testudineus after 6 days of seawater exposure. J Fish Biol. 2018; 93(2):215-228. DOI: 10.1111/jfb.13653. View

11.

Cheng Y, Wang R, Xu T . Molecular cloning, characterization and expression analysis of a miiuy croaker (Miichthys miiuy) CXC chemokine gene resembling the CXCL9/CXCL10/CXCL11. Fish Shellfish Immunol. 2011; 31(3):439-45. DOI: 10.1016/j.fsi.2011.06.016. View

12.

Chu W, Zhang F, Song R, Li Y, Wu P, Chen L . Proteomic and microRNA Transcriptome Analysis revealed the microRNA-SmyD1 network regulation in Skeletal Muscle Fibers performance of Chinese perch. Sci Rep. 2017; 7(1):16498. PMC: 5705591. DOI: 10.1038/s41598-017-16718-2. View

13.

Commins S, Borish L, Steinke J . Immunologic messenger molecules: cytokines, interferons, and chemokines. J Allergy Clin Immunol. 2009; 125(2 Suppl 2):S53-72. DOI: 10.1016/j.jaci.2009.07.008. View

14.

Dey R, Ji K, Liu Z, Chen L . A cytokine-cytokine interaction in the assembly of higher-order structure and activation of the interleukine-3:receptor complex. PLoS One. 2009; 4(4):e5188. PMC: 2662821. DOI: 10.1371/journal.pone.0005188. View

15.

Divya P, Gopalakrishnan A, Basheer V, Swaminathan R, Mohitha C, Joy L . Mitochondrial ATPase 6/8 genes to infer the population genetic structure of silver pomfret fish Pampus argenteus along the Indian waters. Mitochondrial DNA. 2014; 26(2):189-94. DOI: 10.3109/19401736.2013.879655. View

16.

Esbaugh A, Grosell M . Esophageal desalination is mediated by Na⁺, H⁺ exchanger-2 in the gulf toadfish (Opsanus beta). Comp Biochem Physiol A Mol Integr Physiol. 2014; 171:57-63. DOI: 10.1016/j.cbpa.2014.02.012. View

17.

Evans D . Teleost fish osmoregulation: what have we learned since August Krogh, Homer Smith, and Ancel Keys. Am J Physiol Regul Integr Comp Physiol. 2008; 295(2):R704-13. DOI: 10.1152/ajpregu.90337.2008. View

18.

Evans D, Piermarini P, Choe K . The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev. 2004; 85(1):97-177. DOI: 10.1152/physrev.00050.2003. View

19.

Finn R, Tate J, Mistry J, Coggill P, Sammut S, Hotz H . The Pfam protein families database. Nucleic Acids Res. 2007; 36(Database issue):D281-8. PMC: 2238907. DOI: 10.1093/nar/gkm960. View

20.

Fox J, Tyler R, Guzzo C, Tuinstra R, Peterson F, Lusso P . Engineering Metamorphic Chemokine Lymphotactin/XCL1 into the GAG-Binding, HIV-Inhibitory Dimer Conformation. ACS Chem Biol. 2015; 10(11):2580-8. PMC: 5028202. DOI: 10.1021/acschembio.5b00542. View