Allergen Immunotherapy-Induced Immunoglobulin G4 Reduces Basophil Activation in House Dust Mite-Allergic Asthma Patients

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2020 Mar 11

PMID 32154245

Citations 11

Authors

Mulin Feng

Xiaohui Zeng

Qiujuan Su

Xu Shi

Mo Xian

Rundong Qin

Jing Li

Affiliations

Soon will be listed here.

Abstract

It is unclear if allergen immunotherapy (AIT) can reduce allergy effector cell activation. We evaluated the basophil response during (Der p) subcutaneous immunotherapy (SCIT) and its relationship to allergen-specific immunoglobulin G4 (sIgG4) in allergic rhinitis and/or asthma patients. The study included 55 subjects, of which 35 cases received Der p SCIT and 20 controls received standard medications. Symptom and medication scores (SMSs), sIgG4 levels, specific immunoglobulin E (sIgE) levels, allergen-induced basophil activation tests (BATs) in whole blood, and BAT inhibition assays in serum were determined at weeks 0, 4, 12, 16, 52, and 104 of SCIT. Levels of Der p sIgG4 in SCIT patients significantly increased after 12 weeks of treatment compared to week 0. Serum obtained from SCIT patients significantly inhibited basophil activation after 12 weeks of treatment. Removal of immunoglobulin G4 (IgG4) antibodies at week 104 reduced the ability of serum to block basophil activation. An increase of Der p sIgG4 rather than reduction of Der p sIgE correlated with the reduction of basophil activation during SCIT. The sIgG4 antibodies may compete with sIgE binding to allergens to form an immunoglobulin E (IgE)-allergen complex. SCIT reduced the sensitivity of allergen-triggered basophil activation in Der p allergic rhinitis and/or asthma patients through induction of sIgG4.

Citing Articles

Predicting allergen immunotherapy efficacy based on early maintenance phase response in routine clinical practice.

Qin R, Fu W, Huang R, Xian M, Guo Y, He L World Allergy Organ J. 2024; 17(12):100986.

PMID: 39634515 PMC: 11613417. DOI: 10.1016/j.waojou.2024.100986.

Oral immunotherapy with enteric-coated capsules for allergic rhinitis caused by house dust mites.

Zhang H, Xie W, Zhou W, Chen J, Wang Y, Zhu Y Front Allergy. 2024; 5:1345929.

PMID: 38774588 PMC: 11106396. DOI: 10.3389/falgy.2024.1345929.

Advancements in Allergen Immunotherapy for the Treatment of Atopic Dermatitis.

Guo B, Wu K, Chen C, Lin W, Chang Y, Lin M Int J Mol Sci. 2024; 25(2).

PMID: 38279315 PMC: 10816003. DOI: 10.3390/ijms25021316.

Differences in allergen-specific basophil activation and T cell proliferation in atopic dermatitis patients with comorbid allergic rhinoconjunctivitis treated with a monoclonal anti-IL-4Rα antibody or allergen-specific immunotherapy.

Layritz A, Galicia-Carreon J, Benfadal S, Novak N Immun Inflamm Dis. 2023; 11(4):e808.

PMID: 37102639 PMC: 10091378. DOI: 10.1002/iid3.808.

IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy.

Figo D, Macedo P, Gadermaier G, Remuzgo C, Castro F, Kalil J Int J Mol Sci. 2023; 24(4).

PMID: 36835585 PMC: 9958541. DOI: 10.3390/ijms24044173.

References

Mothes N, Heinzkill M, Drachenberg K, Sperr W, Krauth M, Majlesi Y . Allergen-specific immunotherapy with a monophosphoryl lipid A-adjuvanted vaccine: reduced seasonally boosted immunoglobulin E production and inhibition of basophil histamine release by therapy-induced blocking antibodies. Clin Exp Allergy. 2003; 33(9):1198-208. DOI: 10.1046/j.1365-2222.2003.01699.x. View

Francis J, James L, Paraskevopoulos G, Wong C, Calderon M, Durham S . Grass pollen immunotherapy: IL-10 induction and suppression of late responses precedes IgG4 inhibitory antibody activity. J Allergy Clin Immunol. 2008; 121(5):1120-1125.e2. DOI: 10.1016/j.jaci.2008.01.072. View

Blumberga G, Groes L, Dahl R . SQ-standardized house dust mite immunotherapy as an immunomodulatory treatment in patients with asthma. Allergy. 2010; 66(2):178-85. PMC: 3039748. DOI: 10.1111/j.1398-9995.2010.02451.x. View

Nelson H . Subcutaneous immunotherapy versus sublingual immunotherapy: which is more effective?. J Allergy Clin Immunol Pract. 2014; 2(2):144-9. DOI: 10.1016/j.jaip.2013.11.018. View

Bousquet J, Khaltaev N, Cruz A, Denburg J, Fokkens W, Togias A . Allergic Rhinitis and its Impact on Asthma (ARIA) 2008 update (in collaboration with the World Health Organization, GA(2)LEN and AllerGen). Allergy. 2008; 63 Suppl 86:8-160. DOI: 10.1111/j.1398-9995.2007.01620.x. View

Ebo D, Hagendorens M, Schuerwegh A, Beirens L, Bridts C, De Clerck L . Flow-assisted quantification of in vitro activated basophils in the diagnosis of wasp venom allergy and follow-up of wasp venom immunotherapy. Cytometry B Clin Cytom. 2006; 72(3):196-203. DOI: 10.1002/cyto.b.20142. View

VAN METRE Jr T, Adkinson Jr N, Lichtenstein L, MARDINEY Jr M, Norman Jr P, Rosenberg G . A controlled study of the effectiveness of the Rinkel method of immunotherapy for ragweed pollen hay fever. J Allergy Clin Immunol. 1980; 65(4):288-97. DOI: 10.1016/0091-6749(80)90158-x. View

Devillier P, Fadel R, De Beaumont O . House dust mite sublingual immunotherapy is safe in patients with mild-to-moderate, persistent asthma: a clinical trial. Allergy. 2015; 71(2):249-57. DOI: 10.1111/all.12791. View

Kolbe L, Heusser C, Kolsch E . Isotype-associated recognition of allergen epitopes and its modulation by antigen dose. Immunology. 1995; 84(2):285-9. PMC: 1415099. View

10.

Bodtger U, Ejrnaes A, Hummelshoj L, Jacobi H, Poulsen L, Svenson M . Is immunotherapy-induced birch-pollen-specific IgG4 a marker for decreased allergen-specific sensitivity?. Int Arch Allergy Immunol. 2005; 136(4):340-6. DOI: 10.1159/000084227. View

11.

Durham S, Emminger W, Kapp A, de Monchy J, Rak S, Scadding G . SQ-standardized sublingual grass immunotherapy: confirmation of disease modification 2 years after 3 years of treatment in a randomized trial. J Allergy Clin Immunol. 2012; 129(3):717-725.e5. DOI: 10.1016/j.jaci.2011.12.973. View

12.

Frew A . Allergen immunotherapy. J Allergy Clin Immunol. 2010; 125(2 Suppl 2):S306-13. DOI: 10.1016/j.jaci.2009.10.064. View

13.

Santos A, James L, Bahnson H, Shamji M, Couto-Francisco N, Islam S . IgG4 inhibits peanut-induced basophil and mast cell activation in peanut-tolerant children sensitized to peanut major allergens. J Allergy Clin Immunol. 2015; 135(5):1249-56. PMC: 4418748. DOI: 10.1016/j.jaci.2015.01.012. View

14.

Till S, Francis J, Nouri-Aria K, Durham S . Mechanisms of immunotherapy. J Allergy Clin Immunol. 2004; 113(6):1025-34. DOI: 10.1016/j.jaci.2004.03.024. View

15.

Gonzalez-Munoz M, Villota J, Moneo I . Analysis of basophil activation by flow cytometry in pediatric house dust mite allergy. Pediatr Allergy Immunol. 2008; 19(4):342-7. DOI: 10.1111/j.1399-3038.2007.00656.x. View

16.

Shamji M, Ljorring C, Francis J, Calderon M, Larche M, Kimber I . Functional rather than immunoreactive levels of IgG4 correlate closely with clinical response to grass pollen immunotherapy. Allergy. 2011; 67(2):217-26. DOI: 10.1111/j.1398-9995.2011.02745.x. View

17.

Lai X, Li J, Xiao X, Liu E, Zhang C, Wang H . Specific IgG4 production during house dust mite immunotherapy among age, gender and allergic disease populations. Int Arch Allergy Immunol. 2012; 160(1):37-46. DOI: 10.1159/000339239. View

18.

Michils A, Mairesse M, Ledent C, Gossart B, Baldassarre S, Duchateau J . Modified antigenic reactivity of anti-phospholipase A2 IgG antibodies in patients allergic to bee venom: conversion with immunotherapy and relation to subclass expression. J Allergy Clin Immunol. 1998; 102(1):118-26. DOI: 10.1016/s0091-6749(98)70062-4. View

19.

Arlian L, Rapp C, Fernandez-Caldas E . Allergenicity of Euroglyphus maynei and its cross-reactivity with Dermatophagoides species. J Allergy Clin Immunol. 1993; 91(5):1051-8. DOI: 10.1016/0091-6749(93)90219-6. View

20.

Bidad K, Nawijn M, van Oosterhout A, Van der Heide S, Oude Elberink J . Basophil activation test in the diagnosis and monitoring of mastocytosis patients with wasp venom allergy on immunotherapy. Cytometry B Clin Cytom. 2014; 86(3):183-90. DOI: 10.1002/cyto.b.21148. View