Effect of Chemical Modification in Situ on L-glycerol-3-phosphate Dehydrogenase in Brown Adipose Tissue Mitochondria

Overview

Journal J Bioenerg Biomembr

Publisher Springer

Specialties Biochemistry
Biology
Endocrinology

Date 1988 Oct 1

PMID 3215904

Citations 1

Authors

H Rauchova

Z Beleznai

Z Drahota

Affiliations

Soon will be listed here.

Abstract

Mitochondrial L-glycerol-3-phosphate dehydrogenase (E.C. 1.1.99.5.) was studied by chemical modification in situ with different amino acid side chain specific reagents in mitochondria isolated from hamster brown adipose tissue. The SH-modifying reagents have only slight effect on the enzyme activity. The most effective chemicals were tetranitromethane and diazobenzene sulfonic acid. The enzyme activity can be abolished completely by both of them. In the presence of Ca2+ and/or glycerol-3-phosphate inhibition was greater at the same electrophilic reagent concentration. The effect of Ca2+ and glycerol-3-phosphate is nonadditive on inhibition by these reagents.

Citing Articles

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Oh S, Mai X, Kim J, de Guzman A, Lee J, Park S Exp Mol Med. 2024; 56(5):1066-1079.

PMID: 38689091 PMC: 11148179. DOI: 10.1038/s12276-024-01222-1.

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