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Detection of in Hedgehogs from Central China

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Abstract

is the causative agent of leptospirosis, a zoonotic disease of global importance. To have a better understanding on the host species of , we investigated the prevalence of species in hedgehogs in Central China. Hedgehogs were captured in Hubei Province, China in May and October, 2018. Total DNA was extracted from the kidney tissues of hedgehogs for determining the species by PCR amplification of the , , and genes with genus-specific primers. PCR amplification indicated that the positive rate of hedgehogs to the , , and genes were 19.5% (8/41), 12.2% (5/41), and 9.8% (4/41), respectively. The homology of the partial sequence of , , and genes were 99.0-100% among the strains from hedgehogs. Phylogenetic analysis revealed that species detected in this study clustered together with We detected from hedgehogs in Central China, suggesting hedgehogs are the hosts of

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