Engineering of Glycerol Utilization in Pseudomonas Chlororaphis GP72 for Enhancing Phenazine-1-carboxylic Acid Production

Overview

Journal World J Microbiol Biotechnol

Publisher Springer

Specialties Biotechnology
Microbiology

Date 2020 Mar 12

PMID 32157439

Citations 6

Authors

Chen Song

Sheng-Jie Yue

Wen-Hui Liu

Yi-Fan Zheng

Cheng-Hao Zhang

Tong-Tong Feng

Hong-Bo Hu

Wei Wang

Xue-Hong Zhang

Affiliations

Soon will be listed here.

Abstract

Glycerol is a by-product of biodiesel, and it has a great application prospect to be transformed to synthesize high value-added compounds. Pseudomonas chlororaphis GP72 isolated from the green pepper rhizosphere is a plant growth promoting rhizobacteria that can utilize amount of glycerol to synthesize phenazine-1-carboxylic acid (PCA). PCA has been commercially registered as "Shenqinmycin" in China due to its characteristics of preventing pepper blight and rice sheath blight. The aim of this study was to engineer glycerol utilization pathway in P. chlororaphis GP72. First, the two genes glpF and glpK from the glycerol metabolism pathway were overexpressed in GP72ANO separately. Then, the two genes were co-expressed in GP72ANO, improving PCA production from 729.4 mg/L to 993.4 mg/L at 36 h. Moreover, the shunt pathway was blocked to enhance glycerol utilization, resulting in 1493.3 mg/L PCA production. Additionally, we confirmed the inhibition of glpR on glycerol metabolism pathway in P. chlororaphis GP72. This study provides a good example for improving the utilization of glycerol to synthesize high value-added compounds in Pseudomonas.

Citing Articles

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Li Y, Yue S, Zheng Y, Huang P, Nie Y, Hao X Biology (Basel). 2023; 12(10).

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