Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity

Overview

Journal Immunity

Publisher Cell Press

Specialty Allergy & Immunology

Date 2020 Mar 12

PMID 32160523

Citations 56

Authors

Thomas M Fenton

Peter B Jorgensen

Kristoffer Niss

Samuel J S Rubin

Urs M Morbe

Lene B Riis

Clement Da Silva

Adam Plumb

Julien Vandamme

Henrik L Jakobsen

Soren Brunak

Aida Habtezion

Ole H Nielsen

Bengt Johansson-Lindbom

William W Agace

Affiliations

Soon will be listed here.

Abstract

The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1 high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.

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